Details
| Original language | English |
|---|---|
| Pages (from-to) | 445-449 |
| Number of pages | 5 |
| Journal | Journal of General Virology |
| Volume | 87 |
| Issue number | 2 |
| Publication status | Published - 1 Feb 2006 |
Abstract
A German isolate of Beet mild yellowing virus (BMYV-IPP) was used for RT-PCR-based construction of the first infectious full-length cDNA clone of the virus (BMYVfl). The complete genomic sequence was determined and displayed high similarity to the French isolate BMYV-21TB. The host range of BMYVfl was examined by agroinoculation and aphid transmission. Both methods lead to systemic infections in Beta vulgaris, Nicotiana benthamiana, N. clevelandii, N. hesperis, Capsella bursa-pastoris and Lamium purpureum. Immunological investigation by tissue-print immunoassay (TPIA) of agroinoculated plant tissues revealed only local infections restricted to the agroinoculated mesophyll tissues in some plant species. In Nicotiana glutinosa and N. edwardsonii, BMYV was not found in either the agroinoculated tissue or distant tissues by TPIA. So far, BMYVfl agroinoculation did not extend or confine the BMYV host range known from aphid transmission experiments but it did describe new local hosts for BMYV.
ASJC Scopus subject areas
- Immunology and Microbiology(all)
- Virology
Cite this
- Standard
- Harvard
- Apa
- Vancouver
- BibTeX
- RIS
In: Journal of General Virology, Vol. 87, No. 2, 01.02.2006, p. 445-449.
Research output: Contribution to journal › Article › Research › peer review
}
TY - JOUR
T1 - Biological properties of Beet mild yellowing virus derived from a full-length cDNA clone
AU - Stephan, Dirk
AU - Maiss, Edgar
PY - 2006/2/1
Y1 - 2006/2/1
N2 - A German isolate of Beet mild yellowing virus (BMYV-IPP) was used for RT-PCR-based construction of the first infectious full-length cDNA clone of the virus (BMYVfl). The complete genomic sequence was determined and displayed high similarity to the French isolate BMYV-21TB. The host range of BMYVfl was examined by agroinoculation and aphid transmission. Both methods lead to systemic infections in Beta vulgaris, Nicotiana benthamiana, N. clevelandii, N. hesperis, Capsella bursa-pastoris and Lamium purpureum. Immunological investigation by tissue-print immunoassay (TPIA) of agroinoculated plant tissues revealed only local infections restricted to the agroinoculated mesophyll tissues in some plant species. In Nicotiana glutinosa and N. edwardsonii, BMYV was not found in either the agroinoculated tissue or distant tissues by TPIA. So far, BMYVfl agroinoculation did not extend or confine the BMYV host range known from aphid transmission experiments but it did describe new local hosts for BMYV.
AB - A German isolate of Beet mild yellowing virus (BMYV-IPP) was used for RT-PCR-based construction of the first infectious full-length cDNA clone of the virus (BMYVfl). The complete genomic sequence was determined and displayed high similarity to the French isolate BMYV-21TB. The host range of BMYVfl was examined by agroinoculation and aphid transmission. Both methods lead to systemic infections in Beta vulgaris, Nicotiana benthamiana, N. clevelandii, N. hesperis, Capsella bursa-pastoris and Lamium purpureum. Immunological investigation by tissue-print immunoassay (TPIA) of agroinoculated plant tissues revealed only local infections restricted to the agroinoculated mesophyll tissues in some plant species. In Nicotiana glutinosa and N. edwardsonii, BMYV was not found in either the agroinoculated tissue or distant tissues by TPIA. So far, BMYVfl agroinoculation did not extend or confine the BMYV host range known from aphid transmission experiments but it did describe new local hosts for BMYV.
UR - http://www.scopus.com/inward/record.url?scp=31644434212&partnerID=8YFLogxK
U2 - 10.1099/vir.0.81565-0
DO - 10.1099/vir.0.81565-0
M3 - Article
C2 - 16432033
AN - SCOPUS:31644434212
VL - 87
SP - 445
EP - 449
JO - Journal of General Virology
JF - Journal of General Virology
SN - 0022-1317
IS - 2
ER -