Loading [MathJax]/extensions/tex2jax.js

Bacterial glycolipids and analogs as antigens for CD1d-restricted NKT cells

Research output: Contribution to journalArticleResearchpeer review

Authors

  • Douglass Wu
  • Guo Wen Xing
  • Michael A. Poles
  • Amir Horowitz
  • Oliver Plettenburg

External Research Organisations

  • The Scripps Research Translational Institute
  • Rockefeller University
  • New York University (NYU)
  • La Jolla Institute for Allergy and Immunology
Plum Print visual indicator of research metrics
  • Citations
    • Citation Indexes: 215
    • Patent Family Citations: 1
  • Captures
    • Readers: 98
see details

Details

Original languageEnglish
Pages (from-to)1351-1356
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume102
Issue number5
Early online date21 Jan 2005
Publication statusPublished - 1 Feb 2005
Externally publishedYes

Abstract

The CD1 family of proteins binds self and foreign glycolipids for presentation to CD1-restricted T cells. To identify previously uncharacterized active CD1 ligands, especially those of microbial origin, numerous glycolipids were synthesized and tested for their ability to stimulate mouse and human natural killer T (NKT) cells. They included analogs of the well known NKT cell agonist α-galactosyl ceramide (α-GalCer), bacterial glycolipids, and variations of the self-glycolipid, sulfatide. Bacterial glycolipids, α-galacturonosyl-ceramides from Sphingomonas wittichii, although structurally similar to α-GalCer, have significant differences in the sugar head group as well as the ceramide portion. The Sphingomonas glycosphingolipids (GSLs) and sulfatide variants were shown to activate human NKT cells as measured by IL-4 and IFN-γ secretion. Moreover, CD1d-dimer staining revealed human NKT cell reactivity toward these GSLs and to the sulfatides in a fashion comparable with α-GalCer. Because α-GalCer is a marine-sponge-derived ligand, our study here shows that bacterium-derived antigens are also able to stimulate mouse and human NKT cells.

ASJC Scopus subject areas

Sustainable Development Goals

Cite this

Bacterial glycolipids and analogs as antigens for CD1d-restricted NKT cells. / Wu, Douglass; Xing, Guo Wen; Poles, Michael A. et al.
In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 102, No. 5, 01.02.2005, p. 1351-1356.

Research output: Contribution to journalArticleResearchpeer review

Wu, D, Xing, GW, Poles, MA, Horowitz, A, Kinjo, Y, Sullivan, B, Bodmer-Narkevitch, V, Plettenburg, O, Kronenberg, M, Tsuji, M, Ho, DD & Wong, CH 2005, 'Bacterial glycolipids and analogs as antigens for CD1d-restricted NKT cells', Proceedings of the National Academy of Sciences of the United States of America, vol. 102, no. 5, pp. 1351-1356. https://doi.org/10.1073/pnas.0408696102
Wu, D., Xing, G. W., Poles, M. A., Horowitz, A., Kinjo, Y., Sullivan, B., Bodmer-Narkevitch, V., Plettenburg, O., Kronenberg, M., Tsuji, M., Ho, D. D., & Wong, C. H. (2005). Bacterial glycolipids and analogs as antigens for CD1d-restricted NKT cells. Proceedings of the National Academy of Sciences of the United States of America, 102(5), 1351-1356. https://doi.org/10.1073/pnas.0408696102
Wu D, Xing GW, Poles MA, Horowitz A, Kinjo Y, Sullivan B et al. Bacterial glycolipids and analogs as antigens for CD1d-restricted NKT cells. Proceedings of the National Academy of Sciences of the United States of America. 2005 Feb 1;102(5):1351-1356. Epub 2005 Jan 21. doi: 10.1073/pnas.0408696102
Wu, Douglass ; Xing, Guo Wen ; Poles, Michael A. et al. / Bacterial glycolipids and analogs as antigens for CD1d-restricted NKT cells. In: Proceedings of the National Academy of Sciences of the United States of America. 2005 ; Vol. 102, No. 5. pp. 1351-1356.
Download
@article{0da76371df7647e0a05b2c77bcd4982b,
title = "Bacterial glycolipids and analogs as antigens for CD1d-restricted NKT cells",
abstract = "The CD1 family of proteins binds self and foreign glycolipids for presentation to CD1-restricted T cells. To identify previously uncharacterized active CD1 ligands, especially those of microbial origin, numerous glycolipids were synthesized and tested for their ability to stimulate mouse and human natural killer T (NKT) cells. They included analogs of the well known NKT cell agonist α-galactosyl ceramide (α-GalCer), bacterial glycolipids, and variations of the self-glycolipid, sulfatide. Bacterial glycolipids, α-galacturonosyl-ceramides from Sphingomonas wittichii, although structurally similar to α-GalCer, have significant differences in the sugar head group as well as the ceramide portion. The Sphingomonas glycosphingolipids (GSLs) and sulfatide variants were shown to activate human NKT cells as measured by IL-4 and IFN-γ secretion. Moreover, CD1d-dimer staining revealed human NKT cell reactivity toward these GSLs and to the sulfatides in a fashion comparable with α-GalCer. Because α-GalCer is a marine-sponge-derived ligand, our study here shows that bacterium-derived antigens are also able to stimulate mouse and human NKT cells.",
author = "Douglass Wu and Xing, {Guo Wen} and Poles, {Michael A.} and Amir Horowitz and Yuki Kinjo and Barbara Sullivan and Vera Bodmer-Narkevitch and Oliver Plettenburg and Mitchell Kronenberg and Moriya Tsuji and Ho, {David D.} and Wong, {Chi Huey}",
year = "2005",
month = feb,
day = "1",
doi = "10.1073/pnas.0408696102",
language = "English",
volume = "102",
pages = "1351--1356",
journal = "Proceedings of the National Academy of Sciences of the United States of America",
issn = "0027-8424",
publisher = "National Academy of Sciences",
number = "5",

}

Download

TY - JOUR

T1 - Bacterial glycolipids and analogs as antigens for CD1d-restricted NKT cells

AU - Wu, Douglass

AU - Xing, Guo Wen

AU - Poles, Michael A.

AU - Horowitz, Amir

AU - Kinjo, Yuki

AU - Sullivan, Barbara

AU - Bodmer-Narkevitch, Vera

AU - Plettenburg, Oliver

AU - Kronenberg, Mitchell

AU - Tsuji, Moriya

AU - Ho, David D.

AU - Wong, Chi Huey

PY - 2005/2/1

Y1 - 2005/2/1

N2 - The CD1 family of proteins binds self and foreign glycolipids for presentation to CD1-restricted T cells. To identify previously uncharacterized active CD1 ligands, especially those of microbial origin, numerous glycolipids were synthesized and tested for their ability to stimulate mouse and human natural killer T (NKT) cells. They included analogs of the well known NKT cell agonist α-galactosyl ceramide (α-GalCer), bacterial glycolipids, and variations of the self-glycolipid, sulfatide. Bacterial glycolipids, α-galacturonosyl-ceramides from Sphingomonas wittichii, although structurally similar to α-GalCer, have significant differences in the sugar head group as well as the ceramide portion. The Sphingomonas glycosphingolipids (GSLs) and sulfatide variants were shown to activate human NKT cells as measured by IL-4 and IFN-γ secretion. Moreover, CD1d-dimer staining revealed human NKT cell reactivity toward these GSLs and to the sulfatides in a fashion comparable with α-GalCer. Because α-GalCer is a marine-sponge-derived ligand, our study here shows that bacterium-derived antigens are also able to stimulate mouse and human NKT cells.

AB - The CD1 family of proteins binds self and foreign glycolipids for presentation to CD1-restricted T cells. To identify previously uncharacterized active CD1 ligands, especially those of microbial origin, numerous glycolipids were synthesized and tested for their ability to stimulate mouse and human natural killer T (NKT) cells. They included analogs of the well known NKT cell agonist α-galactosyl ceramide (α-GalCer), bacterial glycolipids, and variations of the self-glycolipid, sulfatide. Bacterial glycolipids, α-galacturonosyl-ceramides from Sphingomonas wittichii, although structurally similar to α-GalCer, have significant differences in the sugar head group as well as the ceramide portion. The Sphingomonas glycosphingolipids (GSLs) and sulfatide variants were shown to activate human NKT cells as measured by IL-4 and IFN-γ secretion. Moreover, CD1d-dimer staining revealed human NKT cell reactivity toward these GSLs and to the sulfatides in a fashion comparable with α-GalCer. Because α-GalCer is a marine-sponge-derived ligand, our study here shows that bacterium-derived antigens are also able to stimulate mouse and human NKT cells.

UR - http://www.scopus.com/inward/record.url?scp=13444255993&partnerID=8YFLogxK

U2 - 10.1073/pnas.0408696102

DO - 10.1073/pnas.0408696102

M3 - Article

C2 - 15665086

AN - SCOPUS:13444255993

VL - 102

SP - 1351

EP - 1356

JO - Proceedings of the National Academy of Sciences of the United States of America

JF - Proceedings of the National Academy of Sciences of the United States of America

SN - 0027-8424

IS - 5

ER -

By the same author(s)