Details
| Original language | English |
|---|---|
| Article number | 12515 |
| Journal | Scientific reports |
| Volume | 9 |
| Issue number | 1 |
| Early online date | 29 Aug 2019 |
| Publication status | E-pub ahead of print - 29 Aug 2019 |
| Externally published | Yes |
Abstract
Microbial-derived short-chain fatty acids (SCFA) acetate, propionate and butyrate may provide a link between gut microbiota and whole-body insulin sensitivity (IS). In this cross-sectional study (160 participants, 64% male, BMI: 19.2–41.0 kg/m 2, normal or impaired glucose metabolism), associations between SCFA (faecal and fasting circulating) and circulating metabolites, substrate oxidation and IS were investigated. In a subgroup (n = 93), IS was determined using a hyperinsulinemic-euglycemic clamp. Data were analyzed using multiple linear regression analysis adjusted for sex, age and BMI. Fasting circulating acetate, propionate and butyrate concentrations were positively associated with fasting GLP-1 concentrations. Additionally, circulating SCFA were negatively related to whole-body lipolysis (glycerol), triacylglycerols and free fatty acids levels (standardized (std) β adjusted (adj) −0.190, P = 0.023; std β adj −0.202, P = 0.010; std β adj −0.306, P = 0.001, respectively). Circulating acetate and propionate were, respectively, negatively and positively correlated with IS (M-value: std β adj −0.294, P < 0.001; std β adj 0.161, P = 0.033, respectively). We show that circulating rather than faecal SCFA were associated with GLP-1 concentrations, whole-body lipolysis and peripheral IS in humans. Therefore, circulating SCFA are more directly linked to metabolic health, which indicates the need to measure circulating SCFA in human prebiotic/probiotic intervention studies as a biomarker/mediator of effects on host metabolism.
Keywords
- Adult, Aged, Cross-Sectional Studies, Fatty Acids, Volatile/analysis, Feces/chemistry, Female, Gastrointestinal Microbiome, Glucagon-Like Peptide 1/blood, Humans, Insulin Resistance, Insulin/blood, Lipolysis, Male, Middle Aged, Young Adult
ASJC Scopus subject areas
Sustainable Development Goals
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In: Scientific reports, Vol. 9, No. 1, 12515, 29.08.2019.
Research output: Contribution to journal › Article › Research › peer review
}
TY - JOUR
T1 - Circulating but not faecal short-chain fatty acids are related to insulin sensitivity, lipolysis and GLP-1 concentrations in humans
AU - Müller, M.
AU - González Hernández, M.A.
AU - Goossens, G.H.
AU - Reijnders, D.
AU - Holst, J.J.
AU - Jocken, J.W.E.
AU - van Eijk, H.
AU - Canfora, E.E.
AU - Blaak, E.E.
N1 - We would kindly like to thank all study participants, Wendy Sluijsman, Yvonne Essers, Nicole Hoebers, Hasibe Aydeniz and Jos Stegen for their excellent technical support. The studies summarized in this manuscript were fundend by TI Food and Nutrition, a public–private partnership on precompetitive research in food and nutrition research.
PY - 2019/8/29
Y1 - 2019/8/29
N2 - Microbial-derived short-chain fatty acids (SCFA) acetate, propionate and butyrate may provide a link between gut microbiota and whole-body insulin sensitivity (IS). In this cross-sectional study (160 participants, 64% male, BMI: 19.2–41.0 kg/m 2, normal or impaired glucose metabolism), associations between SCFA (faecal and fasting circulating) and circulating metabolites, substrate oxidation and IS were investigated. In a subgroup (n = 93), IS was determined using a hyperinsulinemic-euglycemic clamp. Data were analyzed using multiple linear regression analysis adjusted for sex, age and BMI. Fasting circulating acetate, propionate and butyrate concentrations were positively associated with fasting GLP-1 concentrations. Additionally, circulating SCFA were negatively related to whole-body lipolysis (glycerol), triacylglycerols and free fatty acids levels (standardized (std) β adjusted (adj) −0.190, P = 0.023; std β adj −0.202, P = 0.010; std β adj −0.306, P = 0.001, respectively). Circulating acetate and propionate were, respectively, negatively and positively correlated with IS (M-value: std β adj −0.294, P < 0.001; std β adj 0.161, P = 0.033, respectively). We show that circulating rather than faecal SCFA were associated with GLP-1 concentrations, whole-body lipolysis and peripheral IS in humans. Therefore, circulating SCFA are more directly linked to metabolic health, which indicates the need to measure circulating SCFA in human prebiotic/probiotic intervention studies as a biomarker/mediator of effects on host metabolism.
AB - Microbial-derived short-chain fatty acids (SCFA) acetate, propionate and butyrate may provide a link between gut microbiota and whole-body insulin sensitivity (IS). In this cross-sectional study (160 participants, 64% male, BMI: 19.2–41.0 kg/m 2, normal or impaired glucose metabolism), associations between SCFA (faecal and fasting circulating) and circulating metabolites, substrate oxidation and IS were investigated. In a subgroup (n = 93), IS was determined using a hyperinsulinemic-euglycemic clamp. Data were analyzed using multiple linear regression analysis adjusted for sex, age and BMI. Fasting circulating acetate, propionate and butyrate concentrations were positively associated with fasting GLP-1 concentrations. Additionally, circulating SCFA were negatively related to whole-body lipolysis (glycerol), triacylglycerols and free fatty acids levels (standardized (std) β adjusted (adj) −0.190, P = 0.023; std β adj −0.202, P = 0.010; std β adj −0.306, P = 0.001, respectively). Circulating acetate and propionate were, respectively, negatively and positively correlated with IS (M-value: std β adj −0.294, P < 0.001; std β adj 0.161, P = 0.033, respectively). We show that circulating rather than faecal SCFA were associated with GLP-1 concentrations, whole-body lipolysis and peripheral IS in humans. Therefore, circulating SCFA are more directly linked to metabolic health, which indicates the need to measure circulating SCFA in human prebiotic/probiotic intervention studies as a biomarker/mediator of effects on host metabolism.
KW - Adult
KW - Aged
KW - Cross-Sectional Studies
KW - Fatty Acids, Volatile/analysis
KW - Feces/chemistry
KW - Female
KW - Gastrointestinal Microbiome
KW - Glucagon-Like Peptide 1/blood
KW - Humans
KW - Insulin Resistance
KW - Insulin/blood
KW - Lipolysis
KW - Male
KW - Middle Aged
KW - Young Adult
UR - http://www.scopus.com/inward/record.url?scp=85071622595&partnerID=8YFLogxK
U2 - 10.1038/s41598-019-48775-0
DO - 10.1038/s41598-019-48775-0
M3 - Article
C2 - 31467327
VL - 9
JO - Scientific reports
JF - Scientific reports
SN - 2045-2322
IS - 1
M1 - 12515
ER -