Details
| Original language | English |
|---|---|
| Pages (from-to) | 96-103 |
| Number of pages | 8 |
| Journal | Colloids and Surfaces B: Biointerfaces |
| Volume | 114 |
| Publication status | Published - 4 Oct 2013 |
Abstract
We describe here the synthesis, characterization, bioconjugation, and application of water-soluble thioglycolic acid TGA-capped CdTe/CdS quantum dots (TGA-QDs) for targeted cellular imaging. Anti-human epidermal growth factor receptor 2 (HER2) antibodies were conjugated to TGA-QDs to target HER2-overexpressing cancer cells. TGA-QDs and TGA-QDs/anti-HER2 bioconjugates were characterized by fluorescence and UV-Vis spectroscopy, X-ray diffraction (XRD), hydrodynamic sizing, electron microscopy, and gel electrophoresis. TGA-QDs and TGA-QDs/anti-HER2 were incubated with cells to examine cytotoxicity, targeting efficiency, and cellular localization. The cytotoxicity of particles was measured using an MTT assay and the no observable adverse effect concentration (NOAEC), 50% inhibitory concentration (IC50), and total lethal concentration (TLC) were calculated. To evaluate localization and targeting efficiency of TGA-QDs with or without antibodies, fluorescence microscopy and flow cytometry were performed. Our results indicate that antibody-conjugated TGA-QDs are well-suited for targeted cellular imaging studies.
Keywords
- Anti-HER2, Bioconjugation, Cell specific targeting, Imaging, Quantum dots
ASJC Scopus subject areas
- Biochemistry, Genetics and Molecular Biology(all)
- Biotechnology
- Physics and Astronomy(all)
- Surfaces and Interfaces
- Chemistry(all)
- Physical and Theoretical Chemistry
- Chemical Engineering(all)
- Colloid and Surface Chemistry
Sustainable Development Goals
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In: Colloids and Surfaces B: Biointerfaces, Vol. 114, 04.10.2013, p. 96-103.
Research output: Contribution to journal › Article › Research › peer review
}
TY - JOUR
T1 - Biofunctional quantum dots as fluorescence probe for cell-specific targeting
AU - Ag, Didem
AU - Bongartz, Rebecca
AU - Dogan, Leyla Eral
AU - Seleci, Muharrem
AU - Walter, Johanna G.
AU - Demirkol, Dilek Odaci
AU - Stahl, Frank
AU - Ozcelik, Serdar
AU - Timur, Suna
AU - Scheper, Thomas
N1 - Funding information: This work is supported by Scientific and Technological Research Council of Turkey (TUBITAK, project number 109T573 ) and Federal Ministry of Education and Research (BMBF, project number 01DL12013 ). Also it was partially funded by Ege University Scientific Research Project ( 2012/FEN/0071 ). We are grateful to Michael Meyer (Gottfried Wilhelm Leibniz University of Hannover, Institute for Technical Chemistry) for his knowledge and time spent carrying out flow cytometry experiments. In addition our thanks go to F. B. Barlas (Ege University, Faculty of Science, Biochemistry department) for taking fluorescence microscope images.
PY - 2013/10/4
Y1 - 2013/10/4
N2 - We describe here the synthesis, characterization, bioconjugation, and application of water-soluble thioglycolic acid TGA-capped CdTe/CdS quantum dots (TGA-QDs) for targeted cellular imaging. Anti-human epidermal growth factor receptor 2 (HER2) antibodies were conjugated to TGA-QDs to target HER2-overexpressing cancer cells. TGA-QDs and TGA-QDs/anti-HER2 bioconjugates were characterized by fluorescence and UV-Vis spectroscopy, X-ray diffraction (XRD), hydrodynamic sizing, electron microscopy, and gel electrophoresis. TGA-QDs and TGA-QDs/anti-HER2 were incubated with cells to examine cytotoxicity, targeting efficiency, and cellular localization. The cytotoxicity of particles was measured using an MTT assay and the no observable adverse effect concentration (NOAEC), 50% inhibitory concentration (IC50), and total lethal concentration (TLC) were calculated. To evaluate localization and targeting efficiency of TGA-QDs with or without antibodies, fluorescence microscopy and flow cytometry were performed. Our results indicate that antibody-conjugated TGA-QDs are well-suited for targeted cellular imaging studies.
AB - We describe here the synthesis, characterization, bioconjugation, and application of water-soluble thioglycolic acid TGA-capped CdTe/CdS quantum dots (TGA-QDs) for targeted cellular imaging. Anti-human epidermal growth factor receptor 2 (HER2) antibodies were conjugated to TGA-QDs to target HER2-overexpressing cancer cells. TGA-QDs and TGA-QDs/anti-HER2 bioconjugates were characterized by fluorescence and UV-Vis spectroscopy, X-ray diffraction (XRD), hydrodynamic sizing, electron microscopy, and gel electrophoresis. TGA-QDs and TGA-QDs/anti-HER2 were incubated with cells to examine cytotoxicity, targeting efficiency, and cellular localization. The cytotoxicity of particles was measured using an MTT assay and the no observable adverse effect concentration (NOAEC), 50% inhibitory concentration (IC50), and total lethal concentration (TLC) were calculated. To evaluate localization and targeting efficiency of TGA-QDs with or without antibodies, fluorescence microscopy and flow cytometry were performed. Our results indicate that antibody-conjugated TGA-QDs are well-suited for targeted cellular imaging studies.
KW - Anti-HER2
KW - Bioconjugation
KW - Cell specific targeting
KW - Imaging
KW - Quantum dots
UR - http://www.scopus.com/inward/record.url?scp=84886626978&partnerID=8YFLogxK
U2 - 10.1016/j.colsurfb.2013.09.033
DO - 10.1016/j.colsurfb.2013.09.033
M3 - Article
C2 - 24176888
AN - SCOPUS:84886626978
VL - 114
SP - 96
EP - 103
JO - Colloids and Surfaces B: Biointerfaces
JF - Colloids and Surfaces B: Biointerfaces
SN - 0927-7765
ER -