Details
| Original language | English |
|---|---|
| Pages (from-to) | 1593-1601 |
| Number of pages | 9 |
| Journal | Journal of experimental botany |
| Volume | 45 |
| Issue number | 11 |
| Publication status | Published - 1 Nov 1994 |
Abstract
A monoclonal antibody of IgM-type (TIM-11B2) was screened from a hybridoma library. The antibody recognizes a 40 kDa glycoprotein, p40, with high specificity. This protein was detected in all plant species examined so far and was found to be located both solubly and ionically-bound within the primary cell wall.The strongest immunobiochemical signals of p40 were found in tissues undergoing elongation growth, whereas in other tissues only a faint signal could be detected. Those included the non-elongating parts of different seedlings, such as the apical part of monocot primary leaves or the leaves of dicots grown in light. Inhibition of pea epicotyl growth by white light irradiation resulted in a strong decrease of the immunostain signal. On the other hand, induction of rapid coleoptile growth in rice seedlings induced by submergence resulted in a strong increase of the immunobiochemical signal of p40. Time-course studies on the expression of p40 during protoplast regeneration revealed that p40 is apparently not involved in cell wall formation. The hypothesis that p40 is characteristic for tissues with the ability for elongation growth is discussed.Comparison of biochemical data and location of p40 with proteins described up to now indicate that this glycoprotein has not been characterized before.
Keywords
- Cell wall protein, Elongation growth, Monoclonal antibody
ASJC Scopus subject areas
- Biochemistry, Genetics and Molecular Biology(all)
- Physiology
- Agricultural and Biological Sciences(all)
- Plant Science
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In: Journal of experimental botany, Vol. 45, No. 11, 01.11.1994, p. 1593-1601.
Research output: Contribution to journal › Article › Research › peer review
}
TY - JOUR
T1 - Biochemical properties of a novel cell wall protein associated with elongation growth in higher plants
AU - Reinard, Thomas
AU - Sprunck, Stefanie
AU - Altherr, Sandra
AU - Jacobsen, Hans Jörg
N1 - Funding Information: This work was supported by grants from Graduierten FSrderung NRW to T.R. and from Bundesministerium fDr Forschung und Technologie to H.-J.J. (BE021-0318969C-0318969C9).
PY - 1994/11/1
Y1 - 1994/11/1
N2 - A monoclonal antibody of IgM-type (TIM-11B2) was screened from a hybridoma library. The antibody recognizes a 40 kDa glycoprotein, p40, with high specificity. This protein was detected in all plant species examined so far and was found to be located both solubly and ionically-bound within the primary cell wall.The strongest immunobiochemical signals of p40 were found in tissues undergoing elongation growth, whereas in other tissues only a faint signal could be detected. Those included the non-elongating parts of different seedlings, such as the apical part of monocot primary leaves or the leaves of dicots grown in light. Inhibition of pea epicotyl growth by white light irradiation resulted in a strong decrease of the immunostain signal. On the other hand, induction of rapid coleoptile growth in rice seedlings induced by submergence resulted in a strong increase of the immunobiochemical signal of p40. Time-course studies on the expression of p40 during protoplast regeneration revealed that p40 is apparently not involved in cell wall formation. The hypothesis that p40 is characteristic for tissues with the ability for elongation growth is discussed.Comparison of biochemical data and location of p40 with proteins described up to now indicate that this glycoprotein has not been characterized before.
AB - A monoclonal antibody of IgM-type (TIM-11B2) was screened from a hybridoma library. The antibody recognizes a 40 kDa glycoprotein, p40, with high specificity. This protein was detected in all plant species examined so far and was found to be located both solubly and ionically-bound within the primary cell wall.The strongest immunobiochemical signals of p40 were found in tissues undergoing elongation growth, whereas in other tissues only a faint signal could be detected. Those included the non-elongating parts of different seedlings, such as the apical part of monocot primary leaves or the leaves of dicots grown in light. Inhibition of pea epicotyl growth by white light irradiation resulted in a strong decrease of the immunostain signal. On the other hand, induction of rapid coleoptile growth in rice seedlings induced by submergence resulted in a strong increase of the immunobiochemical signal of p40. Time-course studies on the expression of p40 during protoplast regeneration revealed that p40 is apparently not involved in cell wall formation. The hypothesis that p40 is characteristic for tissues with the ability for elongation growth is discussed.Comparison of biochemical data and location of p40 with proteins described up to now indicate that this glycoprotein has not been characterized before.
KW - Cell wall protein
KW - Elongation growth
KW - Monoclonal antibody
UR - http://www.scopus.com/inward/record.url?scp=7544247983&partnerID=8YFLogxK
U2 - 10.1093/jxb/45.11.1593
DO - 10.1093/jxb/45.11.1593
M3 - Article
AN - SCOPUS:7544247983
VL - 45
SP - 1593
EP - 1601
JO - Journal of experimental botany
JF - Journal of experimental botany
SN - 0022-0957
IS - 11
ER -