Details
| Original language | English |
|---|---|
| Number of pages | 12 |
| Journal | Regenerative Biomaterials |
| Volume | 8 |
| Issue number | 5 |
| Early online date | 14 Aug 2021 |
| Publication status | Published - Oct 2021 |
Abstract
Inadequate vascularization leading to insufficient oxygen and nutrient supply in deeper layers of bioartificial tissues remains a limitation in current tissue engineering approaches to which prevascularization offers a promising solution. Hypoxia triggering pre-vascularization by enhanced vascular endothelial growth factor (VEGF) expression can be induced chemically by dimethyloxalylglycine (DMOG). Nanoporous silica nanoparticles (NPSNPs, or mesoporous silica nanoparticles, MSNs) enable sustained delivery of molecules and potentially release DMOG allowing a durable capillarization of a construct. Here we evaluated the effects of soluble DMOG and DMOG-loaded NPSNPs on VEGF secretion of adipose tissue-derived stem cells (ASC) and on tube formation by human umbilical vein endothelial cells (HUVEC)-ASC co-cultures. Repeated doses of 100 mM and 500 mM soluble DMOG on ASC resulted in 3- to 7-fold increased VEGF levels on day 9 (P<0.0001). Same doses of DMOG-NPSNPs enhanced VEGF secretion 7.7-fold (P<0.0001) which could be maintained until day 12 with 500 mM DMOG-NPSNPs. In fibrin-based tube formation assays, 100 mM DMOG-NPSNPs had inhibitory effects whereas 50 mM significantly increased tube length, area and number of junctions transiently for 4 days. Thus, DMOG-NPSNPs supported endothelial tube formation by upregulated VEGF secretion from ASC and thus display a promising tool for prevascularization of tissue-engineered constructs. Further studies will evaluate their effect in hydrogels under perfusion.
Keywords
- Adipose tissue-derived stem cells, Dimethyloxalylglycine, Nanoporous silica nanoparticles, Pre-vascularization, Tissue engineering
ASJC Scopus subject areas
- Materials Science(all)
- Biomaterials
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In: Regenerative Biomaterials, Vol. 8, No. 5, 10.2021.
Research output: Contribution to journal › Article › Research › peer review
}
TY - JOUR
T1 - Chemically induced hypoxia by dimethyloxalylglycine (DMOG)-loaded nanoporous silica nanoparticles supports endothelial tube formation by sustained VEGF release from adipose tissue-derived stem cells
AU - Zippusch, Sarah
AU - Besecke, Karen Fabienne Wilhelmine
AU - Helms, Florian
AU - Klingenberg, Melanie
AU - Lyons, Anne
AU - Behrens, Peter
AU - Haverich, Axel
AU - Wilhelmi, Mathias
AU - Ehlert, Nina
AU - Böer, Ulrike
N1 - Funding Information: This work was supported by the German Society for Implant Research and Development (Funding title “Vascularization of bioartificial implants 2017-2020”) and in part by the Deutsche Forschungsgemeinschaft (DFG, German Research Foundation) under Germany’s Excellence Strategy—EXC 2177/1— Project ID 390895286.
PY - 2021/10
Y1 - 2021/10
N2 - Inadequate vascularization leading to insufficient oxygen and nutrient supply in deeper layers of bioartificial tissues remains a limitation in current tissue engineering approaches to which prevascularization offers a promising solution. Hypoxia triggering pre-vascularization by enhanced vascular endothelial growth factor (VEGF) expression can be induced chemically by dimethyloxalylglycine (DMOG). Nanoporous silica nanoparticles (NPSNPs, or mesoporous silica nanoparticles, MSNs) enable sustained delivery of molecules and potentially release DMOG allowing a durable capillarization of a construct. Here we evaluated the effects of soluble DMOG and DMOG-loaded NPSNPs on VEGF secretion of adipose tissue-derived stem cells (ASC) and on tube formation by human umbilical vein endothelial cells (HUVEC)-ASC co-cultures. Repeated doses of 100 mM and 500 mM soluble DMOG on ASC resulted in 3- to 7-fold increased VEGF levels on day 9 (P<0.0001). Same doses of DMOG-NPSNPs enhanced VEGF secretion 7.7-fold (P<0.0001) which could be maintained until day 12 with 500 mM DMOG-NPSNPs. In fibrin-based tube formation assays, 100 mM DMOG-NPSNPs had inhibitory effects whereas 50 mM significantly increased tube length, area and number of junctions transiently for 4 days. Thus, DMOG-NPSNPs supported endothelial tube formation by upregulated VEGF secretion from ASC and thus display a promising tool for prevascularization of tissue-engineered constructs. Further studies will evaluate their effect in hydrogels under perfusion.
AB - Inadequate vascularization leading to insufficient oxygen and nutrient supply in deeper layers of bioartificial tissues remains a limitation in current tissue engineering approaches to which prevascularization offers a promising solution. Hypoxia triggering pre-vascularization by enhanced vascular endothelial growth factor (VEGF) expression can be induced chemically by dimethyloxalylglycine (DMOG). Nanoporous silica nanoparticles (NPSNPs, or mesoporous silica nanoparticles, MSNs) enable sustained delivery of molecules and potentially release DMOG allowing a durable capillarization of a construct. Here we evaluated the effects of soluble DMOG and DMOG-loaded NPSNPs on VEGF secretion of adipose tissue-derived stem cells (ASC) and on tube formation by human umbilical vein endothelial cells (HUVEC)-ASC co-cultures. Repeated doses of 100 mM and 500 mM soluble DMOG on ASC resulted in 3- to 7-fold increased VEGF levels on day 9 (P<0.0001). Same doses of DMOG-NPSNPs enhanced VEGF secretion 7.7-fold (P<0.0001) which could be maintained until day 12 with 500 mM DMOG-NPSNPs. In fibrin-based tube formation assays, 100 mM DMOG-NPSNPs had inhibitory effects whereas 50 mM significantly increased tube length, area and number of junctions transiently for 4 days. Thus, DMOG-NPSNPs supported endothelial tube formation by upregulated VEGF secretion from ASC and thus display a promising tool for prevascularization of tissue-engineered constructs. Further studies will evaluate their effect in hydrogels under perfusion.
KW - Adipose tissue-derived stem cells
KW - Dimethyloxalylglycine
KW - Nanoporous silica nanoparticles
KW - Pre-vascularization
KW - Tissue engineering
UR - http://www.scopus.com/inward/record.url?scp=85118113195&partnerID=8YFLogxK
U2 - 10.1093/rb/rbab039
DO - 10.1093/rb/rbab039
M3 - Article
AN - SCOPUS:85118113195
VL - 8
JO - Regenerative Biomaterials
JF - Regenerative Biomaterials
SN - 2056-3418
IS - 5
ER -