Details
| Original language | English |
|---|---|
| Article number | 8442 |
| Pages (from-to) | 1-19 |
| Number of pages | 19 |
| Journal | International Journal of Molecular Sciences |
| Volume | 21 |
| Issue number | 22 |
| Publication status | Published - 10 Nov 2020 |
Abstract
Cytocompatibility is essential for implant approval. However, initial in vitro screenings mainly include the quantity of adherent immortalized cells and cytotoxicity. Other vital parameters, such as cell migration and an in-depth understanding of the interaction between native tissue cells and implant surfaces, are rarely considered. We investigated different laser-fabricated spike structures using primary and immortalized cell lines of fibroblasts and osteoblasts and included quantification of the cell area, aspect ratio, and focal adhesions. Furthermore, we examined the three-dimensional cell interactions with spike topographies and developed a tailored migration assay for long-term monitoring on opaque materials. While fibroblasts and osteoblasts on small spikes retained their normal morphology, cells on medium and large spikes sank into the structures, affecting the composition of the cytoskeleton and thereby changing cell shape. Up to 14 days, migration appeared stronger on small spikes, probably as a consequence of adequate focal adhesion formation and an intact cytoskeleton, whereas human primary cells revealed differences in comparison to immortalized cell lines. The use of primary cells, analysis of the cell–implant structure interaction as well as cell migration might strengthen the evaluation of cytocompatibility and thereby improve the validity regarding the putative in vivo performance of implant material.
Keywords
- Biomaterials, Cell exclusion assay, Cell proliferation, Cell spreading, Cytocompatibility, Focal adhesion, In vitro screening, Primary vs. immortalized cell lines, Spike structures
ASJC Scopus subject areas
- Chemical Engineering(all)
- Catalysis
- Biochemistry, Genetics and Molecular Biology(all)
- Molecular Biology
- Chemistry(all)
- Spectroscopy
- Computer Science(all)
- Computer Science Applications
- Chemistry(all)
- Physical and Theoretical Chemistry
- Chemistry(all)
- Organic Chemistry
- Chemistry(all)
- Inorganic Chemistry
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In: International Journal of Molecular Sciences, Vol. 21, No. 22, 8442, 10.11.2020, p. 1-19.
Research output: Contribution to journal › Article › Research › peer review
}
TY - JOUR
T1 - Cell Type-Specific Adhesion and Migration on Laser-Structured Opaque Surfaces
AU - Schaeske, Jörn
AU - Fadeeva, Elena
AU - Schlie-Wolter, Sabrina
AU - Deiwick, Andrea
AU - Chichkov, Boris N.
AU - Ingendoh-Tsakmakidis, Alexandra
AU - Stiesch, Meike
AU - Winkel, Andreas
N1 - Funding Information: Acknowledgments: We would like to thank the research association BIOFABRICATION for NIFE, sponsored by the government of Lower Saxony, and the Volkswagenstiftung, for funding this project. We would also like to thank the Research Workshop of the Medical School Hannover for the construction of the frames for the optimized migration assay and Annike Brune for the technical illustration regarding the migration assay in the material and methods section. Funding Information: Niedersächsisches Ministerium für Wissenschaft und Kultur together with Volkswagen Foundation: WVZN2860.
PY - 2020/11/10
Y1 - 2020/11/10
N2 - Cytocompatibility is essential for implant approval. However, initial in vitro screenings mainly include the quantity of adherent immortalized cells and cytotoxicity. Other vital parameters, such as cell migration and an in-depth understanding of the interaction between native tissue cells and implant surfaces, are rarely considered. We investigated different laser-fabricated spike structures using primary and immortalized cell lines of fibroblasts and osteoblasts and included quantification of the cell area, aspect ratio, and focal adhesions. Furthermore, we examined the three-dimensional cell interactions with spike topographies and developed a tailored migration assay for long-term monitoring on opaque materials. While fibroblasts and osteoblasts on small spikes retained their normal morphology, cells on medium and large spikes sank into the structures, affecting the composition of the cytoskeleton and thereby changing cell shape. Up to 14 days, migration appeared stronger on small spikes, probably as a consequence of adequate focal adhesion formation and an intact cytoskeleton, whereas human primary cells revealed differences in comparison to immortalized cell lines. The use of primary cells, analysis of the cell–implant structure interaction as well as cell migration might strengthen the evaluation of cytocompatibility and thereby improve the validity regarding the putative in vivo performance of implant material.
AB - Cytocompatibility is essential for implant approval. However, initial in vitro screenings mainly include the quantity of adherent immortalized cells and cytotoxicity. Other vital parameters, such as cell migration and an in-depth understanding of the interaction between native tissue cells and implant surfaces, are rarely considered. We investigated different laser-fabricated spike structures using primary and immortalized cell lines of fibroblasts and osteoblasts and included quantification of the cell area, aspect ratio, and focal adhesions. Furthermore, we examined the three-dimensional cell interactions with spike topographies and developed a tailored migration assay for long-term monitoring on opaque materials. While fibroblasts and osteoblasts on small spikes retained their normal morphology, cells on medium and large spikes sank into the structures, affecting the composition of the cytoskeleton and thereby changing cell shape. Up to 14 days, migration appeared stronger on small spikes, probably as a consequence of adequate focal adhesion formation and an intact cytoskeleton, whereas human primary cells revealed differences in comparison to immortalized cell lines. The use of primary cells, analysis of the cell–implant structure interaction as well as cell migration might strengthen the evaluation of cytocompatibility and thereby improve the validity regarding the putative in vivo performance of implant material.
KW - Biomaterials
KW - Cell exclusion assay
KW - Cell proliferation
KW - Cell spreading
KW - Cytocompatibility
KW - Focal adhesion
KW - In vitro screening
KW - Primary vs. immortalized cell lines
KW - Spike structures
UR - http://www.scopus.com/inward/record.url?scp=85096023490&partnerID=8YFLogxK
U2 - 10.3390/ijms21228442
DO - 10.3390/ijms21228442
M3 - Article
C2 - 33182746
AN - SCOPUS:85096023490
VL - 21
SP - 1
EP - 19
JO - International Journal of Molecular Sciences
JF - International Journal of Molecular Sciences
SN - 1661-6596
IS - 22
M1 - 8442
ER -