Details
| Original language | English |
|---|---|
| Pages (from-to) | 2471-2487 |
| Number of pages | 17 |
| Journal | New Phytologist |
| Volume | 233 |
| Issue number | 6 |
| Early online date | 19 Oct 2021 |
| Publication status | Published - 17 Feb 2022 |
Abstract
ALUMINUM-ACTIVATED MALATE TRANSPORTER1 (ALMT1)-mediated malate exudation from roots is critical for aluminium (Al) resistance in Arabidopsis. Its upstream molecular signalling regulation is not yet well understood. The role of CALMODULIN-LIKE24 (CML24) in Al-inhibited root growth and downstream molecular regulation of ALMT1-meditaed Al resistance was investigated. CML24 confers Al resistance demonstrated by an increased root-growth inhibition of the cml24 loss-of-function mutant under Al stress. This occurs mainly through the regulation of the ALMT1-mediated malate exudation from roots. The mutation and overexpression of CML24 leads to an elevated and reduced Al accumulation in the cell wall of roots, respectively. Al stress induced both transcript and protein abundance of CML24 in root tips, especially in the transition zone. CML24 interacts with CALMODULIN BINDING TRANSCRIPTION ACTIVATOR2 (CAMTA2) and promotes its transcriptional activity in the regulation of ALMT1 expression. This results in an enhanced malate exudation from roots and less root-growth inhibition under Al stress. Both CML24 and CAMTA2 interacted with WRKY46 suppressing the transcriptional repression of ALMT1 by WRKY46. The study provides novel insights into understanding of the upstream molecular signalling of the ALMT1-depdendent Al resistance.
Keywords
- ALMT1, Arabidopsis roots, CML24, aluminium, malate exudation
ASJC Scopus subject areas
- Biochemistry, Genetics and Molecular Biology(all)
- Physiology
- Agricultural and Biological Sciences(all)
- Plant Science
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In: New Phytologist, Vol. 233, No. 6, 17.02.2022, p. 2471-2487.
Research output: Contribution to journal › Article › Research › peer review
}
TY - JOUR
T1 - Calmodulin‐like protein CML24 interacts with CAMTA2 and WRKY46 to regulate ALMT1‐dependent Al resistance in Arabidopsis thaliana
AU - Zhu, Xue
AU - Wang, Peng
AU - Bai, Zhimin
AU - Herde, Marco
AU - Ma, Yanqi
AU - Li, Na
AU - Liu, Shuo
AU - Huang, Chao
AU - Cui, Rongxiu
AU - Hongyu, Ma
AU - Zhang, Meng
AU - Wang, Hui
AU - Wei, Tiandi
AU - Quan, Taiyong
AU - Zhang, Wei
AU - Liu, Chunguang
AU - Zhang, Tao
AU - Yang, Zhong Bao
N1 - Funding Information: We thank Prof. Walter J. Horst (Institute of Plant Nutrition, Leibniz University Hannover) for constructive comments on the manuscript, Prof. Shaojian Zheng (Zhejiang University), Prof. Hiroyuki Koyama (Gifu University), Prof. Michael F. Thomashow (Michigan State University), Prof. Kazuko Yamaguchi‐Shinozaki and Satoshi Kidokoro (Tokyo University) for sharing the published materials. This work was financially supported by the National Natural Science Foundation of China (31670259, 31872655, 32070303 and 31400227), Shandong Provincial Key Research and Development Program (2019GSF107005), the Shandong Provincial Natural Science Foundation Project (ZR2014CQ021), and the Fundamental Research Funds of Shandong University (2018JC059). We would like to thank Haiyan Yu, Xiaoming Zhao, Sen Wang and Yuyu Guo from the State Key laboratory of Microbial Technology of Shandong University for assistance with microscopy imaging, and Chengjia Zhang for assistance with Al analysis with GFS‐AAS.
PY - 2022/2/17
Y1 - 2022/2/17
N2 - ALUMINUM-ACTIVATED MALATE TRANSPORTER1 (ALMT1)-mediated malate exudation from roots is critical for aluminium (Al) resistance in Arabidopsis. Its upstream molecular signalling regulation is not yet well understood. The role of CALMODULIN-LIKE24 (CML24) in Al-inhibited root growth and downstream molecular regulation of ALMT1-meditaed Al resistance was investigated. CML24 confers Al resistance demonstrated by an increased root-growth inhibition of the cml24 loss-of-function mutant under Al stress. This occurs mainly through the regulation of the ALMT1-mediated malate exudation from roots. The mutation and overexpression of CML24 leads to an elevated and reduced Al accumulation in the cell wall of roots, respectively. Al stress induced both transcript and protein abundance of CML24 in root tips, especially in the transition zone. CML24 interacts with CALMODULIN BINDING TRANSCRIPTION ACTIVATOR2 (CAMTA2) and promotes its transcriptional activity in the regulation of ALMT1 expression. This results in an enhanced malate exudation from roots and less root-growth inhibition under Al stress. Both CML24 and CAMTA2 interacted with WRKY46 suppressing the transcriptional repression of ALMT1 by WRKY46. The study provides novel insights into understanding of the upstream molecular signalling of the ALMT1-depdendent Al resistance.
AB - ALUMINUM-ACTIVATED MALATE TRANSPORTER1 (ALMT1)-mediated malate exudation from roots is critical for aluminium (Al) resistance in Arabidopsis. Its upstream molecular signalling regulation is not yet well understood. The role of CALMODULIN-LIKE24 (CML24) in Al-inhibited root growth and downstream molecular regulation of ALMT1-meditaed Al resistance was investigated. CML24 confers Al resistance demonstrated by an increased root-growth inhibition of the cml24 loss-of-function mutant under Al stress. This occurs mainly through the regulation of the ALMT1-mediated malate exudation from roots. The mutation and overexpression of CML24 leads to an elevated and reduced Al accumulation in the cell wall of roots, respectively. Al stress induced both transcript and protein abundance of CML24 in root tips, especially in the transition zone. CML24 interacts with CALMODULIN BINDING TRANSCRIPTION ACTIVATOR2 (CAMTA2) and promotes its transcriptional activity in the regulation of ALMT1 expression. This results in an enhanced malate exudation from roots and less root-growth inhibition under Al stress. Both CML24 and CAMTA2 interacted with WRKY46 suppressing the transcriptional repression of ALMT1 by WRKY46. The study provides novel insights into understanding of the upstream molecular signalling of the ALMT1-depdendent Al resistance.
KW - ALMT1
KW - Arabidopsis roots
KW - CML24
KW - aluminium
KW - malate exudation
UR - http://www.scopus.com/inward/record.url?scp=85118488661&partnerID=8YFLogxK
U2 - 10.1111/nph.17812
DO - 10.1111/nph.17812
M3 - Article
VL - 233
SP - 2471
EP - 2487
JO - New Phytologist
JF - New Phytologist
SN - 0028-646X
IS - 6
ER -