Characterization of Chlamydomonas mutants defective in the H subunit of Mg-chelatase

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  • University of Freiburg
  • Saint Petersburg State University
  • Leibniz Institute of Plant Genetics and Crop Plant Research (IPK)
  • Humboldt-Universität zu Berlin (HU Berlin)
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Details

Original languageEnglish
Pages (from-to)363-373
Number of pages11
JournalMolecular genetics and genomics
Volume266
Issue number3
Publication statusPublished - 14 Nov 2001

Abstract

Two chlorophyll-deficient mutants of Chlamydomonas reinhardtii, chl1 and brs-1, are light sensitive and, when grown heterotrophically in the dark, accumulate protoporphyrin IX and exhibit yellow/orange pigmentation. The lesions in both mutants were mapped to the gene (CHLH) for the plastid-localized H subunit of the heterotrimeric magnesium chelatase that catalyzes the insertion of magnesium into protoporphyrin IX. The genetic defects in the mutants could be assigned to + 1 frameshift mutations in exon 9 (chl1) and exon 10 (brs-1) of the CHLH gene. In both mutants, the H subunit of magnesium chelatase was undetectable, but, as shown for chl1, the steady-state levels of the I and D subunits were unaltered in comparison to wild type. The CHLH gene exhibits marked light inducibility: levels of both the mRNA and the protein product are strongly increased when cultures are shifted from from the dark into the light, suggesting that this protein may play a crucial role in the light regulation of chlorophyll biosynthesis.

Keywords

    Chlamydomonas, CHLH gene, Mg-chelatase, Mutants, Protoporphyrin IX

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Cite this

Characterization of Chlamydomonas mutants defective in the H subunit of Mg-chelatase. / Chekounova, E.; Voronetskaya, V.; Papenbrock, J. et al.
In: Molecular genetics and genomics, Vol. 266, No. 3, 14.11.2001, p. 363-373.

Research output: Contribution to journalArticleResearchpeer review

Chekounova E, Voronetskaya V, Papenbrock J, Grimm B, Beck C. Characterization of Chlamydomonas mutants defective in the H subunit of Mg-chelatase. Molecular genetics and genomics. 2001 Nov 14;266(3):363-373. doi: 10.1007/s004380100574
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title = "Characterization of Chlamydomonas mutants defective in the H subunit of Mg-chelatase",
abstract = "Two chlorophyll-deficient mutants of Chlamydomonas reinhardtii, chl1 and brs-1, are light sensitive and, when grown heterotrophically in the dark, accumulate protoporphyrin IX and exhibit yellow/orange pigmentation. The lesions in both mutants were mapped to the gene (CHLH) for the plastid-localized H subunit of the heterotrimeric magnesium chelatase that catalyzes the insertion of magnesium into protoporphyrin IX. The genetic defects in the mutants could be assigned to + 1 frameshift mutations in exon 9 (chl1) and exon 10 (brs-1) of the CHLH gene. In both mutants, the H subunit of magnesium chelatase was undetectable, but, as shown for chl1, the steady-state levels of the I and D subunits were unaltered in comparison to wild type. The CHLH gene exhibits marked light inducibility: levels of both the mRNA and the protein product are strongly increased when cultures are shifted from from the dark into the light, suggesting that this protein may play a crucial role in the light regulation of chlorophyll biosynthesis.",
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AU - Chekounova, E.

AU - Voronetskaya, V.

AU - Papenbrock, J.

AU - Grimm, B.

AU - Beck, C.

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N2 - Two chlorophyll-deficient mutants of Chlamydomonas reinhardtii, chl1 and brs-1, are light sensitive and, when grown heterotrophically in the dark, accumulate protoporphyrin IX and exhibit yellow/orange pigmentation. The lesions in both mutants were mapped to the gene (CHLH) for the plastid-localized H subunit of the heterotrimeric magnesium chelatase that catalyzes the insertion of magnesium into protoporphyrin IX. The genetic defects in the mutants could be assigned to + 1 frameshift mutations in exon 9 (chl1) and exon 10 (brs-1) of the CHLH gene. In both mutants, the H subunit of magnesium chelatase was undetectable, but, as shown for chl1, the steady-state levels of the I and D subunits were unaltered in comparison to wild type. The CHLH gene exhibits marked light inducibility: levels of both the mRNA and the protein product are strongly increased when cultures are shifted from from the dark into the light, suggesting that this protein may play a crucial role in the light regulation of chlorophyll biosynthesis.

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