Details
| Original language | English |
|---|---|
| Article number | xtac028 |
| Journal | FEMS Microbes |
| Volume | 4 |
| Early online date | 10 Dec 2022 |
| Publication status | Published - 2023 |
Abstract
Determination of the effect of water stress on the surface properties of bacteria is crucial to study bacterial induced soil water repellency. Changes in the environmental conditions may affect several properties of bacteria such as the cell hydrophobicity and morphology. Here, we study the influence of adaptation to hypertonic stress on cell wettability, shape, adhesion, and surface chemical composition of Pseudomonas fluorescens. From this we aim to discover possible relations between the changes in wettability of bacterial films studied by contact angle and single cells studied by atomic and chemical force microscopy (AFM, CFM), which is still lacking. We show that by stress the adhesion forces of the cell surfaces towards hydrophobic functionalized probes increase while they decrease towards hydrophilic functionalized tips. This is consistent with the contact angle results. Further, cell size shrunk and protein content increased upon stress. The results suggest two possible mechanisms: Cell shrinkage is accompanied by the release of outer membrane vesicles by which the protein to lipid ratio increases. The higher protein content increases the rigidity and the number of hydrophobic nano-domains per surface area.
Keywords
- cell surface, chemical force microscopy, hydrophobicity, hypertonic osmotic stress, Pseudomonas fluorescens, X-ray photoelectron spectroscopy
ASJC Scopus subject areas
- Immunology and Microbiology(all)
- Microbiology
- Immunology and Microbiology(all)
- Immunology and Microbiology (miscellaneous)
- Immunology and Microbiology(all)
- Parasitology
- Immunology and Microbiology(all)
- Virology
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In: FEMS Microbes, Vol. 4, xtac028, 2023.
Research output: Contribution to journal › Article › Research › peer review
}
TY - JOUR
T1 - Changes in cell surface properties of Pseudomonas fluorescens by adaptation to NaCl induced hypertonic stress
AU - Abu Quba, Abd Alaziz
AU - Goebel, Marc Oliver
AU - Karagulyan, Mariam
AU - Miltner, Anja
AU - Kästner, Matthias
AU - Bachmann, Jörg
AU - Schaumann, Gabriele E.
AU - Diehl, Doerte
N1 - We are grateful for the funding of the present study by the German Research Foundation (DFG) as part of the project ‘Impact of bacterial biomass on the surface wettability of soil particles under varying moisture conditions’ (GO 2329/2–1/MI 598/4–1/DI 1907/2–1).
PY - 2023
Y1 - 2023
N2 - Determination of the effect of water stress on the surface properties of bacteria is crucial to study bacterial induced soil water repellency. Changes in the environmental conditions may affect several properties of bacteria such as the cell hydrophobicity and morphology. Here, we study the influence of adaptation to hypertonic stress on cell wettability, shape, adhesion, and surface chemical composition of Pseudomonas fluorescens. From this we aim to discover possible relations between the changes in wettability of bacterial films studied by contact angle and single cells studied by atomic and chemical force microscopy (AFM, CFM), which is still lacking. We show that by stress the adhesion forces of the cell surfaces towards hydrophobic functionalized probes increase while they decrease towards hydrophilic functionalized tips. This is consistent with the contact angle results. Further, cell size shrunk and protein content increased upon stress. The results suggest two possible mechanisms: Cell shrinkage is accompanied by the release of outer membrane vesicles by which the protein to lipid ratio increases. The higher protein content increases the rigidity and the number of hydrophobic nano-domains per surface area.
AB - Determination of the effect of water stress on the surface properties of bacteria is crucial to study bacterial induced soil water repellency. Changes in the environmental conditions may affect several properties of bacteria such as the cell hydrophobicity and morphology. Here, we study the influence of adaptation to hypertonic stress on cell wettability, shape, adhesion, and surface chemical composition of Pseudomonas fluorescens. From this we aim to discover possible relations between the changes in wettability of bacterial films studied by contact angle and single cells studied by atomic and chemical force microscopy (AFM, CFM), which is still lacking. We show that by stress the adhesion forces of the cell surfaces towards hydrophobic functionalized probes increase while they decrease towards hydrophilic functionalized tips. This is consistent with the contact angle results. Further, cell size shrunk and protein content increased upon stress. The results suggest two possible mechanisms: Cell shrinkage is accompanied by the release of outer membrane vesicles by which the protein to lipid ratio increases. The higher protein content increases the rigidity and the number of hydrophobic nano-domains per surface area.
KW - cell surface
KW - chemical force microscopy
KW - hydrophobicity
KW - hypertonic osmotic stress
KW - Pseudomonas fluorescens
KW - X-ray photoelectron spectroscopy
UR - http://www.scopus.com/inward/record.url?scp=85173717074&partnerID=8YFLogxK
U2 - 10.1093/femsmc/xtac028
DO - 10.1093/femsmc/xtac028
M3 - Article
AN - SCOPUS:85173717074
VL - 4
JO - FEMS Microbes
JF - FEMS Microbes
M1 - xtac028
ER -