Details
| Original language | English |
|---|---|
| Article number | 139583 |
| Journal | THIN SOLID FILMS |
| Volume | 764 |
| Early online date | 13 Nov 2022 |
| Publication status | Published - 1 Jan 2023 |
Abstract
In this work, we investigate whether bacteria can be substantially examined in liquid environment with an ellipsometer in the visible wavelength range. For that investigation a Kretschmann type flow cell was used in which the paths of illuminated and reflected light beams are on the substrate side. To enhance the sensitivity a thin gold layer was applied on the top of glass substrate and the measurement was performed by using surface plasmon polaritons. Porphyrin functionalized coating layers were employed to analyze their performance to capture bacteria and to study the stability and repeatability of the optical response with regard to the ellipsometric measurement. Escherichia coli (E. coli) bacteria were used as first model bacteria and their deposition on the sensing surface was monitored in a real-time measurement in a liquid environment.
Keywords
- Bacteria detection, Bio-ellipsometry, Escherichia coli, Kretschmann flow cell, Surface plasmon
ASJC Scopus subject areas
- Materials Science(all)
- Electronic, Optical and Magnetic Materials
- Physics and Astronomy(all)
- Surfaces and Interfaces
- Materials Science(all)
- Surfaces, Coatings and Films
- Materials Science(all)
- Metals and Alloys
- Materials Science(all)
- Materials Chemistry
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In: THIN SOLID FILMS, Vol. 764, 139583, 01.01.2023.
Research output: Contribution to journal › Article › Research › peer review
}
TY - JOUR
T1 - Bacteria detection in a Kretschmann geometry flow cell at a plasmon-enhanced interface with spectroscopic ellipsometer
AU - Zangenehzadeh, Soraya
AU - Agócs, Emil
AU - Jivani, Harsh
AU - Könemund, Lea
AU - Neumann, Laurie
AU - Hirschberg, Felix
AU - Herdan, Svenja
AU - Biedendieck, Rebekka
AU - Jahn, Dieter
AU - Roth, Bernhard W.
AU - Johannes, Hans Hermann
AU - Kowalsky, Wolfgang
N1 - Funding Information: We would like to thank Gabriele Günther (BRICS, TU BS, Braunschweig, Germany), Kathleen Möhring, and Nassima Amroun (IHF, TU BS, Braunschweig, Germany) for technical assistance. We gratefully acknowledge Germany’s Excellence Strategy within the Cluster of Excellence PhoenixD (EXC 2122, Project ID 390833453 ) for financial support and the Volkswagen Foundation, Germany for funding Project Laboratory Across Borders (ProLAB) project number 96620.
PY - 2023/1/1
Y1 - 2023/1/1
N2 - In this work, we investigate whether bacteria can be substantially examined in liquid environment with an ellipsometer in the visible wavelength range. For that investigation a Kretschmann type flow cell was used in which the paths of illuminated and reflected light beams are on the substrate side. To enhance the sensitivity a thin gold layer was applied on the top of glass substrate and the measurement was performed by using surface plasmon polaritons. Porphyrin functionalized coating layers were employed to analyze their performance to capture bacteria and to study the stability and repeatability of the optical response with regard to the ellipsometric measurement. Escherichia coli (E. coli) bacteria were used as first model bacteria and their deposition on the sensing surface was monitored in a real-time measurement in a liquid environment.
AB - In this work, we investigate whether bacteria can be substantially examined in liquid environment with an ellipsometer in the visible wavelength range. For that investigation a Kretschmann type flow cell was used in which the paths of illuminated and reflected light beams are on the substrate side. To enhance the sensitivity a thin gold layer was applied on the top of glass substrate and the measurement was performed by using surface plasmon polaritons. Porphyrin functionalized coating layers were employed to analyze their performance to capture bacteria and to study the stability and repeatability of the optical response with regard to the ellipsometric measurement. Escherichia coli (E. coli) bacteria were used as first model bacteria and their deposition on the sensing surface was monitored in a real-time measurement in a liquid environment.
KW - Bacteria detection
KW - Bio-ellipsometry
KW - Escherichia coli
KW - Kretschmann flow cell
KW - Surface plasmon
UR - http://www.scopus.com/inward/record.url?scp=85145592216&partnerID=8YFLogxK
U2 - 10.1016/j.tsf.2022.139583
DO - 10.1016/j.tsf.2022.139583
M3 - Article
AN - SCOPUS:85145592216
VL - 764
JO - THIN SOLID FILMS
JF - THIN SOLID FILMS
SN - 0040-6090
M1 - 139583
ER -