Beet mosaic virus expression of a betalain transcription factor allows visual virus tracking in Beta vulgaris

Research output: Contribution to journalArticleResearchpeer review

Authors

  • Lukas Rollwage
  • Edgar Maiss
  • Wulf Menzel
  • Roxana Hossain
  • Mark Varrelmann

External Research Organisations

  • Institut für Zuckerrübenforschung (IfZ)
  • Leibniz Institute DSMZ-German Collection of Microorganisms and Cell Cultures
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Details

Original languageEnglish
Pages (from-to)1319-1329
Number of pages11
JournalMolecular plant pathology
Volume24
Issue number10
Publication statusPublished - 15 Sept 2023

Abstract

In the field of plant virology, the usage of reverse genetic systems has been reported for multiple purposes. One is understanding virus–host interaction by labelling viral cDNA clones with fluorescent protein genes to allow visual virus tracking throughout a plant, albeit this visualization depends on technical devices. Here we report the first construction of an infectious cDNA full-length clone of beet mosaic virus (BtMV) that can be efficiently used for Agrobacterium-mediated leaf inoculation with high infection rate in Beta vulgaris, being indistinguishable from the natural virus isolate regarding symptom development and vector transmission. Furthermore, the BtMV clone was tagged with the genes for the monomeric red fluorescent protein or the Beta vulgaris BvMYB1 transcription factor, which activates the betalain biosynthesis pathway. The heterologous expression of BvMYB1 results in activation of betalain biosynthesis genes in planta, allowing visualization of the systemic BtMV spread with the naked eye as red pigmentation emerging throughout beet leaves. In the case of BtMV, the BvMYB1 marker system is stable over multiple mechanical host passages, allows qualitative as well as quantitative virus detection and offers an excellent opportunity to label viruses in plants of the order Caryophyllales, allowing an in-depth investigation of virus–host interactions on the whole plant level.

Keywords

    beet mosaic virus, betalain biosynthesis, BvMYB1, Caryophyllales, infectious cDNA clone, virus tracking

ASJC Scopus subject areas

Cite this

Beet mosaic virus expression of a betalain transcription factor allows visual virus tracking in Beta vulgaris. / Rollwage, Lukas; Maiss, Edgar; Menzel, Wulf et al.
In: Molecular plant pathology, Vol. 24, No. 10, 15.09.2023, p. 1319-1329.

Research output: Contribution to journalArticleResearchpeer review

Rollwage L, Maiss E, Menzel W, Hossain R, Varrelmann M. Beet mosaic virus expression of a betalain transcription factor allows visual virus tracking in Beta vulgaris. Molecular plant pathology. 2023 Sept 15;24(10):1319-1329. doi: 10.1111/mpp.13372
Rollwage, Lukas ; Maiss, Edgar ; Menzel, Wulf et al. / Beet mosaic virus expression of a betalain transcription factor allows visual virus tracking in Beta vulgaris. In: Molecular plant pathology. 2023 ; Vol. 24, No. 10. pp. 1319-1329.
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abstract = "In the field of plant virology, the usage of reverse genetic systems has been reported for multiple purposes. One is understanding virus–host interaction by labelling viral cDNA clones with fluorescent protein genes to allow visual virus tracking throughout a plant, albeit this visualization depends on technical devices. Here we report the first construction of an infectious cDNA full-length clone of beet mosaic virus (BtMV) that can be efficiently used for Agrobacterium-mediated leaf inoculation with high infection rate in Beta vulgaris, being indistinguishable from the natural virus isolate regarding symptom development and vector transmission. Furthermore, the BtMV clone was tagged with the genes for the monomeric red fluorescent protein or the Beta vulgaris BvMYB1 transcription factor, which activates the betalain biosynthesis pathway. The heterologous expression of BvMYB1 results in activation of betalain biosynthesis genes in planta, allowing visualization of the systemic BtMV spread with the naked eye as red pigmentation emerging throughout beet leaves. In the case of BtMV, the BvMYB1 marker system is stable over multiple mechanical host passages, allows qualitative as well as quantitative virus detection and offers an excellent opportunity to label viruses in plants of the order Caryophyllales, allowing an in-depth investigation of virus–host interactions on the whole plant level.",
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