Details
| Original language | English |
|---|---|
| Title of host publication | Label-free Biomedical Imaging and Sensing (LBIS) 2023 |
| Editors | Natan T. Shaked, Oliver Hayden |
| Publisher | SPIE |
| ISBN (electronic) | 9781510658875 |
| Publication status | Published - 16 Mar 2023 |
| Event | SPIE Photonics West 2023 - Moscone Center, San Francisco, United States Duration: 28 Jan 2023 → 2 Feb 2023 https://spie.org/conferences-and-exhibitions/photonics-west |
Publication series
| Name | Progress in Biomedical Optics and Imaging - Proceedings of SPIE |
|---|---|
| Volume | 12391 |
| ISSN (Print) | 1605-7422 |
Abstract
Plant cell walls are highly complex structures, which are adaptable to various environmental stresses. While many biochemical pathways have been discovered, cellular stress indicators are widely unknown. Here we demonstrate a label-free optical setup, that can map both mechanical properties and chemical composition in biological samples. We measured changes in mechanical stiffness and chemical response of rose leaf cells due to inoculation of the fungal disease Diplocarpon rosae. With this we are able to image fungal growth in a dynamical and high-resolution manner.
Keywords
- Brillouin scattering, Raman scattering, spectroscopy, plant science, phytophotonics
ASJC Scopus subject areas
- Materials Science(all)
- Electronic, Optical and Magnetic Materials
- Physics and Astronomy(all)
- Atomic and Molecular Physics, and Optics
- Medicine(all)
- Radiology Nuclear Medicine and imaging
- Materials Science(all)
- Biomaterials
Cite this
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Label-free Biomedical Imaging and Sensing (LBIS) 2023. ed. / Natan T. Shaked; Oliver Hayden. SPIE, 2023. 123910K (Progress in Biomedical Optics and Imaging - Proceedings of SPIE; Vol. 12391).
Research output: Chapter in book/report/conference proceeding › Conference contribution › Research › peer review
}
TY - GEN
T1 - Brillouin and Raman imaging for plant cell wall mechanics
AU - Landes, Timm
AU - Weisheit, Inga
AU - Mathew, Alphonse
AU - Alkanat, Eren
AU - Zabic, Miroslav
AU - Debener, Thomas
AU - Heinemann, Dag
N1 - Funding Information: This work is funded by the Deutsche Forschungsgemeinschaft (DFG, German Research Foundation) under Germany’s Excellence Strategy within the Cluster of Excellence PhoenixD (EXC 2122, Project ID 390833453). We also thank Julia Schröter for the support in the biolab.
PY - 2023/3/16
Y1 - 2023/3/16
N2 - Plant cell walls are highly complex structures, which are adaptable to various environmental stresses. While many biochemical pathways have been discovered, cellular stress indicators are widely unknown. Here we demonstrate a label-free optical setup, that can map both mechanical properties and chemical composition in biological samples. We measured changes in mechanical stiffness and chemical response of rose leaf cells due to inoculation of the fungal disease Diplocarpon rosae. With this we are able to image fungal growth in a dynamical and high-resolution manner.
AB - Plant cell walls are highly complex structures, which are adaptable to various environmental stresses. While many biochemical pathways have been discovered, cellular stress indicators are widely unknown. Here we demonstrate a label-free optical setup, that can map both mechanical properties and chemical composition in biological samples. We measured changes in mechanical stiffness and chemical response of rose leaf cells due to inoculation of the fungal disease Diplocarpon rosae. With this we are able to image fungal growth in a dynamical and high-resolution manner.
KW - Brillouin scattering
KW - Raman scattering
KW - spectroscopy
KW - plant science
KW - phytophotonics
UR - http://www.scopus.com/inward/record.url?scp=85159761361&partnerID=8YFLogxK
U2 - 10.1117/12.2649829
DO - 10.1117/12.2649829
M3 - Conference contribution
T3 - Progress in Biomedical Optics and Imaging - Proceedings of SPIE
BT - Label-free Biomedical Imaging and Sensing (LBIS) 2023
A2 - Shaked, Natan T.
A2 - Hayden, Oliver
PB - SPIE
T2 - SPIE Photonics West 2023
Y2 - 28 January 2023 through 2 February 2023
ER -