Details
| Original language | English |
|---|---|
| Title of host publication | Acta Horticulturae |
| Publisher | International Society for Horticultural Science |
| Pages | 191-196 |
| Number of pages | 6 |
| ISBN (print) | 9789066054899 |
| Publication status | Published - 31 Jul 2010 |
Publication series
| Name | Acta Horticulturae |
|---|---|
| Volume | 870 |
| ISSN (Print) | 0567-7572 |
Abstract
Rdr1 is a single dominant resistance gene against black spot in roses. For positional cloning of the gene, a BAC library was constructed from a R. multiflora hybrid homozygous for Rdr1. A contig around Rdr1 covering a physical distance of about 200kb was established containing a cluster of nine highly similar resistance gene analogues (RGAs) of the nucleotide binding site (NBS)-leucine rich repeat (LRR) type. To identify the resistance gene among these candidates, expression analyses were performed. We found five of the nine RGAs to be expressed at low, probably constitutive levels in rose leaves, therefore being candidates for Rdr1. In parallel, individual RGAs subcloned into a plant transformation vector were analysed in a heterologous transient expression systems. Eight Rdr 1 homologues were found to be expressed transiently after infiltration into leaves of Nicotiana benthamiana.
Keywords
- Bacterial artificial chromosomes, Gene expression, Resistance gene analogues, Rosa multiflora, TIR-NBS-LRR
ASJC Scopus subject areas
- Agricultural and Biological Sciences(all)
- Horticulture
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Acta Horticulturae. International Society for Horticultural Science, 2010. p. 191-196 (Acta Horticulturae; Vol. 870).
Research output: Chapter in book/report/conference proceeding › Contribution to book/anthology › Research › peer review
}
TY - CHAP
T1 - Cloning and analysis of Rdr1, a black spot resistance gene from roses
AU - Kaufmann, H.
AU - Terefe, D.
AU - Yasmin, A.
AU - Biber, A.
AU - Kuehr, A.
AU - Debener, T.
PY - 2010/7/31
Y1 - 2010/7/31
N2 - Rdr1 is a single dominant resistance gene against black spot in roses. For positional cloning of the gene, a BAC library was constructed from a R. multiflora hybrid homozygous for Rdr1. A contig around Rdr1 covering a physical distance of about 200kb was established containing a cluster of nine highly similar resistance gene analogues (RGAs) of the nucleotide binding site (NBS)-leucine rich repeat (LRR) type. To identify the resistance gene among these candidates, expression analyses were performed. We found five of the nine RGAs to be expressed at low, probably constitutive levels in rose leaves, therefore being candidates for Rdr1. In parallel, individual RGAs subcloned into a plant transformation vector were analysed in a heterologous transient expression systems. Eight Rdr 1 homologues were found to be expressed transiently after infiltration into leaves of Nicotiana benthamiana.
AB - Rdr1 is a single dominant resistance gene against black spot in roses. For positional cloning of the gene, a BAC library was constructed from a R. multiflora hybrid homozygous for Rdr1. A contig around Rdr1 covering a physical distance of about 200kb was established containing a cluster of nine highly similar resistance gene analogues (RGAs) of the nucleotide binding site (NBS)-leucine rich repeat (LRR) type. To identify the resistance gene among these candidates, expression analyses were performed. We found five of the nine RGAs to be expressed at low, probably constitutive levels in rose leaves, therefore being candidates for Rdr1. In parallel, individual RGAs subcloned into a plant transformation vector were analysed in a heterologous transient expression systems. Eight Rdr 1 homologues were found to be expressed transiently after infiltration into leaves of Nicotiana benthamiana.
KW - Bacterial artificial chromosomes
KW - Gene expression
KW - Resistance gene analogues
KW - Rosa multiflora
KW - TIR-NBS-LRR
UR - http://www.scopus.com/inward/record.url?scp=77957127448&partnerID=8YFLogxK
U2 - 10.17660/ActaHortic.2010.870.25
DO - 10.17660/ActaHortic.2010.870.25
M3 - Contribution to book/anthology
AN - SCOPUS:77957127448
SN - 9789066054899
T3 - Acta Horticulturae
SP - 191
EP - 196
BT - Acta Horticulturae
PB - International Society for Horticultural Science
ER -