Details
| Original language | English |
|---|---|
| Pages (from-to) | 2022-2028 |
| Number of pages | 7 |
| Journal | Chemical Engineering and Technology |
| Volume | 34 |
| Issue number | 12 |
| Publication status | Published - 3 Nov 2011 |
Abstract
Compared to conventional protein microarrays, the aptamer microarray is a relatively new and competitive method for analytical applications. The three-dimensional folded aptamers show excellent binding specificity and affinity, and thus, can be used as an alternative to antibodies. Immobilization and binding effects of aptamers under different combinations of surfaces and spacers for aptamer-microarray applications are investigated. In addition, the effects of spacers integrated at the terminal position of aptamers on made in-house and commercially available microarray supports are explored. Microarray surfaces and spacers may affect the binding efficiency and quality between aptamers and their corresponding targets. Various surfaces modified with amino, epoxy, and aldehyde functional groups with short, middle, and long spacers of linear and branched type were investigated. Spacers fused to the terminal end of aptamers were tested and compared with spacers fused on microarray supports.
Keywords
- Aptamer, Immobilization, Microarray
ASJC Scopus subject areas
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In: Chemical Engineering and Technology, Vol. 34, No. 12, 03.11.2011, p. 2022-2028.
Research output: Contribution to journal › Article › Research › peer review
}
TY - JOUR
T1 - Characterization of Optimal AptamerMicroarray Binding Chemistry and Spacer Design
AU - Zhu, G.
AU - Lübbecke, M.
AU - Walter, J. G.
AU - Stahl, F.
AU - Scheper, T.
PY - 2011/11/3
Y1 - 2011/11/3
N2 - Compared to conventional protein microarrays, the aptamer microarray is a relatively new and competitive method for analytical applications. The three-dimensional folded aptamers show excellent binding specificity and affinity, and thus, can be used as an alternative to antibodies. Immobilization and binding effects of aptamers under different combinations of surfaces and spacers for aptamer-microarray applications are investigated. In addition, the effects of spacers integrated at the terminal position of aptamers on made in-house and commercially available microarray supports are explored. Microarray surfaces and spacers may affect the binding efficiency and quality between aptamers and their corresponding targets. Various surfaces modified with amino, epoxy, and aldehyde functional groups with short, middle, and long spacers of linear and branched type were investigated. Spacers fused to the terminal end of aptamers were tested and compared with spacers fused on microarray supports.
AB - Compared to conventional protein microarrays, the aptamer microarray is a relatively new and competitive method for analytical applications. The three-dimensional folded aptamers show excellent binding specificity and affinity, and thus, can be used as an alternative to antibodies. Immobilization and binding effects of aptamers under different combinations of surfaces and spacers for aptamer-microarray applications are investigated. In addition, the effects of spacers integrated at the terminal position of aptamers on made in-house and commercially available microarray supports are explored. Microarray surfaces and spacers may affect the binding efficiency and quality between aptamers and their corresponding targets. Various surfaces modified with amino, epoxy, and aldehyde functional groups with short, middle, and long spacers of linear and branched type were investigated. Spacers fused to the terminal end of aptamers were tested and compared with spacers fused on microarray supports.
KW - Aptamer
KW - Immobilization
KW - Microarray
UR - http://www.scopus.com/inward/record.url?scp=82255181629&partnerID=8YFLogxK
U2 - 10.1002/ceat.201000551
DO - 10.1002/ceat.201000551
M3 - Article
AN - SCOPUS:82255181629
VL - 34
SP - 2022
EP - 2028
JO - Chemical Engineering and Technology
JF - Chemical Engineering and Technology
SN - 0930-7516
IS - 12
ER -