Details
| Original language | English |
|---|---|
| Pages (from-to) | 3089-3098 |
| Number of pages | 10 |
| Journal | BIOMACROMOLECULES |
| Volume | 18 |
| Issue number | 10 |
| Publication status | Published - 9 Oct 2017 |
Abstract
We report the chemical vapor deposition (CVD) of dual-functional polymer films for the specific and orthogonal immobilization of two biomolecules (notch ligand delta-like 1 (DLL1) and an RGD-peptide) that govern the fate of hematopoietic stem and progenitor cells. The composition of the CVD polymer and thus the biomolecule ratio can be tailored to investigate and optimize the influence of the relative surface concentrations of biomolecules on stem cell behavior. Prior to cell experiments, all surfaces were characterized by infrared reflection adsorption spectroscopy, time-of-flight secondary ion mass spectrometry, and X-ray photoelectron spectroscopy to confirm the presence of both biomolecules. In a proof-of-principle stem cell culture study, we show that all polymer surfaces are cytocompatible and that the proliferation of the hematopoietic stem and progenitor cells is predominantly influenced by the surface concentration of immobilized DLL1.
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In: BIOMACROMOLECULES, Vol. 18, No. 10, 09.10.2017, p. 3089-3098.
Research output: Contribution to journal › Article › Research › peer review
}
TY - JOUR
T1 - Bioinstructive Coatings for Hematopoietic Stem Cell Expansion Based on Chemical Vapor Deposition Copolymerization
AU - Winkler, Anna-Lena
AU - Koenig, Meike
AU - Welle, Alexander
AU - Trouillet, Vanessa
AU - Kratzer, Domenic
AU - Hussal, Christoph
AU - Lahann, Joerg
AU - Lee-Thedieck, Cornelia
N1 - Funding information: The project was funded by the BMBF NanoMatFutur Program (FKZ 13N12968). A.W., M.K., C.H., J.L., and C.L.T. acknowledge support by the program “Biointerfaces in Technology and Medicine” of the Helmholtz Association. C.H. and J.L. thank the German Research Foundation (DFG) for financial support within the frame of the collaborative research center SFB 1176 (Project B3). The authors thank Saskia Kraus for excellent technical assistance. The K-Alpha+ instrument was financially supported by the Federal Ministry of Economics and Technology.
PY - 2017/10/9
Y1 - 2017/10/9
N2 - We report the chemical vapor deposition (CVD) of dual-functional polymer films for the specific and orthogonal immobilization of two biomolecules (notch ligand delta-like 1 (DLL1) and an RGD-peptide) that govern the fate of hematopoietic stem and progenitor cells. The composition of the CVD polymer and thus the biomolecule ratio can be tailored to investigate and optimize the influence of the relative surface concentrations of biomolecules on stem cell behavior. Prior to cell experiments, all surfaces were characterized by infrared reflection adsorption spectroscopy, time-of-flight secondary ion mass spectrometry, and X-ray photoelectron spectroscopy to confirm the presence of both biomolecules. In a proof-of-principle stem cell culture study, we show that all polymer surfaces are cytocompatible and that the proliferation of the hematopoietic stem and progenitor cells is predominantly influenced by the surface concentration of immobilized DLL1.
AB - We report the chemical vapor deposition (CVD) of dual-functional polymer films for the specific and orthogonal immobilization of two biomolecules (notch ligand delta-like 1 (DLL1) and an RGD-peptide) that govern the fate of hematopoietic stem and progenitor cells. The composition of the CVD polymer and thus the biomolecule ratio can be tailored to investigate and optimize the influence of the relative surface concentrations of biomolecules on stem cell behavior. Prior to cell experiments, all surfaces were characterized by infrared reflection adsorption spectroscopy, time-of-flight secondary ion mass spectrometry, and X-ray photoelectron spectroscopy to confirm the presence of both biomolecules. In a proof-of-principle stem cell culture study, we show that all polymer surfaces are cytocompatible and that the proliferation of the hematopoietic stem and progenitor cells is predominantly influenced by the surface concentration of immobilized DLL1.
UR - http://www.scopus.com/inward/record.url?scp=85031292503&partnerID=8YFLogxK
U2 - 10.1021/acs.biomac.7b00743
DO - 10.1021/acs.biomac.7b00743
M3 - Article
VL - 18
SP - 3089
EP - 3098
JO - BIOMACROMOLECULES
JF - BIOMACROMOLECULES
SN - 1525-7797
IS - 10
ER -