Details
| Original language | English |
|---|---|
| Title of host publication | Complex Enzymes in Microbial Natural Product Biosynthesis, Part B |
| Subtitle of host publication | Polyketides, Aminocoumarins and Carbohydrates |
| Editors | David Hopwood |
| Pages | 49-78 |
| Number of pages | 30 |
| Edition | B |
| Publication status | Published - 14 Apr 2009 |
| Externally published | Yes |
Publication series
| Name | Methods in Enzymology |
|---|---|
| Number | B |
| Volume | 459 |
| ISSN (Print) | 0076-6879 |
Abstract
Fungi produce a wide variety of biologically active compounds, a large proportion of which are produced by the polyketide biosynthetic pathway. Fungal polyketides comprise a very large and structurally very diverse group, and many display important biological activities, including lovastatin, aflatoxins, and strobilurins. These are produced by very large multifunctional iterative enzymes, the iterative type I polyketide synthases (PKSs) whose closest structural and functional analogues are the mammalian fatty acid synthases. Although fungal polyketides were one of the first classes of secondary metabolites to be subject to extensive biosynthetic studies, they remain the least studied and understood at the enzyme level. This chapter presents an overview of methodologies that have been applied to in vivo and in vitro genetic and biochemical studies on the PKSs responsible for both aromatic and highly reduced polyketide metabolites, and which are providing an improved insight into how these highly complex enzymes function.
ASJC Scopus subject areas
- Biochemistry, Genetics and Molecular Biology(all)
- Biochemistry
- Biochemistry, Genetics and Molecular Biology(all)
- Molecular Biology
Cite this
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Complex Enzymes in Microbial Natural Product Biosynthesis, Part B: Polyketides, Aminocoumarins and Carbohydrates. ed. / David Hopwood. B. ed. 2009. p. 49-78 (Methods in Enzymology; Vol. 459, No. B).
Research output: Chapter in book/report/conference proceeding › Contribution to book/anthology › Research › peer review
}
TY - CHAP
T1 - Chapter 3 Fungal Type I Polyketide Synthases
AU - Cox, Russell J.
AU - Simpson, Thomas J.
PY - 2009/4/14
Y1 - 2009/4/14
N2 - Fungi produce a wide variety of biologically active compounds, a large proportion of which are produced by the polyketide biosynthetic pathway. Fungal polyketides comprise a very large and structurally very diverse group, and many display important biological activities, including lovastatin, aflatoxins, and strobilurins. These are produced by very large multifunctional iterative enzymes, the iterative type I polyketide synthases (PKSs) whose closest structural and functional analogues are the mammalian fatty acid synthases. Although fungal polyketides were one of the first classes of secondary metabolites to be subject to extensive biosynthetic studies, they remain the least studied and understood at the enzyme level. This chapter presents an overview of methodologies that have been applied to in vivo and in vitro genetic and biochemical studies on the PKSs responsible for both aromatic and highly reduced polyketide metabolites, and which are providing an improved insight into how these highly complex enzymes function.
AB - Fungi produce a wide variety of biologically active compounds, a large proportion of which are produced by the polyketide biosynthetic pathway. Fungal polyketides comprise a very large and structurally very diverse group, and many display important biological activities, including lovastatin, aflatoxins, and strobilurins. These are produced by very large multifunctional iterative enzymes, the iterative type I polyketide synthases (PKSs) whose closest structural and functional analogues are the mammalian fatty acid synthases. Although fungal polyketides were one of the first classes of secondary metabolites to be subject to extensive biosynthetic studies, they remain the least studied and understood at the enzyme level. This chapter presents an overview of methodologies that have been applied to in vivo and in vitro genetic and biochemical studies on the PKSs responsible for both aromatic and highly reduced polyketide metabolites, and which are providing an improved insight into how these highly complex enzymes function.
UR - http://www.scopus.com/inward/record.url?scp=64049094079&partnerID=8YFLogxK
U2 - 10.1016/S0076-6879(09)04603-5
DO - 10.1016/S0076-6879(09)04603-5
M3 - Contribution to book/anthology
C2 - 19362635
AN - SCOPUS:64049094079
SN - 9780123745910
T3 - Methods in Enzymology
SP - 49
EP - 78
BT - Complex Enzymes in Microbial Natural Product Biosynthesis, Part B
A2 - Hopwood, David
ER -