Details
| Original language | English |
|---|---|
| Pages (from-to) | 555-562 |
| Number of pages | 8 |
| Journal | Journal of pest science |
| Volume | 88 |
| Issue number | 3 |
| Publication status | Published - 20 Sept 2015 |
Abstract
Endophytic fungi colonize healthy plant tissues and can in some cases induce systemic resistance to the host against biotic and abiotic stresses. In our previous study, Hypocrea lixii isolate F3ST1 was able to colonize onion plants endophytically and conferred resistance to them against onion thrips, Thrips tabaci. To further elucidate the mechanism of resistance, we examined the behavioral response of adult and larval stages of T. tabaci to endophyte-inoculated (E+) and endophyte-free (E−) onion plants/sections. In choice experiments, female T. tabaci preferred E− over E+ plants. The number of feeding punctures and eggs was more on E− than on E+ plants. Oviposition was reduced sixfold on E+ plants within a 72-h experimental period. In the Y-tube olfactometer assay, thrips showed a 3.3-fold preference for E− plants. In individual larval choice experiments, significantly more first and second instars were found on the leaf sections of E− as compared to the E+ plants. In the settlement preference assay with groups of second instars, more larvae preferred leaf sections from E− over E+ plants with incremental time. Our findings suggest that endophyte-colonized onion plants may trigger antixenotic repellence of T. tabaci, impacting their biology. This repellence could be exploited in thrips control programs by using endophyte-inoculated plants in the field.
Keywords
- Choice test, Endophytic fungus, Hypocrea lixii, Induced systemic resistance, Thrips
ASJC Scopus subject areas
- Agricultural and Biological Sciences(all)
- Agronomy and Crop Science
Cite this
- Standard
- Harvard
- Apa
- Vancouver
- BibTeX
- RIS
In: Journal of pest science, Vol. 88, No. 3, 20.09.2015, p. 555-562.
Research output: Contribution to journal › Article › Research › peer review
}
TY - JOUR
T1 - Behavioral responses of Thrips tabaci Lindeman to endophyte-inoculated onion plants
AU - Muvea, A. M.
AU - Meyhöfer, R.
AU - Maniania, N. K.
AU - Poehling, H. M.
AU - Ekesi, S.
AU - Subramanian, S.
N1 - Funding information: This study was funded by BMZ (The German Federal Ministry for Economic Cooperation and Development) through GIZ (Deutsche Gesellschaft für Internationale Zusammenarbeit) through a project grant entitled “Implementation of integrated thrips and tospovirus management strategies in small-holder vegetable cropping systems of Eastern Africa” (project no. 11.7860.7-001.00, contract no. 81141840) for which we are grateful. The authors thank the icipe Thrips IPM Project staff for their technical assistance.
PY - 2015/9/20
Y1 - 2015/9/20
N2 - Endophytic fungi colonize healthy plant tissues and can in some cases induce systemic resistance to the host against biotic and abiotic stresses. In our previous study, Hypocrea lixii isolate F3ST1 was able to colonize onion plants endophytically and conferred resistance to them against onion thrips, Thrips tabaci. To further elucidate the mechanism of resistance, we examined the behavioral response of adult and larval stages of T. tabaci to endophyte-inoculated (E+) and endophyte-free (E−) onion plants/sections. In choice experiments, female T. tabaci preferred E− over E+ plants. The number of feeding punctures and eggs was more on E− than on E+ plants. Oviposition was reduced sixfold on E+ plants within a 72-h experimental period. In the Y-tube olfactometer assay, thrips showed a 3.3-fold preference for E− plants. In individual larval choice experiments, significantly more first and second instars were found on the leaf sections of E− as compared to the E+ plants. In the settlement preference assay with groups of second instars, more larvae preferred leaf sections from E− over E+ plants with incremental time. Our findings suggest that endophyte-colonized onion plants may trigger antixenotic repellence of T. tabaci, impacting their biology. This repellence could be exploited in thrips control programs by using endophyte-inoculated plants in the field.
AB - Endophytic fungi colonize healthy plant tissues and can in some cases induce systemic resistance to the host against biotic and abiotic stresses. In our previous study, Hypocrea lixii isolate F3ST1 was able to colonize onion plants endophytically and conferred resistance to them against onion thrips, Thrips tabaci. To further elucidate the mechanism of resistance, we examined the behavioral response of adult and larval stages of T. tabaci to endophyte-inoculated (E+) and endophyte-free (E−) onion plants/sections. In choice experiments, female T. tabaci preferred E− over E+ plants. The number of feeding punctures and eggs was more on E− than on E+ plants. Oviposition was reduced sixfold on E+ plants within a 72-h experimental period. In the Y-tube olfactometer assay, thrips showed a 3.3-fold preference for E− plants. In individual larval choice experiments, significantly more first and second instars were found on the leaf sections of E− as compared to the E+ plants. In the settlement preference assay with groups of second instars, more larvae preferred leaf sections from E− over E+ plants with incremental time. Our findings suggest that endophyte-colonized onion plants may trigger antixenotic repellence of T. tabaci, impacting their biology. This repellence could be exploited in thrips control programs by using endophyte-inoculated plants in the field.
KW - Choice test
KW - Endophytic fungus
KW - Hypocrea lixii
KW - Induced systemic resistance
KW - Thrips
UR - http://www.scopus.com/inward/record.url?scp=84939466589&partnerID=8YFLogxK
U2 - 10.1007/s10340-015-0645-3
DO - 10.1007/s10340-015-0645-3
M3 - Article
AN - SCOPUS:84939466589
VL - 88
SP - 555
EP - 562
JO - Journal of pest science
JF - Journal of pest science
SN - 1612-4758
IS - 3
ER -