Details
| Original language | English |
|---|---|
| Pages (from-to) | 119-128 |
| Number of pages | 10 |
| Journal | Genes and Development |
| Volume | 11 |
| Issue number | 1 |
| Publication status | Published - 1 Jan 1997 |
| Externally published | Yes |
Abstract
Development of genetic competence in Bacillus subtilis is controlled by the competence-specific transcription factor ComK. ComK activates transcription of itself and several other genes required for competence. The activity of ComK is controlled by other genes including mecA, clpC, and comS. We have used purified ComK, MecA, ClpC, and synthetic ComS to study their interactions and have demonstrated the following mechanism for ComK regulation. ClpC, in the presence of ATP, forms a ternary complex with MecA and ComK, which prevents ComK from binding to its specific DNA target. This complex dissociates when ComS is added, liberating active ComK. ClpC and MecA function as a molecular switch, in which MecA confers molecular recognition, connecting ClpC to ComK and to ComS.
Keywords
- ClpC, ComK, competence, heat shock, HSP100, MecA
ASJC Scopus subject areas
- Biochemistry, Genetics and Molecular Biology(all)
- Genetics
- Biochemistry, Genetics and Molecular Biology(all)
- Developmental Biology
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In: Genes and Development, Vol. 11, No. 1, 01.01.1997, p. 119-128.
Research output: Contribution to journal › Article › Research › peer review
}
TY - JOUR
T1 - Biochemical characterization of a molecular switch involving the heat shock protein ClpC, which controls the activity of ComK, the competence transcription factor of Bacillus subtilis
AU - Turgay, Kürşad
AU - Hamoen, Leendert W.
AU - Venema, Gerard
AU - Dubnau, David
N1 - Copyright: Copyright 2017 Elsevier B.V., All rights reserved.
PY - 1997/1/1
Y1 - 1997/1/1
N2 - Development of genetic competence in Bacillus subtilis is controlled by the competence-specific transcription factor ComK. ComK activates transcription of itself and several other genes required for competence. The activity of ComK is controlled by other genes including mecA, clpC, and comS. We have used purified ComK, MecA, ClpC, and synthetic ComS to study their interactions and have demonstrated the following mechanism for ComK regulation. ClpC, in the presence of ATP, forms a ternary complex with MecA and ComK, which prevents ComK from binding to its specific DNA target. This complex dissociates when ComS is added, liberating active ComK. ClpC and MecA function as a molecular switch, in which MecA confers molecular recognition, connecting ClpC to ComK and to ComS.
AB - Development of genetic competence in Bacillus subtilis is controlled by the competence-specific transcription factor ComK. ComK activates transcription of itself and several other genes required for competence. The activity of ComK is controlled by other genes including mecA, clpC, and comS. We have used purified ComK, MecA, ClpC, and synthetic ComS to study their interactions and have demonstrated the following mechanism for ComK regulation. ClpC, in the presence of ATP, forms a ternary complex with MecA and ComK, which prevents ComK from binding to its specific DNA target. This complex dissociates when ComS is added, liberating active ComK. ClpC and MecA function as a molecular switch, in which MecA confers molecular recognition, connecting ClpC to ComK and to ComS.
KW - ClpC
KW - ComK
KW - competence
KW - heat shock
KW - HSP100
KW - MecA
UR - http://www.scopus.com/inward/record.url?scp=0031030242&partnerID=8YFLogxK
U2 - 10.1101/gad.11.1.119
DO - 10.1101/gad.11.1.119
M3 - Article
C2 - 9000055
AN - SCOPUS:0031030242
VL - 11
SP - 119
EP - 128
JO - Genes and Development
JF - Genes and Development
SN - 0890-9369
IS - 1
ER -