Details
| Original language | English |
|---|---|
| Pages (from-to) | 395-403 |
| Number of pages | 9 |
| Journal | Sensors and Actuators: B. Chemical |
| Volume | 11 |
| Issue number | 1-3 |
| Publication status | Published - 1 Mar 1993 |
Abstract
The development of a simple but very effective modular fiber-optic fluorimeter is described. Using this instrumentation, different fiber-optic chemo- and biosensors are investigated to demonstrate the application of optical sensors in biotechnology. To obtain information about vital biomass concentrations and the metabolic state of organisms during cultivation, a non-invasive fast-responding on-line sensor based on the combination of turbidometric and spectrofluorometric methods (culture fluorescence monitoring) is presented. In another system, permeabilized cells are confined in front of the fiber tip and changes in the NAD(P)H pool of membrane-bound enzyme complexes inside the cells are monitored during enzymatic reactions with different analytes to study the metabolic activity of the system and to use the system as a biosensor. Another fiber-optic sensor uses the effect of luminescence quenching of a special Ru(II) complex by oxygen to form a sterilizable oxygen optode for measuring dissolved and exhaust-gas oxygen levels. The oxygen optode can also sere as a transducer; various chemo- and biosensors can be constructed by placing a thin layer of a specific catalyst directly onto the oxygen-sensitive membrane. The development of a glucose sensor is used to illustrate this concept.
ASJC Scopus subject areas
- Materials Science(all)
- Electronic, Optical and Magnetic Materials
- Physics and Astronomy(all)
- Instrumentation
- Physics and Astronomy(all)
- Condensed Matter Physics
- Materials Science(all)
- Surfaces, Coatings and Films
- Materials Science(all)
- Metals and Alloys
- Engineering(all)
- Electrical and Electronic Engineering
- Materials Science(all)
- Materials Chemistry
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In: Sensors and Actuators: B. Chemical, Vol. 11, No. 1-3, 01.03.1993, p. 395-403.
Research output: Contribution to journal › Article › Research › peer review
}
TY - JOUR
T1 - Biotechnological applications of fiber-optic sensing: multiple uses of a fiber-optic fluorimeter
AU - Anders, K. D.
AU - Wehnert, G.
AU - Thordsen, O.
AU - Scheper, T.
AU - Rehr, B.
AU - Sahm, H.
PY - 1993/3/1
Y1 - 1993/3/1
N2 - The development of a simple but very effective modular fiber-optic fluorimeter is described. Using this instrumentation, different fiber-optic chemo- and biosensors are investigated to demonstrate the application of optical sensors in biotechnology. To obtain information about vital biomass concentrations and the metabolic state of organisms during cultivation, a non-invasive fast-responding on-line sensor based on the combination of turbidometric and spectrofluorometric methods (culture fluorescence monitoring) is presented. In another system, permeabilized cells are confined in front of the fiber tip and changes in the NAD(P)H pool of membrane-bound enzyme complexes inside the cells are monitored during enzymatic reactions with different analytes to study the metabolic activity of the system and to use the system as a biosensor. Another fiber-optic sensor uses the effect of luminescence quenching of a special Ru(II) complex by oxygen to form a sterilizable oxygen optode for measuring dissolved and exhaust-gas oxygen levels. The oxygen optode can also sere as a transducer; various chemo- and biosensors can be constructed by placing a thin layer of a specific catalyst directly onto the oxygen-sensitive membrane. The development of a glucose sensor is used to illustrate this concept.
AB - The development of a simple but very effective modular fiber-optic fluorimeter is described. Using this instrumentation, different fiber-optic chemo- and biosensors are investigated to demonstrate the application of optical sensors in biotechnology. To obtain information about vital biomass concentrations and the metabolic state of organisms during cultivation, a non-invasive fast-responding on-line sensor based on the combination of turbidometric and spectrofluorometric methods (culture fluorescence monitoring) is presented. In another system, permeabilized cells are confined in front of the fiber tip and changes in the NAD(P)H pool of membrane-bound enzyme complexes inside the cells are monitored during enzymatic reactions with different analytes to study the metabolic activity of the system and to use the system as a biosensor. Another fiber-optic sensor uses the effect of luminescence quenching of a special Ru(II) complex by oxygen to form a sterilizable oxygen optode for measuring dissolved and exhaust-gas oxygen levels. The oxygen optode can also sere as a transducer; various chemo- and biosensors can be constructed by placing a thin layer of a specific catalyst directly onto the oxygen-sensitive membrane. The development of a glucose sensor is used to illustrate this concept.
UR - http://www.scopus.com/inward/record.url?scp=0027554895&partnerID=8YFLogxK
U2 - 10.1016/0925-4005(93)85279-J
DO - 10.1016/0925-4005(93)85279-J
M3 - Article
AN - SCOPUS:0027554895
VL - 11
SP - 395
EP - 403
JO - Sensors and Actuators: B. Chemical
JF - Sensors and Actuators: B. Chemical
SN - 0925-4005
IS - 1-3
ER -