Details
| Original language | English |
|---|---|
| Pages (from-to) | 4168-4185 |
| Number of pages | 18 |
| Journal | Biotechnology and bioengineering |
| Volume | 118 |
| Issue number | 11 |
| Early online date | 20 Jul 2021 |
| Publication status | Published - 13 Oct 2021 |
Abstract
The field of optogenetics is rapidly growing in relevance and number of developed tools. Among other things, the optogenetic repertoire includes light-responsive ion channels and methods for gene regulation. This review will be confined to the optogenetic control of gene expression in mammalian cells as suitable models for clinical applications. Here optogenetic gene regulation might offer an excellent method for spatially and timely regulated gene and protein expression in cell therapeutic approaches. Well-known systems for gene regulation, such as the LOV-, CRY2/CIB-, PhyB/PIF-systems, as well as other, in mammalian cells not yet fully established systems, will be described. Advantages and disadvantages with regard to clinical applications are outlined in detail. Among the many unanswered questions concerning the application of optogenetics, we discuss items such as the use of exogenous chromophores and their effects on the biology of the cells and methods for a gentle, but effective gene transfection method for optogenetic tools for in vivo applications.
Keywords
- clinical applications, gene and protein regulation, mammalian cells, optogenetics
ASJC Scopus subject areas
- Biochemistry, Genetics and Molecular Biology(all)
- Biotechnology
- Chemical Engineering(all)
- Bioengineering
- Immunology and Microbiology(all)
- Applied Microbiology and Biotechnology
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In: Biotechnology and bioengineering, Vol. 118, No. 11, 13.10.2021, p. 4168-4185.
Research output: Contribution to journal › Review article › Research › peer review
}
TY - JOUR
T1 - Clinical applicability of optogenetic gene regulation
AU - Wichert, Nina
AU - Witt, Martin
AU - Blume, Cornelia
AU - Scheper, Thomas
N1 - Funding Information: This study was funded by the Deutsche Forschungsgemeinschaft (DFG, German Research Foundation) under Germany's Excellence Strategy—EXC 2177/1—Project ID 390895286. Open Access funding enabled and organized by Projekt DEAL.
PY - 2021/10/13
Y1 - 2021/10/13
N2 - The field of optogenetics is rapidly growing in relevance and number of developed tools. Among other things, the optogenetic repertoire includes light-responsive ion channels and methods for gene regulation. This review will be confined to the optogenetic control of gene expression in mammalian cells as suitable models for clinical applications. Here optogenetic gene regulation might offer an excellent method for spatially and timely regulated gene and protein expression in cell therapeutic approaches. Well-known systems for gene regulation, such as the LOV-, CRY2/CIB-, PhyB/PIF-systems, as well as other, in mammalian cells not yet fully established systems, will be described. Advantages and disadvantages with regard to clinical applications are outlined in detail. Among the many unanswered questions concerning the application of optogenetics, we discuss items such as the use of exogenous chromophores and their effects on the biology of the cells and methods for a gentle, but effective gene transfection method for optogenetic tools for in vivo applications.
AB - The field of optogenetics is rapidly growing in relevance and number of developed tools. Among other things, the optogenetic repertoire includes light-responsive ion channels and methods for gene regulation. This review will be confined to the optogenetic control of gene expression in mammalian cells as suitable models for clinical applications. Here optogenetic gene regulation might offer an excellent method for spatially and timely regulated gene and protein expression in cell therapeutic approaches. Well-known systems for gene regulation, such as the LOV-, CRY2/CIB-, PhyB/PIF-systems, as well as other, in mammalian cells not yet fully established systems, will be described. Advantages and disadvantages with regard to clinical applications are outlined in detail. Among the many unanswered questions concerning the application of optogenetics, we discuss items such as the use of exogenous chromophores and their effects on the biology of the cells and methods for a gentle, but effective gene transfection method for optogenetic tools for in vivo applications.
KW - clinical applications
KW - gene and protein regulation
KW - mammalian cells
KW - optogenetics
UR - http://www.scopus.com/inward/record.url?scp=85111534968&partnerID=8YFLogxK
U2 - 10.1002/bit.27895
DO - 10.1002/bit.27895
M3 - Review article
C2 - 34287844
AN - SCOPUS:85111534968
VL - 118
SP - 4168
EP - 4185
JO - Biotechnology and bioengineering
JF - Biotechnology and bioengineering
SN - 0006-3592
IS - 11
ER -