Details
| Original language | English |
|---|---|
| Pages (from-to) | 1064-1070 |
| Number of pages | 7 |
| Journal | Plant physiology and biochemistry |
| Volume | 49 |
| Issue number | 9 |
| Publication status | Published - 1 Sept 2011 |
Abstract
Sulfurtransferases (Str) comprise a group of enzymes widely distributed in archaea, eubacteria, and eukaryota which catalyze the transfer of a sulfur atom from suitable sulfur donors to nucleophilic sulfur acceptors. Neither the in vivo sulfur donors nor the acceptors of Str could be clearly identified in any of the organisms investigated so far. In Oryza sativa L. 24 Str (OsStr) encoding genes have been identified and subdivided into six groups according to their sequence homology. To half of the Oryza Str a direct homolog and to 40% at least a similar protein in Arabidopsis thaliana (L.) Heynh. could be allocated. Only the group comprising two-domain Str contains more Oryza Str than Arabidopsis Str. According to EST abundance analysis most of the OsStr mRNAs accumulate in several plant tissues. OsStr22, the homolog to the best characterized Str1 from Arabidopsis (AtStr1), shows the highest expression in middle-aged plants whereas AtStr1 shows the highest expression in senescent plants. Heterologously expressed and purified OsStr22 shows very low enzyme activity in comparison to the Arabidopsis and the Brassica napus L. Str. The data obtained so far constitute the basis to analyze differences among the Str family from monocotyledonous and dicotyledonous plants.
Keywords
- 3-Mercaptopyruvate, Oryza, Senescence, Thiosulfate
ASJC Scopus subject areas
- Biochemistry, Genetics and Molecular Biology(all)
- Physiology
- Biochemistry, Genetics and Molecular Biology(all)
- Genetics
- Agricultural and Biological Sciences(all)
- Plant Science
Cite this
- Standard
- Harvard
- Apa
- Vancouver
- BibTeX
- RIS
In: Plant physiology and biochemistry, Vol. 49, No. 9, 01.09.2011, p. 1064-1070.
Research output: Contribution to journal › Article › Research › peer review
}
TY - JOUR
T1 - Characterization of the sulfurtransferase family from Oryza sativa L
AU - Guretzki, Sebastian
AU - Papenbrock, Jutta
N1 - Funding information: We thank Pamela von Trzebiatowski and Julia Volker for their excellent technical assistance and the gardeners for growing the plants. Basic characterization of the rice family was started by Lan Wang in her MSc thesis (2007). The work was financially supported by the Deutsche Forschungsgemeinschaft ( PA 764/1-4 and 5 , PA 764/7-1 , PA 764/11-1 ).
PY - 2011/9/1
Y1 - 2011/9/1
N2 - Sulfurtransferases (Str) comprise a group of enzymes widely distributed in archaea, eubacteria, and eukaryota which catalyze the transfer of a sulfur atom from suitable sulfur donors to nucleophilic sulfur acceptors. Neither the in vivo sulfur donors nor the acceptors of Str could be clearly identified in any of the organisms investigated so far. In Oryza sativa L. 24 Str (OsStr) encoding genes have been identified and subdivided into six groups according to their sequence homology. To half of the Oryza Str a direct homolog and to 40% at least a similar protein in Arabidopsis thaliana (L.) Heynh. could be allocated. Only the group comprising two-domain Str contains more Oryza Str than Arabidopsis Str. According to EST abundance analysis most of the OsStr mRNAs accumulate in several plant tissues. OsStr22, the homolog to the best characterized Str1 from Arabidopsis (AtStr1), shows the highest expression in middle-aged plants whereas AtStr1 shows the highest expression in senescent plants. Heterologously expressed and purified OsStr22 shows very low enzyme activity in comparison to the Arabidopsis and the Brassica napus L. Str. The data obtained so far constitute the basis to analyze differences among the Str family from monocotyledonous and dicotyledonous plants.
AB - Sulfurtransferases (Str) comprise a group of enzymes widely distributed in archaea, eubacteria, and eukaryota which catalyze the transfer of a sulfur atom from suitable sulfur donors to nucleophilic sulfur acceptors. Neither the in vivo sulfur donors nor the acceptors of Str could be clearly identified in any of the organisms investigated so far. In Oryza sativa L. 24 Str (OsStr) encoding genes have been identified and subdivided into six groups according to their sequence homology. To half of the Oryza Str a direct homolog and to 40% at least a similar protein in Arabidopsis thaliana (L.) Heynh. could be allocated. Only the group comprising two-domain Str contains more Oryza Str than Arabidopsis Str. According to EST abundance analysis most of the OsStr mRNAs accumulate in several plant tissues. OsStr22, the homolog to the best characterized Str1 from Arabidopsis (AtStr1), shows the highest expression in middle-aged plants whereas AtStr1 shows the highest expression in senescent plants. Heterologously expressed and purified OsStr22 shows very low enzyme activity in comparison to the Arabidopsis and the Brassica napus L. Str. The data obtained so far constitute the basis to analyze differences among the Str family from monocotyledonous and dicotyledonous plants.
KW - 3-Mercaptopyruvate
KW - Oryza
KW - Senescence
KW - Thiosulfate
UR - http://www.scopus.com/inward/record.url?scp=80052024447&partnerID=8YFLogxK
U2 - 10.1016/j.plaphy.2011.07.010
DO - 10.1016/j.plaphy.2011.07.010
M3 - Article
C2 - 21821426
AN - SCOPUS:80052024447
VL - 49
SP - 1064
EP - 1070
JO - Plant physiology and biochemistry
JF - Plant physiology and biochemistry
SN - 0981-9428
IS - 9
ER -