Details
| Original language | English |
|---|---|
| Pages (from-to) | 231-245 |
| Number of pages | 15 |
| Journal | Bioengineering |
| Volume | 1 |
| Issue number | 4 |
| Publication status | Published - Dec 2014 |
Abstract
Recruitment of mesenchymal stromal cells (MSC) into the field of tissue engineering is a promising development since these cells can be expanded ex vivo to clinically relevant numbers and, after expansion, retain their ability to differentiate into various cell lineages. Safety requirements and the necessity to obtain high cell numbers without frequent subcultivation of cells raised the question of the possibility of expanding MSC in one-way (single-use) disposable bioreactors. In this study, umbilical cord-derived MSC (UC-MSC) were expanded in a disposable Z®RP 2000 H bioreactor under dynamic conditions. Z®RP was characterized regarding residence time and mixing in order to evaluate the optimal bioreactor settings, enabling optimal mass transfer in the absence of shear stress, allowing an reproducible expansion of MSC, while maintaining their stemness properties. Culture of the UC-MSC in disposable Z®RP 2000 H bioreactor resulted in a reproducible 8-fold increase of cell numbers after 5 days. Cells were shown to maintain specific MSC surface marker expression as well as trilineage differentiation potential and lack stress-induced premature senescence.
Keywords
- Bioreactor, Dynamic culture, MSC expansion, Umbilical cord stem cells
ASJC Scopus subject areas
- Chemical Engineering(all)
- Bioengineering
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In: Bioengineering, Vol. 1, No. 4, 12.2014, p. 231-245.
Research output: Contribution to journal › Article › Research › peer review
}
TY - JOUR
T1 - Characterization and application of a disposable rotating bed bioreactor for mesenchymal stem cell expansion
AU - Neumann, Anne
AU - Lavrentieva, Antonina
AU - Heilkenbrinker, Alexandra
AU - Loenne, Maren
AU - Kasper, Cornelia
N1 - Publisher Copyright: © 2014 by the authors; licensee MDPI, Basel, Switzerland. Copyright: Copyright 2019 Elsevier B.V., All rights reserved.
PY - 2014/12
Y1 - 2014/12
N2 - Recruitment of mesenchymal stromal cells (MSC) into the field of tissue engineering is a promising development since these cells can be expanded ex vivo to clinically relevant numbers and, after expansion, retain their ability to differentiate into various cell lineages. Safety requirements and the necessity to obtain high cell numbers without frequent subcultivation of cells raised the question of the possibility of expanding MSC in one-way (single-use) disposable bioreactors. In this study, umbilical cord-derived MSC (UC-MSC) were expanded in a disposable Z®RP 2000 H bioreactor under dynamic conditions. Z®RP was characterized regarding residence time and mixing in order to evaluate the optimal bioreactor settings, enabling optimal mass transfer in the absence of shear stress, allowing an reproducible expansion of MSC, while maintaining their stemness properties. Culture of the UC-MSC in disposable Z®RP 2000 H bioreactor resulted in a reproducible 8-fold increase of cell numbers after 5 days. Cells were shown to maintain specific MSC surface marker expression as well as trilineage differentiation potential and lack stress-induced premature senescence.
AB - Recruitment of mesenchymal stromal cells (MSC) into the field of tissue engineering is a promising development since these cells can be expanded ex vivo to clinically relevant numbers and, after expansion, retain their ability to differentiate into various cell lineages. Safety requirements and the necessity to obtain high cell numbers without frequent subcultivation of cells raised the question of the possibility of expanding MSC in one-way (single-use) disposable bioreactors. In this study, umbilical cord-derived MSC (UC-MSC) were expanded in a disposable Z®RP 2000 H bioreactor under dynamic conditions. Z®RP was characterized regarding residence time and mixing in order to evaluate the optimal bioreactor settings, enabling optimal mass transfer in the absence of shear stress, allowing an reproducible expansion of MSC, while maintaining their stemness properties. Culture of the UC-MSC in disposable Z®RP 2000 H bioreactor resulted in a reproducible 8-fold increase of cell numbers after 5 days. Cells were shown to maintain specific MSC surface marker expression as well as trilineage differentiation potential and lack stress-induced premature senescence.
KW - Bioreactor
KW - Dynamic culture
KW - MSC expansion
KW - Umbilical cord stem cells
UR - http://www.scopus.com/inward/record.url?scp=85019271125&partnerID=8YFLogxK
U2 - 10.3390/bioengineering1040231
DO - 10.3390/bioengineering1040231
M3 - Article
AN - SCOPUS:85019271125
VL - 1
SP - 231
EP - 245
JO - Bioengineering
JF - Bioengineering
IS - 4
ER -