Details
| Original language | English |
|---|---|
| Pages (from-to) | 6103-6115 |
| Number of pages | 13 |
| Journal | Applied Microbiology and Biotechnology |
| Volume | 105 |
| Issue number | 14-15 |
| Early online date | 2 Aug 2021 |
| Publication status | Published - Aug 2021 |
Abstract
Keywords
- 16S rRNA microbiome analysis, Hyporheic zone, Metoprolol, Micropollutant transformation
ASJC Scopus subject areas
- Biochemistry, Genetics and Molecular Biology(all)
- Biotechnology
- Immunology and Microbiology(all)
- Applied Microbiology and Biotechnology
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In: Applied Microbiology and Biotechnology, Vol. 105, No. 14-15, 08.2021, p. 6103-6115.
Research output: Contribution to journal › Article › Research › peer review
}
TY - JOUR
T1 - Biodegradation of metoprolol in oxic and anoxic hyporheic zone sediments: unexpected effects on microbial communities
AU - Rutere, Cyrus
AU - Posselt, Malte
AU - Ho, Adrian
AU - Horn, Marcus A.
N1 - Funding Information: Open access funding enabled and organized by Projekt DEAL. This study was funded by the European Union’s Horizon 2020 research and innovation program under the Marie Sklodowska-Curie grant agreement no. 641939. Partial funding was also provided by the Leibniz University Hannover, Germany.
PY - 2021/8
Y1 - 2021/8
N2 - Metoprolol is widely used as a beta-blocker and considered an emerging contaminant of environmental concern due to pseudo persistence in wastewater effluents that poses a potential ecotoxicological threat to aquatic ecosystems. Microbial removal of metoprolol in the redox-delineated hyporheic zone (HZ) was investigated using streambed sediments supplemented with 15 or 150 μM metoprolol in a laboratory microcosm incubation under oxic and anoxic conditions. Metoprolol disappeared from the aqueous phase under oxic and anoxic conditions within 65 and 72 days, respectively. Metoprolol was refed twice after initial depletion resulting in accelerated disappearance under both conditions. Metoprolol disappearance was marginal in sterile control microcosms with autoclaved sediment. Metoprolol was transformed mainly to metoprolol acid in oxic microcosms, while metoprolol acid and α-hydroxymetoprolol were formed in anoxic microcosms. Transformation products were transient and disappeared within 30 days under both conditions. Effects of metoprolol on the HZ bacterial community were evaluated using DNA- and RNA-based time-resolved amplicon Illumina MiSeq sequencing targeting the 16S rRNA gene and 16S rRNA, respectively, and were prominent on 16S rRNA rather than 16S rRNA gene level suggesting moderate metoprolol-induced activity-level changes. A positive impact of metoprolol on Sphingomonadaceae and Enterobacteriaceae under oxic and anoxic conditions, respectively, was observed. Nitrifiers were impaired by metoprolol under oxic and anoxic conditions. Collectively, our findings revealed high metoprolol biodegradation potentials in the hyporheic zone under contrasting redox conditions associated with changes in the active microbial communities, thus contributing to the attenuation of micropollutants.
AB - Metoprolol is widely used as a beta-blocker and considered an emerging contaminant of environmental concern due to pseudo persistence in wastewater effluents that poses a potential ecotoxicological threat to aquatic ecosystems. Microbial removal of metoprolol in the redox-delineated hyporheic zone (HZ) was investigated using streambed sediments supplemented with 15 or 150 μM metoprolol in a laboratory microcosm incubation under oxic and anoxic conditions. Metoprolol disappeared from the aqueous phase under oxic and anoxic conditions within 65 and 72 days, respectively. Metoprolol was refed twice after initial depletion resulting in accelerated disappearance under both conditions. Metoprolol disappearance was marginal in sterile control microcosms with autoclaved sediment. Metoprolol was transformed mainly to metoprolol acid in oxic microcosms, while metoprolol acid and α-hydroxymetoprolol were formed in anoxic microcosms. Transformation products were transient and disappeared within 30 days under both conditions. Effects of metoprolol on the HZ bacterial community were evaluated using DNA- and RNA-based time-resolved amplicon Illumina MiSeq sequencing targeting the 16S rRNA gene and 16S rRNA, respectively, and were prominent on 16S rRNA rather than 16S rRNA gene level suggesting moderate metoprolol-induced activity-level changes. A positive impact of metoprolol on Sphingomonadaceae and Enterobacteriaceae under oxic and anoxic conditions, respectively, was observed. Nitrifiers were impaired by metoprolol under oxic and anoxic conditions. Collectively, our findings revealed high metoprolol biodegradation potentials in the hyporheic zone under contrasting redox conditions associated with changes in the active microbial communities, thus contributing to the attenuation of micropollutants.
KW - 16S rRNA microbiome analysis
KW - Hyporheic zone
KW - Metoprolol
KW - Micropollutant transformation
UR - http://www.scopus.com/inward/record.url?scp=85111795197&partnerID=8YFLogxK
U2 - 10.1007/s00253-021-11466-w
DO - 10.1007/s00253-021-11466-w
M3 - Article
VL - 105
SP - 6103
EP - 6115
JO - Applied Microbiology and Biotechnology
JF - Applied Microbiology and Biotechnology
SN - 1432-0614
IS - 14-15
ER -