Details
| Original language | English |
|---|---|
| Pages (from-to) | 397-402 |
| Number of pages | 6 |
| Journal | Chemical science |
| Volume | 5 |
| Issue number | 1 |
| Publication status | Published - 1 Jan 2014 |
| Externally published | Yes |
Abstract
The biosynthesis of the mixed PKS-FAS-NRPS hybrid antibiotic thiomarinol A was investigated using feeding studies to both wild type and mutant strains of the marine bacterium Pseudoalteromonas. Particularly interesting features of the pathway include assembly of the 8-hydroxyoctanoic acid side-chain via chain extension of a C4-precursor (4-hydroxybutyrate), and construction of the pyrrothine unit from cysteine via (HolA-D, F-H) prior to intact incorporation into thiomarinol (catalysed by TmlU). A series of thiomarinol-related and other minor metabolites have been isolated from wild-type and mutant strains. The results of these investigations are rationalised in terms of the overall biosynthetic pathway.
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In: Chemical science, Vol. 5, No. 1, 01.01.2014, p. 397-402.
Research output: Contribution to journal › Article › Research › peer review
}
TY - JOUR
T1 - Biosynthesis of thiomarinol A and related metabolites of Pseudoalteromonas sp. SANK 73390
AU - Murphy, Annabel C.
AU - Gao, Shu Shan
AU - Han, Li Chen
AU - Carobene, Simon
AU - Fukuda, Daisuke
AU - Song, Zhongshu
AU - Hothersall, Joanne
AU - Cox, Russell J.
AU - Crosby, John
AU - Crump, Matthew P.
AU - Thomas, Christopher M.
AU - Willis, Christine L.
AU - Simpson, Thomas J.
PY - 2014/1/1
Y1 - 2014/1/1
N2 - The biosynthesis of the mixed PKS-FAS-NRPS hybrid antibiotic thiomarinol A was investigated using feeding studies to both wild type and mutant strains of the marine bacterium Pseudoalteromonas. Particularly interesting features of the pathway include assembly of the 8-hydroxyoctanoic acid side-chain via chain extension of a C4-precursor (4-hydroxybutyrate), and construction of the pyrrothine unit from cysteine via (HolA-D, F-H) prior to intact incorporation into thiomarinol (catalysed by TmlU). A series of thiomarinol-related and other minor metabolites have been isolated from wild-type and mutant strains. The results of these investigations are rationalised in terms of the overall biosynthetic pathway.
AB - The biosynthesis of the mixed PKS-FAS-NRPS hybrid antibiotic thiomarinol A was investigated using feeding studies to both wild type and mutant strains of the marine bacterium Pseudoalteromonas. Particularly interesting features of the pathway include assembly of the 8-hydroxyoctanoic acid side-chain via chain extension of a C4-precursor (4-hydroxybutyrate), and construction of the pyrrothine unit from cysteine via (HolA-D, F-H) prior to intact incorporation into thiomarinol (catalysed by TmlU). A series of thiomarinol-related and other minor metabolites have been isolated from wild-type and mutant strains. The results of these investigations are rationalised in terms of the overall biosynthetic pathway.
UR - http://www.scopus.com/inward/record.url?scp=84888634135&partnerID=8YFLogxK
U2 - 10.1039/c3sc52281d
DO - 10.1039/c3sc52281d
M3 - Article
AN - SCOPUS:84888634135
VL - 5
SP - 397
EP - 402
JO - Chemical science
JF - Chemical science
SN - 2041-6520
IS - 1
ER -