Details
| Original language | English |
|---|---|
| Pages (from-to) | 205-208 |
| Number of pages | 4 |
| Journal | Biosensors and Bioelectronics |
| Volume | 8 |
| Issue number | 3-4 |
| Publication status | Published - 1993 |
Abstract
A new principle of a calorimetric biosensor for the determination of enantiomeric excesses is described. Two enzymes, immobilized on polymer resins, are used in the system. One hydrolyses only one enantiomer while the other converts both. It is thus possible to determine the concentration of the analyte as well as the ratio of L to D enantiomer. The sensor was tested in aqueous solutions containing up to 50% dimethyl formamide (DMF), and was successfully applied to the analysis of enantiomeric excesses. This result will open novel applications of biosensors for monitoring enzymatic reactions, even when organic solvents are used.
Keywords
- biosensors, calorimetric assay, enantiomeric excess, enzyme engineering, organic phases
ASJC Scopus subject areas
- Biochemistry, Genetics and Molecular Biology(all)
- Biotechnology
- Biochemistry, Genetics and Molecular Biology(all)
- Biophysics
- Engineering(all)
- Biomedical Engineering
- Chemistry(all)
- Electrochemistry
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In: Biosensors and Bioelectronics, Vol. 8, No. 3-4, 1993, p. 205-208.
Research output: Contribution to journal › Article › Research › peer review
}
TY - JOUR
T1 - Calorimetric biosensor for the detection and determination of enantiomeric excesses in aqueous and organic phases
AU - Hundeck, H. G.
AU - Weiß, M.
AU - Scheper, T.
AU - Schubert, F.
PY - 1993
Y1 - 1993
N2 - A new principle of a calorimetric biosensor for the determination of enantiomeric excesses is described. Two enzymes, immobilized on polymer resins, are used in the system. One hydrolyses only one enantiomer while the other converts both. It is thus possible to determine the concentration of the analyte as well as the ratio of L to D enantiomer. The sensor was tested in aqueous solutions containing up to 50% dimethyl formamide (DMF), and was successfully applied to the analysis of enantiomeric excesses. This result will open novel applications of biosensors for monitoring enzymatic reactions, even when organic solvents are used.
AB - A new principle of a calorimetric biosensor for the determination of enantiomeric excesses is described. Two enzymes, immobilized on polymer resins, are used in the system. One hydrolyses only one enantiomer while the other converts both. It is thus possible to determine the concentration of the analyte as well as the ratio of L to D enantiomer. The sensor was tested in aqueous solutions containing up to 50% dimethyl formamide (DMF), and was successfully applied to the analysis of enantiomeric excesses. This result will open novel applications of biosensors for monitoring enzymatic reactions, even when organic solvents are used.
KW - biosensors
KW - calorimetric assay
KW - enantiomeric excess
KW - enzyme engineering
KW - organic phases
UR - http://www.scopus.com/inward/record.url?scp=0027234228&partnerID=8YFLogxK
U2 - 10.1016/0956-5663(93)85034-L
DO - 10.1016/0956-5663(93)85034-L
M3 - Article
AN - SCOPUS:0027234228
VL - 8
SP - 205
EP - 208
JO - Biosensors and Bioelectronics
JF - Biosensors and Bioelectronics
SN - 0956-5663
IS - 3-4
ER -