Details
| Original language | English |
|---|---|
| Pages (from-to) | 331-336 |
| Number of pages | 6 |
| Journal | Applied Microbiology and Biotechnology |
| Volume | 62 |
| Issue number | 4 |
| Publication status | Published - 26 Apr 2003 |
Abstract
More than 50 filamentous fungi and yeasts, known for de novo synthesis or biotransformation of mono-, sesqui-, tri-, or tetraterpenes, were screened for their ability to cleave β,β-carotene to flavor compounds. Ten strains discolored a β,β-carotene-containing growth agar, indicating efficient degradation of β,β-carotene. Dihydroactinidiolide was formed as the sole conversion product of β,β-carotene in submerged cultures of Ganoderma applanatum, Hypomyces odoratus, Kuehneromyces mutabilis, and Trametes suaveolens. When mycelium-free culture supernatants from five species were applied for the conversions, nearly complete degradation of β,β-carotene was observed after 12 h. Carotenoid-derived volatile products were detected in the media of Ischnoderma benzoinum, Marasmius scorodonius, and Trametes versicolor. β-Ionone proved to be the main metabolite in each case, whereas β-cyclocitral, dihydroactinidiolide, and 2-hydroxy-2,6,6-trimethylcyclohexanone were formed in minor quantities. Using a photometric bleaching test, the β,β-carotene cleaving enzyme activities of M. scorodonius were partially characterized.
ASJC Scopus subject areas
- Biochemistry, Genetics and Molecular Biology(all)
- Biotechnology
- Immunology and Microbiology(all)
- Applied Microbiology and Biotechnology
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In: Applied Microbiology and Biotechnology, Vol. 62, No. 4, 26.04.2003, p. 331-336.
Research output: Contribution to journal › Article › Research › peer review
}
TY - JOUR
T1 - Cleavage of β,β-carotene to flavor compounds by fungi
AU - Zorn, H.
AU - Langhoff, S.
AU - Scheibner, M.
AU - Berger, R. G.
N1 - Funding information: Support of the work by the Deutsche Forschungsgemeinschaft (ZO 122/1–1) is gratefully acknowledged. All experiments comply with the current laws of the Federal Republic of Germany.
PY - 2003/4/26
Y1 - 2003/4/26
N2 - More than 50 filamentous fungi and yeasts, known for de novo synthesis or biotransformation of mono-, sesqui-, tri-, or tetraterpenes, were screened for their ability to cleave β,β-carotene to flavor compounds. Ten strains discolored a β,β-carotene-containing growth agar, indicating efficient degradation of β,β-carotene. Dihydroactinidiolide was formed as the sole conversion product of β,β-carotene in submerged cultures of Ganoderma applanatum, Hypomyces odoratus, Kuehneromyces mutabilis, and Trametes suaveolens. When mycelium-free culture supernatants from five species were applied for the conversions, nearly complete degradation of β,β-carotene was observed after 12 h. Carotenoid-derived volatile products were detected in the media of Ischnoderma benzoinum, Marasmius scorodonius, and Trametes versicolor. β-Ionone proved to be the main metabolite in each case, whereas β-cyclocitral, dihydroactinidiolide, and 2-hydroxy-2,6,6-trimethylcyclohexanone were formed in minor quantities. Using a photometric bleaching test, the β,β-carotene cleaving enzyme activities of M. scorodonius were partially characterized.
AB - More than 50 filamentous fungi and yeasts, known for de novo synthesis or biotransformation of mono-, sesqui-, tri-, or tetraterpenes, were screened for their ability to cleave β,β-carotene to flavor compounds. Ten strains discolored a β,β-carotene-containing growth agar, indicating efficient degradation of β,β-carotene. Dihydroactinidiolide was formed as the sole conversion product of β,β-carotene in submerged cultures of Ganoderma applanatum, Hypomyces odoratus, Kuehneromyces mutabilis, and Trametes suaveolens. When mycelium-free culture supernatants from five species were applied for the conversions, nearly complete degradation of β,β-carotene was observed after 12 h. Carotenoid-derived volatile products were detected in the media of Ischnoderma benzoinum, Marasmius scorodonius, and Trametes versicolor. β-Ionone proved to be the main metabolite in each case, whereas β-cyclocitral, dihydroactinidiolide, and 2-hydroxy-2,6,6-trimethylcyclohexanone were formed in minor quantities. Using a photometric bleaching test, the β,β-carotene cleaving enzyme activities of M. scorodonius were partially characterized.
UR - http://www.scopus.com/inward/record.url?scp=0042770348&partnerID=8YFLogxK
U2 - 10.1007/s00253-003-1309-4
DO - 10.1007/s00253-003-1309-4
M3 - Article
C2 - 12719936
AN - SCOPUS:0042770348
VL - 62
SP - 331
EP - 336
JO - Applied Microbiology and Biotechnology
JF - Applied Microbiology and Biotechnology
SN - 0175-7598
IS - 4
ER -