Details
| Original language | English |
|---|---|
| Pages (from-to) | 1191-1199 |
| Journal | Bioprocess and Biosystems Engineering |
| Volume | 38 |
| Issue number | 6 |
| Early online date | 23 Jan 2015 |
| Publication status | Published - Jun 2015 |
Abstract
Four extracellular enzymes, a versatile peroxidase, a manganese peroxidase, a dye-decolorizing peroxidase and a lignin peroxidase were discovered in liquid cultures of the basidiomycete Bjerkandera adusta. All of them cleaved β-carotene effectively. Expression was enhanced in the presence of β-carotene or Coomassie Brilliant Blue and peaked after 7–9 days. The monomeric proteins were purified by ion exchange and size exclusion chromatography and exhibited molecular masses of 41, 43, 51 and 43 kDa, respectively. The coding sequences showed homologies from 61 to 89 % to peroxidases from other basidiomycetes. The novel enzymes retained strong activity even in the absence of hydrogen peroxide and at alkaline pH. De-staining of fabrics using detergent-tolerant enzymes may help to save the most important bio-resources, energy and water, in washing processes and led to green processes in textile cleaning.
Keywords
- Basidiomycota, Carotene degradation, Detergent, Peroxidases
ASJC Scopus subject areas
- Biochemistry, Genetics and Molecular Biology(all)
- Biotechnology
- Chemical Engineering(all)
- Bioengineering
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In: Bioprocess and Biosystems Engineering, Vol. 38, No. 6, 06.2015, p. 1191-1199.
Research output: Contribution to journal › Article › Research › peer review
}
TY - JOUR
T1 - Carotene-degrading activities from Bjerkandera adusta possess an application in detergent industries
AU - Linke, Diana
AU - Leonhardt, Robin
AU - Eisele, Nadine
AU - Petersen, Laura M.
AU - Riemer, Stephanie
AU - Nimtz, Manfred
AU - Berger, Ralf G.
PY - 2015/6
Y1 - 2015/6
N2 - Four extracellular enzymes, a versatile peroxidase, a manganese peroxidase, a dye-decolorizing peroxidase and a lignin peroxidase were discovered in liquid cultures of the basidiomycete Bjerkandera adusta. All of them cleaved β-carotene effectively. Expression was enhanced in the presence of β-carotene or Coomassie Brilliant Blue and peaked after 7–9 days. The monomeric proteins were purified by ion exchange and size exclusion chromatography and exhibited molecular masses of 41, 43, 51 and 43 kDa, respectively. The coding sequences showed homologies from 61 to 89 % to peroxidases from other basidiomycetes. The novel enzymes retained strong activity even in the absence of hydrogen peroxide and at alkaline pH. De-staining of fabrics using detergent-tolerant enzymes may help to save the most important bio-resources, energy and water, in washing processes and led to green processes in textile cleaning.
AB - Four extracellular enzymes, a versatile peroxidase, a manganese peroxidase, a dye-decolorizing peroxidase and a lignin peroxidase were discovered in liquid cultures of the basidiomycete Bjerkandera adusta. All of them cleaved β-carotene effectively. Expression was enhanced in the presence of β-carotene or Coomassie Brilliant Blue and peaked after 7–9 days. The monomeric proteins were purified by ion exchange and size exclusion chromatography and exhibited molecular masses of 41, 43, 51 and 43 kDa, respectively. The coding sequences showed homologies from 61 to 89 % to peroxidases from other basidiomycetes. The novel enzymes retained strong activity even in the absence of hydrogen peroxide and at alkaline pH. De-staining of fabrics using detergent-tolerant enzymes may help to save the most important bio-resources, energy and water, in washing processes and led to green processes in textile cleaning.
KW - Basidiomycota
KW - Carotene degradation
KW - Detergent
KW - Peroxidases
UR - http://www.scopus.com/inward/record.url?scp=85027933972&partnerID=8YFLogxK
U2 - 10.1007/s00449-015-1361-3
DO - 10.1007/s00449-015-1361-3
M3 - Article
C2 - 25614450
AN - SCOPUS:85027933972
VL - 38
SP - 1191
EP - 1199
JO - Bioprocess and Biosystems Engineering
JF - Bioprocess and Biosystems Engineering
SN - 1615-7591
IS - 6
ER -