Details
| Original language | English |
|---|---|
| Pages (from-to) | 179-188 |
| Number of pages | 10 |
| Journal | Journal of biotechnology |
| Volume | 105 |
| Issue number | 1-2 |
| Publication status | Published - 5 Sept 2003 |
Abstract
Modern bioprocess control requires fast data acquisition and in-time evaluation of bioprocess variables. On-line fluorescence spectroscopy and the application of chemometric methods accomplish these goals. In order to demonstrate how time-consuming off-line analysis methods can be replaced for bioprocess monitoring, fluorescence measurements were performed during different cultivations of the fungus Claviceps purpurea. To predict process variables like biomass, protein, and alkaloid concentrations, chemometric models were developed on the basis of the acquired fluorescence spectra. The results of these investigations are presented and the applicability of this approach for bioprocess monitoring is discussed.
Keywords
- 2-D fluorescence spectroscopy, Chemometrics, Claviceps purpurea, On-line bioprocess monitoring
ASJC Scopus subject areas
- Biochemistry, Genetics and Molecular Biology(all)
- Biotechnology
- Chemical Engineering(all)
- Bioengineering
- Immunology and Microbiology(all)
- Applied Microbiology and Biotechnology
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In: Journal of biotechnology, Vol. 105, No. 1-2, 05.09.2003, p. 179-188.
Research output: Contribution to journal › Article › Research › peer review
}
TY - JOUR
T1 - Chemometric modelling with two-dimensional fluorescence data for Claviceps purpurea bioprocess characterization
AU - Boehl, Daniela
AU - Solle, Dörte
AU - Hitzmann, Bernd
AU - Scheper, Thomas
PY - 2003/9/5
Y1 - 2003/9/5
N2 - Modern bioprocess control requires fast data acquisition and in-time evaluation of bioprocess variables. On-line fluorescence spectroscopy and the application of chemometric methods accomplish these goals. In order to demonstrate how time-consuming off-line analysis methods can be replaced for bioprocess monitoring, fluorescence measurements were performed during different cultivations of the fungus Claviceps purpurea. To predict process variables like biomass, protein, and alkaloid concentrations, chemometric models were developed on the basis of the acquired fluorescence spectra. The results of these investigations are presented and the applicability of this approach for bioprocess monitoring is discussed.
AB - Modern bioprocess control requires fast data acquisition and in-time evaluation of bioprocess variables. On-line fluorescence spectroscopy and the application of chemometric methods accomplish these goals. In order to demonstrate how time-consuming off-line analysis methods can be replaced for bioprocess monitoring, fluorescence measurements were performed during different cultivations of the fungus Claviceps purpurea. To predict process variables like biomass, protein, and alkaloid concentrations, chemometric models were developed on the basis of the acquired fluorescence spectra. The results of these investigations are presented and the applicability of this approach for bioprocess monitoring is discussed.
KW - 2-D fluorescence spectroscopy
KW - Chemometrics
KW - Claviceps purpurea
KW - On-line bioprocess monitoring
UR - http://www.scopus.com/inward/record.url?scp=0141429032&partnerID=8YFLogxK
U2 - 10.1016/S0168-1656(03)00189-5
DO - 10.1016/S0168-1656(03)00189-5
M3 - Article
C2 - 14511918
AN - SCOPUS:0141429032
VL - 105
SP - 179
EP - 188
JO - Journal of biotechnology
JF - Journal of biotechnology
SN - 0168-1656
IS - 1-2
ER -