Details
| Original language | English |
|---|---|
| Pages (from-to) | 173-177 |
| Number of pages | 5 |
| Journal | BIOINFORMATICS |
| Volume | 19 |
| Issue number | 2 |
| Publication status | Published - 22 Jan 2003 |
Abstract
Motivation: 2D fluorescence spectra provide information from intracellular compounds. Fluorophores like trytophan, tyrosine and phenylalanin as well as NADH and flavins make the corresponding measurement systems very important for bioprocess supervision and control. The evaluation is usually based on chemometric modelling using for their calibration procedure off-line measurements of the desired process variables. Due to the data driven approach lots of off-line measurements are required. Here a methodology is presented, which enables to perform a calibration procedure of chemometric models without any further measurement. Results: The necessary information for the calibration procedure is provided by means of the a priori knowledge about the process, i.e. a mathematical model, whose model parameters are estimated during the calibration procedure, as well as the fact that the substrate should be consumed at the end of the process run. The new methodology for chemometric calibration is applied for a batch cultivation of aerobically grown S. cerevisiae on the glucose Schatzmann medium. As will be presented the chemometric models, which are determined by this method, can be used for prediction during new process runs.
ASJC Scopus subject areas
- Mathematics(all)
- Statistics and Probability
- Biochemistry, Genetics and Molecular Biology(all)
- Biochemistry
- Biochemistry, Genetics and Molecular Biology(all)
- Molecular Biology
- Computer Science(all)
- Computer Science Applications
- Computer Science(all)
- Computational Theory and Mathematics
- Mathematics(all)
- Computational Mathematics
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In: BIOINFORMATICS, Vol. 19, No. 2, 22.01.2003, p. 173-177.
Research output: Contribution to journal › Article › Research › peer review
}
TY - JOUR
T1 - Chemometric Modelling based on 2D-Fluorescence Spectra without a Calibration Measurement
AU - Solle, D.
AU - Geissler, D.
AU - Stärk, E.
AU - Scheper, T.
AU - Hitzmann, B.
PY - 2003/1/22
Y1 - 2003/1/22
N2 - Motivation: 2D fluorescence spectra provide information from intracellular compounds. Fluorophores like trytophan, tyrosine and phenylalanin as well as NADH and flavins make the corresponding measurement systems very important for bioprocess supervision and control. The evaluation is usually based on chemometric modelling using for their calibration procedure off-line measurements of the desired process variables. Due to the data driven approach lots of off-line measurements are required. Here a methodology is presented, which enables to perform a calibration procedure of chemometric models without any further measurement. Results: The necessary information for the calibration procedure is provided by means of the a priori knowledge about the process, i.e. a mathematical model, whose model parameters are estimated during the calibration procedure, as well as the fact that the substrate should be consumed at the end of the process run. The new methodology for chemometric calibration is applied for a batch cultivation of aerobically grown S. cerevisiae on the glucose Schatzmann medium. As will be presented the chemometric models, which are determined by this method, can be used for prediction during new process runs.
AB - Motivation: 2D fluorescence spectra provide information from intracellular compounds. Fluorophores like trytophan, tyrosine and phenylalanin as well as NADH and flavins make the corresponding measurement systems very important for bioprocess supervision and control. The evaluation is usually based on chemometric modelling using for their calibration procedure off-line measurements of the desired process variables. Due to the data driven approach lots of off-line measurements are required. Here a methodology is presented, which enables to perform a calibration procedure of chemometric models without any further measurement. Results: The necessary information for the calibration procedure is provided by means of the a priori knowledge about the process, i.e. a mathematical model, whose model parameters are estimated during the calibration procedure, as well as the fact that the substrate should be consumed at the end of the process run. The new methodology for chemometric calibration is applied for a batch cultivation of aerobically grown S. cerevisiae on the glucose Schatzmann medium. As will be presented the chemometric models, which are determined by this method, can be used for prediction during new process runs.
UR - http://www.scopus.com/inward/record.url?scp=0037316476&partnerID=8YFLogxK
U2 - 10.1093/bioinformatics/19.2.173
DO - 10.1093/bioinformatics/19.2.173
M3 - Article
C2 - 12538236
AN - SCOPUS:0037316476
VL - 19
SP - 173
EP - 177
JO - BIOINFORMATICS
JF - BIOINFORMATICS
SN - 1367-4803
IS - 2
ER -