Details
| Original language | English |
|---|---|
| Article number | 1955 |
| Journal | Foods |
| Volume | 11 |
| Issue number | 13 |
| Publication status | Published - 1 Jul 2022 |
Abstract
In this study, the in vitro bioavailability of rosehip infusion phenolics, mainly catechin, as a response to conventional and non-thermal treatments by combining gastrointestinal digestion and a Caco-2 cell culture model, was investigated. After application of thermal treatment (TT, 85 ◦C/10 min), high pressure (HPP, 600 MPa/5 min) or pulsed electric field (PEF, 15 kJ/kg) processing, all samples were subjected to simulated gastrointestinal digestion. Then, the amount of maximum non-toxic digest ratio was determined by the cytotoxicity sulforhodamine B (SRB) assay. Next, Caco-2 cells were exposed to 1:5 (v/v) times diluted digests in order to simulate the transepithelial transportation of catechin. Results showed that non-thermally processed samples (5.19 and 4.62% for HPP and PEF, respectively) exhibited greater transportation across the epithelial cell layer compared to than that of the TT-treated sample (3.42%). The present study highlighted that HPP and PEF, as non-thermal treatments at optimized conditions for infusions or beverages, can be utilized in order to enhance the nutritional quality of the final products.
Keywords
- Caco-2 cell culture, LC-MS, bioactive compounds, metabolic fate, non-thermal processing, transepithelial transport
ASJC Scopus subject areas
- Agricultural and Biological Sciences(all)
- Food Science
- Immunology and Microbiology(all)
- Microbiology
- Social Sciences(all)
- Health(social science)
- Health Professions(all)
- Health Professions (miscellaneous)
- Agricultural and Biological Sciences(all)
- Plant Science
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In: Foods, Vol. 11, No. 13, 1955, 01.07.2022.
Research output: Contribution to journal › Article › Research › peer review
}
TY - JOUR
T1 - Bioavailability of rosehip (Rosa canina L.) infusion phenolics prepared by thermal, pulsed electric field and high pressure processing
AU - Ozkan, Gulay
AU - Esatbeyoglu, Tuba
AU - Capanoglu, Esra
N1 - Funding Information: The publication of this article was funded by the Open Access Fund of Leibniz Universität Hannover. The authors would like to acknowledge Gerald Dräger for his guidance during LC-MS analysis and Kemal Aganovic from the German Institute of Food Technologies DIL for providing the HPP and PEF devices.
PY - 2022/7/1
Y1 - 2022/7/1
N2 - In this study, the in vitro bioavailability of rosehip infusion phenolics, mainly catechin, as a response to conventional and non-thermal treatments by combining gastrointestinal digestion and a Caco-2 cell culture model, was investigated. After application of thermal treatment (TT, 85 ◦C/10 min), high pressure (HPP, 600 MPa/5 min) or pulsed electric field (PEF, 15 kJ/kg) processing, all samples were subjected to simulated gastrointestinal digestion. Then, the amount of maximum non-toxic digest ratio was determined by the cytotoxicity sulforhodamine B (SRB) assay. Next, Caco-2 cells were exposed to 1:5 (v/v) times diluted digests in order to simulate the transepithelial transportation of catechin. Results showed that non-thermally processed samples (5.19 and 4.62% for HPP and PEF, respectively) exhibited greater transportation across the epithelial cell layer compared to than that of the TT-treated sample (3.42%). The present study highlighted that HPP and PEF, as non-thermal treatments at optimized conditions for infusions or beverages, can be utilized in order to enhance the nutritional quality of the final products.
AB - In this study, the in vitro bioavailability of rosehip infusion phenolics, mainly catechin, as a response to conventional and non-thermal treatments by combining gastrointestinal digestion and a Caco-2 cell culture model, was investigated. After application of thermal treatment (TT, 85 ◦C/10 min), high pressure (HPP, 600 MPa/5 min) or pulsed electric field (PEF, 15 kJ/kg) processing, all samples were subjected to simulated gastrointestinal digestion. Then, the amount of maximum non-toxic digest ratio was determined by the cytotoxicity sulforhodamine B (SRB) assay. Next, Caco-2 cells were exposed to 1:5 (v/v) times diluted digests in order to simulate the transepithelial transportation of catechin. Results showed that non-thermally processed samples (5.19 and 4.62% for HPP and PEF, respectively) exhibited greater transportation across the epithelial cell layer compared to than that of the TT-treated sample (3.42%). The present study highlighted that HPP and PEF, as non-thermal treatments at optimized conditions for infusions or beverages, can be utilized in order to enhance the nutritional quality of the final products.
KW - Caco-2 cell culture
KW - LC-MS
KW - bioactive compounds
KW - metabolic fate
KW - non-thermal processing
KW - transepithelial transport
UR - http://www.scopus.com/inward/record.url?scp=85133501131&partnerID=8YFLogxK
U2 - 10.3390/foods11131955
DO - 10.3390/foods11131955
M3 - Article
VL - 11
JO - Foods
JF - Foods
IS - 13
M1 - 1955
ER -