Details
| Original language | English |
|---|---|
| Article number | 777474 |
| Journal | Frontiers in Fungal Biology |
| Volume | 2 |
| Publication status | Published - 3 Jan 2022 |
| Externally published | Yes |
Abstract
The soil microbiome comprises numerous filamentous fungi and bacteria that mutually react and challenge each other by the production of bioactive secondary metabolites. Herein, we show in liquid co-cultures that the presence of filamentous Streptomycetes producing antifungal glycopeptide antibiotics induces the production of the antibacterial and iron-chelating tropolones anhydrosepedonin (1) and antibiotic C (2) in the mold Aspergillus nidulans. Additionally, the biosynthesis of the related polyketide tripyrnidone (5) was induced, whose novel tricyclic scaffold we elucidated by NMR and HRESIMS data. The corresponding biosynthetic polyketide synthase-encoding gene cluster responsible for the production of these compounds was identified. The tropolones as well as tripyrnidone (5) are produced by genes that belong to the broad reservoir of the fungal genome for the synthesis of different secondary metabolites, which are usually silenced under standard laboratory conditions. These molecules might be part of the bacterium-fungus competition in the complex soil environment, with the bacterial glycopeptide antibiotic as specific environmental trigger for fungal induction of this cluster.
Keywords
- Aspergillus nidulans, fungal-bacterial co-cultivation, glycopeptide antibiotics, Streptomyces, structure elucidation, tropolones
ASJC Scopus subject areas
- Environmental Science(all)
- Environmental Science (miscellaneous)
- Immunology and Microbiology(all)
- Microbiology
- Agricultural and Biological Sciences(all)
- Ecology, Evolution, Behavior and Systematics
- Medicine(all)
- Infectious Diseases
Sustainable Development Goals
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In: Frontiers in Fungal Biology, Vol. 2, 777474, 03.01.2022.
Research output: Contribution to journal › Article › Research › peer review
}
TY - JOUR
T1 - Biosynthesis of Antibacterial Iron-Chelating Tropolones in Aspergillus nidulans as Response to Glycopeptide-Producing Streptomycetes
AU - Gerke, Jennifer
AU - Köhler, Anna M.
AU - Wennrich, Jan Peer
AU - Große, Verena
AU - Shao, Lulu
AU - Heinrich, Antje K.
AU - Bode, Helge B.
AU - Chen, Wanping
AU - Surup, Frank
AU - Braus, Gerhard H.
N1 - Funding Information: Funding was provided by the German Research Council to GB (DFG grant BR 1502/19-1). We acknowledge support by the Open Access Publication Funds of the University of Göttingen.
PY - 2022/1/3
Y1 - 2022/1/3
N2 - The soil microbiome comprises numerous filamentous fungi and bacteria that mutually react and challenge each other by the production of bioactive secondary metabolites. Herein, we show in liquid co-cultures that the presence of filamentous Streptomycetes producing antifungal glycopeptide antibiotics induces the production of the antibacterial and iron-chelating tropolones anhydrosepedonin (1) and antibiotic C (2) in the mold Aspergillus nidulans. Additionally, the biosynthesis of the related polyketide tripyrnidone (5) was induced, whose novel tricyclic scaffold we elucidated by NMR and HRESIMS data. The corresponding biosynthetic polyketide synthase-encoding gene cluster responsible for the production of these compounds was identified. The tropolones as well as tripyrnidone (5) are produced by genes that belong to the broad reservoir of the fungal genome for the synthesis of different secondary metabolites, which are usually silenced under standard laboratory conditions. These molecules might be part of the bacterium-fungus competition in the complex soil environment, with the bacterial glycopeptide antibiotic as specific environmental trigger for fungal induction of this cluster.
AB - The soil microbiome comprises numerous filamentous fungi and bacteria that mutually react and challenge each other by the production of bioactive secondary metabolites. Herein, we show in liquid co-cultures that the presence of filamentous Streptomycetes producing antifungal glycopeptide antibiotics induces the production of the antibacterial and iron-chelating tropolones anhydrosepedonin (1) and antibiotic C (2) in the mold Aspergillus nidulans. Additionally, the biosynthesis of the related polyketide tripyrnidone (5) was induced, whose novel tricyclic scaffold we elucidated by NMR and HRESIMS data. The corresponding biosynthetic polyketide synthase-encoding gene cluster responsible for the production of these compounds was identified. The tropolones as well as tripyrnidone (5) are produced by genes that belong to the broad reservoir of the fungal genome for the synthesis of different secondary metabolites, which are usually silenced under standard laboratory conditions. These molecules might be part of the bacterium-fungus competition in the complex soil environment, with the bacterial glycopeptide antibiotic as specific environmental trigger for fungal induction of this cluster.
KW - Aspergillus nidulans
KW - fungal-bacterial co-cultivation
KW - glycopeptide antibiotics
KW - Streptomyces
KW - structure elucidation
KW - tropolones
UR - http://www.scopus.com/inward/record.url?scp=85138496911&partnerID=8YFLogxK
U2 - 10.3389/ffunb.2021.777474
DO - 10.3389/ffunb.2021.777474
M3 - Article
AN - SCOPUS:85138496911
VL - 2
JO - Frontiers in Fungal Biology
JF - Frontiers in Fungal Biology
SN - 2673-6128
M1 - 777474
ER -