Details
| Original language | English |
|---|---|
| Pages (from-to) | 25-33 |
| Number of pages | 9 |
| Journal | VIROLOGY |
| Volume | 518 |
| Early online date | 30 Jan 2018 |
| Publication status | Published - May 2018 |
Abstract
Two members of the Benyviridae family and genus Benyvirus, Beet soil-borne mosaic virus (BSBMV) and Beet necrotic yellow vein virus (BNYVV), possess identical genome organization, host range and high sequence similarity; they infect Beta vulgaris with variable symptom expression. In the US, mixed infections are described with limited information about viral interactions. Vectors suitable for agroinoculation of all genome components of both viruses were constructed by isothermal in vitro recombination. All 35S promoter-driven cDNA clones allowed production of recombinant viruses competent for Nicotiana benthamiana and Beta macrocarpa systemic infection and Polymyxa betae transmission and were compared to available BNYVV B-type clone. BNYVV and BSBMV RNA1 + 2 reassortants were viable and spread long-distance in N. benthamiana with symptoms dependent on the BNYVV type. Small genomic RNAs were exchangeable and systemically infected B. macrocarpa. These infectious clones represent a powerful tool for the identification of specific molecular host-pathogen determinants.
Keywords
- Beet necrotic yellow vein virus, Beet soil-borne mosaic virus, Benyviridae, Gibson assembly, Infectious cDNA full-length clones, Polymyxa betae, Reassortment
ASJC Scopus subject areas
- Immunology and Microbiology(all)
- Virology
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In: VIROLOGY, Vol. 518, 05.2018, p. 25-33.
Research output: Contribution to journal › Article › Research › peer review
}
TY - JOUR
T1 - Biological properties of Beet soil-borne mosaic virus and Beet necrotic yellow vein virus cDNA clones produced by isothermal in vitro recombination: Insights for reassortant appearance
AU - Laufer, Marlene
AU - Mohammad, Hamza
AU - Maiss, Edgar
AU - Richert-Pöggeler, Katja
AU - Dall'Ara, Mattia
AU - Ratti, Claudio
AU - Gilmer, David
AU - Liebe, Sebastian
AU - Varrelmann, Mark
N1 - Funding information: This research was supported in part by the Deutsche Forschungsgemeinschaft (DFG) grant VA 202/7-1 .
PY - 2018/5
Y1 - 2018/5
N2 - Two members of the Benyviridae family and genus Benyvirus, Beet soil-borne mosaic virus (BSBMV) and Beet necrotic yellow vein virus (BNYVV), possess identical genome organization, host range and high sequence similarity; they infect Beta vulgaris with variable symptom expression. In the US, mixed infections are described with limited information about viral interactions. Vectors suitable for agroinoculation of all genome components of both viruses were constructed by isothermal in vitro recombination. All 35S promoter-driven cDNA clones allowed production of recombinant viruses competent for Nicotiana benthamiana and Beta macrocarpa systemic infection and Polymyxa betae transmission and were compared to available BNYVV B-type clone. BNYVV and BSBMV RNA1 + 2 reassortants were viable and spread long-distance in N. benthamiana with symptoms dependent on the BNYVV type. Small genomic RNAs were exchangeable and systemically infected B. macrocarpa. These infectious clones represent a powerful tool for the identification of specific molecular host-pathogen determinants.
AB - Two members of the Benyviridae family and genus Benyvirus, Beet soil-borne mosaic virus (BSBMV) and Beet necrotic yellow vein virus (BNYVV), possess identical genome organization, host range and high sequence similarity; they infect Beta vulgaris with variable symptom expression. In the US, mixed infections are described with limited information about viral interactions. Vectors suitable for agroinoculation of all genome components of both viruses were constructed by isothermal in vitro recombination. All 35S promoter-driven cDNA clones allowed production of recombinant viruses competent for Nicotiana benthamiana and Beta macrocarpa systemic infection and Polymyxa betae transmission and were compared to available BNYVV B-type clone. BNYVV and BSBMV RNA1 + 2 reassortants were viable and spread long-distance in N. benthamiana with symptoms dependent on the BNYVV type. Small genomic RNAs were exchangeable and systemically infected B. macrocarpa. These infectious clones represent a powerful tool for the identification of specific molecular host-pathogen determinants.
KW - Beet necrotic yellow vein virus
KW - Beet soil-borne mosaic virus
KW - Benyviridae
KW - Gibson assembly
KW - Infectious cDNA full-length clones
KW - Polymyxa betae
KW - Reassortment
UR - http://www.scopus.com/inward/record.url?scp=85041849117&partnerID=8YFLogxK
U2 - 10.1016/j.virol.2018.01.029
DO - 10.1016/j.virol.2018.01.029
M3 - Article
C2 - 29453056
AN - SCOPUS:85041849117
VL - 518
SP - 25
EP - 33
JO - VIROLOGY
JF - VIROLOGY
SN - 0042-6822
ER -