Details
| Original language | English |
|---|---|
| Article number | 72 |
| Journal | Frontiers in Plant Science |
| Volume | 8 |
| Issue number | FEBRUARY |
| Publication status | Published - 8 Feb 2017 |
Abstract
In eukaryotes the presence of the dimeric phospholipid cardiolipin (CL) is limited to the mitochondrial membranes. It resides predominantly in the inner membrane where it interacts with components of the mitochondrial electron transfer chain. CL deficiency has previously been shown to affect abundances of the plant NADH-dehydrogenase complex and its association with dimeric cyctochrome c reductase. Using an Arabidopsis thaliana knock-out mutant for the final enzyme of CL biosynthesis we here extend current knowledge on the dependence of plant respiration on CL. By correlating respiratory enzyme abundances with enzymatic capacities in mitochondria isolated from wild type, CL deficient and CL complemented heterotrophic cell culture lines a new picture of the participation of CL in plant respiration is emerging. Data indicate a loss of a general reduction of respiratory capacity in CL deficient mitochondria which cannot solely be attributed to decreased abundances or capacities of mitochondrial electron transfer protein complexes and supercomplexes. Instead, it most likely is the result of a loss of the mobile electron carrier cytochrome c. Furthermore, enzymes of the tricarboxylic acid cycle are found to have lower maximum activities in the mutant, including the succinate dehydrogenase complex. Interestingly, abundance of the latter is not altered, indicative of a direct impact of CL deficiency on the enzymatic capacity of this electron transfer chain protein complex.
Keywords
- Cardiolipin, Cytochrome c, Plant mitochondria, Protein complexes, Protein supercomplexes, Respiration
ASJC Scopus subject areas
- Agricultural and Biological Sciences(all)
- Plant Science
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In: Frontiers in Plant Science, Vol. 8, No. FEBRUARY, 72, 08.02.2017.
Research output: Contribution to journal › Article › Research › peer review
}
TY - JOUR
T1 - Cardiolipin Supports Respiratory Enzymes in Plants in Different Ways
AU - Petereit, Jakob
AU - Katayama, Kenta
AU - Lorenz, Christin
AU - Ewert, Linda
AU - Schertl, Peter
AU - Kitsche, Andreas
AU - Wada, Hajime
AU - Frentzen, Margrit
AU - Braun, Hans Peter
AU - Eubel, Holger
N1 - Funding information: We acknowledge financial support by Deutsche Forschungsge-meinschaft (DFG) for MF and HE (EU54/4-1).
PY - 2017/2/8
Y1 - 2017/2/8
N2 - In eukaryotes the presence of the dimeric phospholipid cardiolipin (CL) is limited to the mitochondrial membranes. It resides predominantly in the inner membrane where it interacts with components of the mitochondrial electron transfer chain. CL deficiency has previously been shown to affect abundances of the plant NADH-dehydrogenase complex and its association with dimeric cyctochrome c reductase. Using an Arabidopsis thaliana knock-out mutant for the final enzyme of CL biosynthesis we here extend current knowledge on the dependence of plant respiration on CL. By correlating respiratory enzyme abundances with enzymatic capacities in mitochondria isolated from wild type, CL deficient and CL complemented heterotrophic cell culture lines a new picture of the participation of CL in plant respiration is emerging. Data indicate a loss of a general reduction of respiratory capacity in CL deficient mitochondria which cannot solely be attributed to decreased abundances or capacities of mitochondrial electron transfer protein complexes and supercomplexes. Instead, it most likely is the result of a loss of the mobile electron carrier cytochrome c. Furthermore, enzymes of the tricarboxylic acid cycle are found to have lower maximum activities in the mutant, including the succinate dehydrogenase complex. Interestingly, abundance of the latter is not altered, indicative of a direct impact of CL deficiency on the enzymatic capacity of this electron transfer chain protein complex.
AB - In eukaryotes the presence of the dimeric phospholipid cardiolipin (CL) is limited to the mitochondrial membranes. It resides predominantly in the inner membrane where it interacts with components of the mitochondrial electron transfer chain. CL deficiency has previously been shown to affect abundances of the plant NADH-dehydrogenase complex and its association with dimeric cyctochrome c reductase. Using an Arabidopsis thaliana knock-out mutant for the final enzyme of CL biosynthesis we here extend current knowledge on the dependence of plant respiration on CL. By correlating respiratory enzyme abundances with enzymatic capacities in mitochondria isolated from wild type, CL deficient and CL complemented heterotrophic cell culture lines a new picture of the participation of CL in plant respiration is emerging. Data indicate a loss of a general reduction of respiratory capacity in CL deficient mitochondria which cannot solely be attributed to decreased abundances or capacities of mitochondrial electron transfer protein complexes and supercomplexes. Instead, it most likely is the result of a loss of the mobile electron carrier cytochrome c. Furthermore, enzymes of the tricarboxylic acid cycle are found to have lower maximum activities in the mutant, including the succinate dehydrogenase complex. Interestingly, abundance of the latter is not altered, indicative of a direct impact of CL deficiency on the enzymatic capacity of this electron transfer chain protein complex.
KW - Cardiolipin
KW - Cytochrome c
KW - Plant mitochondria
KW - Protein complexes
KW - Protein supercomplexes
KW - Respiration
UR - http://www.scopus.com/inward/record.url?scp=85011826981&partnerID=8YFLogxK
U2 - 10.3389/fpls.2017.00072
DO - 10.3389/fpls.2017.00072
M3 - Article
AN - SCOPUS:85011826981
VL - 8
JO - Frontiers in Plant Science
JF - Frontiers in Plant Science
SN - 1664-462X
IS - FEBRUARY
M1 - 72
ER -