Details
| Original language | English |
|---|---|
| Pages (from-to) | 135-144 |
| Number of pages | 10 |
| Journal | Biochemical engineering journal |
| Volume | 22 |
| Issue number | 2 |
| Early online date | 20 Oct 2004 |
| Publication status | Published - Jan 2005 |
Abstract
A two-dimensional (2D) spectrofluorometer is often used to monitor various fermentation processes. The change in fluorescence intensities resulting from various combinations of excitation and emission wavelengths is investigated by using a spectra subtraction technique. But it has a limited capacity to classify the entire fluorescence spectra gathered during fermentations and to extract some useful information from the data. This study shows that the self-organizing map (SOM) is a useful and interpretative method for classification of the entire gamut of fluorescence spectral data and selection of some combinations of excitation and emission wavelengths, which have useful fluorometric information. Some results such as normalized weights and variances indicate that the SOM network is capable of interpreting the fermentation processes of S. cerevisiae and recombinant Escherichia coli monitored by a 2D spectrofluorometer.
Keywords
- 2D spectrofluorometer, Bioprocess monitoring, Fermentation, Self-organizing map, Sensors
ASJC Scopus subject areas
- Biochemistry, Genetics and Molecular Biology(all)
- Biotechnology
- Environmental Science(all)
- Environmental Engineering
- Chemical Engineering(all)
- Bioengineering
- Engineering(all)
- Biomedical Engineering
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In: Biochemical engineering journal, Vol. 22, No. 2, 01.2005, p. 135-144.
Research output: Contribution to journal › Article › Research › peer review
}
TY - JOUR
T1 - Classification of two-dimensional fluorescence spectra using self-organizing maps
AU - Rhee, Jong Il
AU - Lee, Kum Il
AU - Kim, Chun Kwang
AU - Yim, Yong Sik
AU - Chung, Sang Wook
AU - Wei, Jiaqiu
AU - Bellgardt, Karl Heinz
N1 - Funding Information: This work was supported by Grant No. R01-2002-000-00027-0 from the Basic Research Program of the Korea Science & Engineering Foundation (KOSEF).
PY - 2005/1
Y1 - 2005/1
N2 - A two-dimensional (2D) spectrofluorometer is often used to monitor various fermentation processes. The change in fluorescence intensities resulting from various combinations of excitation and emission wavelengths is investigated by using a spectra subtraction technique. But it has a limited capacity to classify the entire fluorescence spectra gathered during fermentations and to extract some useful information from the data. This study shows that the self-organizing map (SOM) is a useful and interpretative method for classification of the entire gamut of fluorescence spectral data and selection of some combinations of excitation and emission wavelengths, which have useful fluorometric information. Some results such as normalized weights and variances indicate that the SOM network is capable of interpreting the fermentation processes of S. cerevisiae and recombinant Escherichia coli monitored by a 2D spectrofluorometer.
AB - A two-dimensional (2D) spectrofluorometer is often used to monitor various fermentation processes. The change in fluorescence intensities resulting from various combinations of excitation and emission wavelengths is investigated by using a spectra subtraction technique. But it has a limited capacity to classify the entire fluorescence spectra gathered during fermentations and to extract some useful information from the data. This study shows that the self-organizing map (SOM) is a useful and interpretative method for classification of the entire gamut of fluorescence spectral data and selection of some combinations of excitation and emission wavelengths, which have useful fluorometric information. Some results such as normalized weights and variances indicate that the SOM network is capable of interpreting the fermentation processes of S. cerevisiae and recombinant Escherichia coli monitored by a 2D spectrofluorometer.
KW - 2D spectrofluorometer
KW - Bioprocess monitoring
KW - Fermentation
KW - Self-organizing map
KW - Sensors
UR - http://www.scopus.com/inward/record.url?scp=11144263731&partnerID=8YFLogxK
U2 - 10.1016/j.bej.2004.09.008
DO - 10.1016/j.bej.2004.09.008
M3 - Article
AN - SCOPUS:11144263731
VL - 22
SP - 135
EP - 144
JO - Biochemical engineering journal
JF - Biochemical engineering journal
SN - 1369-703X
IS - 2
ER -