Details
| Original language | English |
|---|---|
| Article number | 103439 |
| Journal | Additive Manufacturing |
| Volume | 64 |
| Early online date | 1 Feb 2023 |
| Publication status | Published - 25 Feb 2023 |
Abstract
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In: Additive Manufacturing, Vol. 64, 103439, 25.02.2023.
Research output: Contribution to journal › Article › Research › peer review
}
TY - JOUR
T1 - Bioactive photocrosslinkable resin solely based on refined decellularized small intestinal submucosa for digital light processing 3D printing of in vitro tissue mimics
AU - Elomaa, Laura
AU - Gerbeth, Lorenz
AU - Almalla, Ahed
AU - Fribiczer, Nora
AU - Tang, Peter
AU - Hillebrandt, Karl
AU - Sauer, Igor Maximillian
AU - Seiffert, Sebastian
AU - Siegmund, Britta
AU - Weinhart, Marie
N1 - Funding Information: The authors warmly thank Dahlem Research School and the Focus Area Nanoscale at Freie Universität Berlin (LE), the Federal Ministry of Education and Research (FKZ: 13N13523) (MW), and the German Research Foundation (DFG) for financial support of the current work within the Collaborative Research Centre CRC 1449 (431232613; MW, LE, LH, AA, BS) and the Research Unit FOR 2811 (NF, SS) (Project ID 431232613 and 423791428). Acknowledgment: M.Sc. Peng Tang is kindly acknowledged for taking the SEM images at the Core
PY - 2023/2/25
Y1 - 2023/2/25
N2 - Three-dimensionally (3D) printed tissue mimics are unique in vitro platforms for studying human pathophysiology in a more physiologically relevant manner compared to oversimplified 2D cell cultures and complex animal models. However, their 3D printing requires an availability of materials that at the same time show a high level of biomimicry and also have a suitable viscosity profile and crosslinking kinetics for the desired printing technique. We developed a new biomimetic material for vat photopolymerization by solubilizing and functionalizing porcine small intestine submucosa (dSIS) into photocrosslinkable dSIS methacryloyl (dSIS-MA) and by subsequently formulating it into a bioactive 3D printing resin. The concentration of 1.5 wt% of dSIS-MA yielded desired viscosity and photocrosslinking kinetics, and the 3D printing of the resin resulted in fully transparent and highly swelling dSIS-MA hydrogels with a stiffness resembling native intestinal tissue. The new dSIS-MA resin was successfully 3D printed into acellular intestine-mimicking scaffolds that desirably guided the seeded human intestinal cells to grow along the 3D villi mimics. Human small intestinal organoid-derived undifferentiated primary cells grew to confluency on the dSIS-MA hydrogels and formed continuous tight junctions, thereby demonstrating the suitability of the 3D printing material for growing intestinal epithelium mimics. Furthermore, a small fraction of the human primary intestinal cells produced mucin 5AC, demonstrating early differentiation of these cells on the dSIS-MA hydrogels. The excellent cell compatibility of the dSIS-MA material combined with its high printability and biomimicry indicated that this new resin can be a great help in modelling and reproducing native tissue architectures where enhanced physiological relevancy is desired.
AB - Three-dimensionally (3D) printed tissue mimics are unique in vitro platforms for studying human pathophysiology in a more physiologically relevant manner compared to oversimplified 2D cell cultures and complex animal models. However, their 3D printing requires an availability of materials that at the same time show a high level of biomimicry and also have a suitable viscosity profile and crosslinking kinetics for the desired printing technique. We developed a new biomimetic material for vat photopolymerization by solubilizing and functionalizing porcine small intestine submucosa (dSIS) into photocrosslinkable dSIS methacryloyl (dSIS-MA) and by subsequently formulating it into a bioactive 3D printing resin. The concentration of 1.5 wt% of dSIS-MA yielded desired viscosity and photocrosslinking kinetics, and the 3D printing of the resin resulted in fully transparent and highly swelling dSIS-MA hydrogels with a stiffness resembling native intestinal tissue. The new dSIS-MA resin was successfully 3D printed into acellular intestine-mimicking scaffolds that desirably guided the seeded human intestinal cells to grow along the 3D villi mimics. Human small intestinal organoid-derived undifferentiated primary cells grew to confluency on the dSIS-MA hydrogels and formed continuous tight junctions, thereby demonstrating the suitability of the 3D printing material for growing intestinal epithelium mimics. Furthermore, a small fraction of the human primary intestinal cells produced mucin 5AC, demonstrating early differentiation of these cells on the dSIS-MA hydrogels. The excellent cell compatibility of the dSIS-MA material combined with its high printability and biomimicry indicated that this new resin can be a great help in modelling and reproducing native tissue architectures where enhanced physiological relevancy is desired.
UR - https://www.scopus.com/record/display.uri?eid=2-s2.0-85149793021&origin=inward&txGid=f647da1c14d745c4a983fc35b00dfb00
U2 - 10.26434/chemrxiv-2022-f2hpc
DO - 10.26434/chemrxiv-2022-f2hpc
M3 - Article
VL - 64
JO - Additive Manufacturing
JF - Additive Manufacturing
M1 - 103439
ER -