Details
| Original language | English |
|---|---|
| Article number | 183 |
| Pages (from-to) | 1-27 |
| Number of pages | 27 |
| Journal | Polymers |
| Volume | 12 |
| Issue number | 1 |
| Publication status | Published - 10 Jan 2020 |
Abstract
In this work, a method to prepare hybrid amphiphilic block copolymers consisting of biocompatible synthetic glycopolymer with non-degradable backbone and biodegradable poly(amino acid) (PAA) was developed. The glycopolymer, poly(2-deoxy-2-methacrylamido-D-glucose) (PMAG), was synthesized via reversible addition-fragmentation chain transfer (RAFT) polymerization. Two methods for modifying the terminal dithiobenzoate-group of PMAG was investigated to obtain the macroinitiator bearing a primary aliphatic amino group, which is required for ring-opening polymerization of N-carboxyanhydrides of hydrophobic α-amino acids. The synthesized amphiphilic block copolymers were carefully analyzed using a set of different physico-chemical methods to establish their composition and molecular weight. The developed amphiphilic copolymers tended to self-assemble in nanoparticles of different morphology that depended on the nature of the hydrophobic amino acid present in the copolymer. The hydrodynamic diameter, morphology, and cytotoxicity of polymer particles based on PMAG-b-PAA were evaluated using dynamic light scattering (DLS) and transmission electron microscopy (TEM), as well as CellTiter-Blue (CTB) assay, respectively. The redox-responsive properties of nanoparticles were evaluated in the presence of glutathione taken at different concentrations. Moreover, the encapsulation of paclitaxel into PMAG-b-PAA particles and their cytotoxicity on human lung carcinoma cells (A549) and human breast adenocarcinoma cells (MCF-7) were studied.
Keywords
- Blend, Nanocomposites, Physical properties, Poly(vinylidene fluoride), Polyamide 6, Drug delivery systems, Amphiphilic block copolymers, Poly(amino acids), Nanoparticles, Modification of terminal groups, Paclitaxel, Redox-responsive systems, Synthetic glycopolymers
ASJC Scopus subject areas
- Chemistry(all)
- General Chemistry
- Materials Science(all)
- Polymers and Plastics
Cite this
- Standard
- Harvard
- Apa
- Vancouver
- BibTeX
- RIS
In: Polymers, Vol. 12, No. 1, 183, 10.01.2020, p. 1-27.
Research output: Contribution to journal › Article › Research › peer review
}
TY - JOUR
T1 - Bio-inspired amphiphilic block-copolymers based on synthetic glycopolymer and poly (amino acid) as potential drug delivery systems
AU - Levit, Mariia
AU - Zashikhina, Natalia
AU - Vdovchenko, Alena
AU - Dobrodumov, Anatoliy
AU - Zakharova, Natalya
AU - Kashina, Anna
AU - Rühl, Eckart
AU - Lavrentieva, Antonina
AU - Scheper, Thomas
AU - Tennikova, Tatiana
AU - Korzhikova-Vlakh, Evgenia
N1 - Funding information: The research was supported financially by the Russian Foundation for Basic Research (RFBR, research project no. 18-33-01018) and the G-RISC scholarship program for A. Vdovchenko and N. Zashikhina (projects #B-2017a-2 and #C-2018b-5, respectively). ER acknowledges DFG for funding (grant no. RU 420/12-1).
PY - 2020/1/10
Y1 - 2020/1/10
N2 - In this work, a method to prepare hybrid amphiphilic block copolymers consisting of biocompatible synthetic glycopolymer with non-degradable backbone and biodegradable poly(amino acid) (PAA) was developed. The glycopolymer, poly(2-deoxy-2-methacrylamido-D-glucose) (PMAG), was synthesized via reversible addition-fragmentation chain transfer (RAFT) polymerization. Two methods for modifying the terminal dithiobenzoate-group of PMAG was investigated to obtain the macroinitiator bearing a primary aliphatic amino group, which is required for ring-opening polymerization of N-carboxyanhydrides of hydrophobic α-amino acids. The synthesized amphiphilic block copolymers were carefully analyzed using a set of different physico-chemical methods to establish their composition and molecular weight. The developed amphiphilic copolymers tended to self-assemble in nanoparticles of different morphology that depended on the nature of the hydrophobic amino acid present in the copolymer. The hydrodynamic diameter, morphology, and cytotoxicity of polymer particles based on PMAG-b-PAA were evaluated using dynamic light scattering (DLS) and transmission electron microscopy (TEM), as well as CellTiter-Blue (CTB) assay, respectively. The redox-responsive properties of nanoparticles were evaluated in the presence of glutathione taken at different concentrations. Moreover, the encapsulation of paclitaxel into PMAG-b-PAA particles and their cytotoxicity on human lung carcinoma cells (A549) and human breast adenocarcinoma cells (MCF-7) were studied.
AB - In this work, a method to prepare hybrid amphiphilic block copolymers consisting of biocompatible synthetic glycopolymer with non-degradable backbone and biodegradable poly(amino acid) (PAA) was developed. The glycopolymer, poly(2-deoxy-2-methacrylamido-D-glucose) (PMAG), was synthesized via reversible addition-fragmentation chain transfer (RAFT) polymerization. Two methods for modifying the terminal dithiobenzoate-group of PMAG was investigated to obtain the macroinitiator bearing a primary aliphatic amino group, which is required for ring-opening polymerization of N-carboxyanhydrides of hydrophobic α-amino acids. The synthesized amphiphilic block copolymers were carefully analyzed using a set of different physico-chemical methods to establish their composition and molecular weight. The developed amphiphilic copolymers tended to self-assemble in nanoparticles of different morphology that depended on the nature of the hydrophobic amino acid present in the copolymer. The hydrodynamic diameter, morphology, and cytotoxicity of polymer particles based on PMAG-b-PAA were evaluated using dynamic light scattering (DLS) and transmission electron microscopy (TEM), as well as CellTiter-Blue (CTB) assay, respectively. The redox-responsive properties of nanoparticles were evaluated in the presence of glutathione taken at different concentrations. Moreover, the encapsulation of paclitaxel into PMAG-b-PAA particles and their cytotoxicity on human lung carcinoma cells (A549) and human breast adenocarcinoma cells (MCF-7) were studied.
KW - Blend
KW - Nanocomposites
KW - Physical properties
KW - Poly(vinylidene fluoride)
KW - Polyamide 6
KW - Drug delivery systems
KW - Amphiphilic block copolymers
KW - Poly(amino acids)
KW - Nanoparticles
KW - Modification of terminal groups
KW - Paclitaxel
KW - Redox-responsive systems
KW - Synthetic glycopolymers
UR - http://www.scopus.com/inward/record.url?scp=85082653219&partnerID=8YFLogxK
U2 - 10.3390/polym12010183
DO - 10.3390/polym12010183
M3 - Article
C2 - 32284516
VL - 12
SP - 1
EP - 27
JO - Polymers
JF - Polymers
SN - 2073-4360
IS - 1
M1 - 183
ER -