Details
| Original language | English |
|---|---|
| Pages (from-to) | 260-266 |
| Number of pages | 7 |
| Journal | Postharvest biology and technology |
| Volume | 52 |
| Issue number | 3 |
| Early online date | 6 Mar 2009 |
| Publication status | Published - Jun 2009 |
Abstract
Miniature potted roses are sensitive to ethylene and respond to it by bud and leaf abscission, leaf yellowing and/or flower senescence. The goal of this study was to develop a selection strategy for characterization of ethylene sensitivity in rose breeding lines. Two hundred and thirty-three F1 genotypes were obtained from a reciprocal crossing of 'Lavender' and 'Vanilla' cultivars, and evaluated in response to ethylene treatment. Based on ethylene-induced organ abscission, low- and high-sensitive genotypes were selected for physiological and molecular studies compared with the parent plants. After 1 week of ethylene treatment, leaf chroma values and chlorophyll degradation were increased in high-sensitive genotypes, much more than in low-sensitive genotypes. However, after 2 weeks of ethylene treatment, low-sensitive genotypes showed no difference in chroma values, but chlorophyll contents decreased in two out of three genotypes. Expression of ethylene receptor genes (RhETR1/3) and signal transduction genes (RhCTR1/2) was not correlated with ethylene sensitivity of the investigated genotypes. The relative expression of laccase (RhLAC) was evaluated with respect to the relationships of lignin deposition and abscission. Ethylene-induced expression of RhLAC in high-sensitive genotypes increased more strongly than in low-sensitive genotypes. The lowest level of RhLAC transcript was accumulated in pedicels of genotypes that showed the lowest bud abscission. Enhanced expression of the laccase gene by ethylene may be related to the abscission process.
Keywords
- CTR, ETR, F1 hybrid, Laccase, Leaf yellowing, Postharvest quality, RhLAC
ASJC Scopus subject areas
- Agricultural and Biological Sciences(all)
- Food Science
- Agricultural and Biological Sciences(all)
- Agronomy and Crop Science
- Agricultural and Biological Sciences(all)
- Horticulture
Cite this
- Standard
- Harvard
- Apa
- Vancouver
- BibTeX
- RIS
In: Postharvest biology and technology, Vol. 52, No. 3, 06.2009, p. 260-266.
Research output: Contribution to journal › Article › Research › peer review
}
TY - JOUR
T1 - Characterization of ethylene-induced organ abscission in F1 breeding lines of miniature roses (Rosa hybrida L.)
AU - Ahmadi, Noorollah
AU - Mibus, Heiko
AU - Serek, Margrethe
N1 - Funding Information: This research was supported by a grant awarded for PhD study to Noorollah Ahmadi from the Iranian Ministry of Science, Research and Technology. The authors would like to thank Professor Errol Hewett (Massey University, Palmerston North, New Zealand) for linguistic editing of the manuscript.
PY - 2009/6
Y1 - 2009/6
N2 - Miniature potted roses are sensitive to ethylene and respond to it by bud and leaf abscission, leaf yellowing and/or flower senescence. The goal of this study was to develop a selection strategy for characterization of ethylene sensitivity in rose breeding lines. Two hundred and thirty-three F1 genotypes were obtained from a reciprocal crossing of 'Lavender' and 'Vanilla' cultivars, and evaluated in response to ethylene treatment. Based on ethylene-induced organ abscission, low- and high-sensitive genotypes were selected for physiological and molecular studies compared with the parent plants. After 1 week of ethylene treatment, leaf chroma values and chlorophyll degradation were increased in high-sensitive genotypes, much more than in low-sensitive genotypes. However, after 2 weeks of ethylene treatment, low-sensitive genotypes showed no difference in chroma values, but chlorophyll contents decreased in two out of three genotypes. Expression of ethylene receptor genes (RhETR1/3) and signal transduction genes (RhCTR1/2) was not correlated with ethylene sensitivity of the investigated genotypes. The relative expression of laccase (RhLAC) was evaluated with respect to the relationships of lignin deposition and abscission. Ethylene-induced expression of RhLAC in high-sensitive genotypes increased more strongly than in low-sensitive genotypes. The lowest level of RhLAC transcript was accumulated in pedicels of genotypes that showed the lowest bud abscission. Enhanced expression of the laccase gene by ethylene may be related to the abscission process.
AB - Miniature potted roses are sensitive to ethylene and respond to it by bud and leaf abscission, leaf yellowing and/or flower senescence. The goal of this study was to develop a selection strategy for characterization of ethylene sensitivity in rose breeding lines. Two hundred and thirty-three F1 genotypes were obtained from a reciprocal crossing of 'Lavender' and 'Vanilla' cultivars, and evaluated in response to ethylene treatment. Based on ethylene-induced organ abscission, low- and high-sensitive genotypes were selected for physiological and molecular studies compared with the parent plants. After 1 week of ethylene treatment, leaf chroma values and chlorophyll degradation were increased in high-sensitive genotypes, much more than in low-sensitive genotypes. However, after 2 weeks of ethylene treatment, low-sensitive genotypes showed no difference in chroma values, but chlorophyll contents decreased in two out of three genotypes. Expression of ethylene receptor genes (RhETR1/3) and signal transduction genes (RhCTR1/2) was not correlated with ethylene sensitivity of the investigated genotypes. The relative expression of laccase (RhLAC) was evaluated with respect to the relationships of lignin deposition and abscission. Ethylene-induced expression of RhLAC in high-sensitive genotypes increased more strongly than in low-sensitive genotypes. The lowest level of RhLAC transcript was accumulated in pedicels of genotypes that showed the lowest bud abscission. Enhanced expression of the laccase gene by ethylene may be related to the abscission process.
KW - CTR
KW - ETR
KW - F1 hybrid
KW - Laccase
KW - Leaf yellowing
KW - Postharvest quality
KW - RhLAC
UR - http://www.scopus.com/inward/record.url?scp=63249087077&partnerID=8YFLogxK
U2 - 10.1016/j.postharvbio.2008.12.010
DO - 10.1016/j.postharvbio.2008.12.010
M3 - Article
AN - SCOPUS:63249087077
VL - 52
SP - 260
EP - 266
JO - Postharvest biology and technology
JF - Postharvest biology and technology
SN - 0925-5214
IS - 3
ER -