Automatic tracking of swimming microorganisms in 4D digital in-line holography data

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  • Heidelberg University
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Original languageEnglish
Title of host publication2009 Workshop on Motion and Video Computing, WMVC '09
Publication statusPublished - 2009
Event2009 Workshop on Motion and Video Computing, WMVC '09 - Snowbird, UT, United States
Duration: 8 Dec 20099 Dec 2009

Publication series

Name2009 Workshop on Motion and Video Computing, WMVC '09

Abstract

Digital in-line holography is a microscopy technique which has gotten an increasing amount of attention over the last few years in the fields of microbiology, medicine and physics, as it provides an efficient way of measuring 3D microscopic data over time. In this paper we approach the challenges of a high throughput analysis of holographic microscopy data and present a system for detecting particles in 3D reconstructed holograms and their 3D trajectory estimation over time. Our main contribution is a robust method which evolves from the Hungarian bipartite weighted graph matching algorithm and allows us to deal with newly entering and leaving particles and compensate for missing data and outliers. In the experiments we compare our fully automatic system with manually labeled ground truth data and we can report an accuracy between 76% and 91%.

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Automatic tracking of swimming microorganisms in 4D digital in-line holography data. / Leal Taixé, Laura; Heydt, Matthias; Rosenhahn, Axel et al.
2009 Workshop on Motion and Video Computing, WMVC '09. 2009. 5399244 (2009 Workshop on Motion and Video Computing, WMVC '09).

Research output: Chapter in book/report/conference proceedingConference contributionResearchpeer review

Leal Taixé, L, Heydt, M, Rosenhahn, A & Rosenhahn, B 2009, Automatic tracking of swimming microorganisms in 4D digital in-line holography data. in 2009 Workshop on Motion and Video Computing, WMVC '09., 5399244, 2009 Workshop on Motion and Video Computing, WMVC '09, 2009 Workshop on Motion and Video Computing, WMVC '09, Snowbird, UT, United States, 8 Dec 2009. https://doi.org/10.1109/WMVC.2009.5399244
Leal Taixé, L., Heydt, M., Rosenhahn, A., & Rosenhahn, B. (2009). Automatic tracking of swimming microorganisms in 4D digital in-line holography data. In 2009 Workshop on Motion and Video Computing, WMVC '09 Article 5399244 (2009 Workshop on Motion and Video Computing, WMVC '09). https://doi.org/10.1109/WMVC.2009.5399244
Leal Taixé L, Heydt M, Rosenhahn A, Rosenhahn B. Automatic tracking of swimming microorganisms in 4D digital in-line holography data. In 2009 Workshop on Motion and Video Computing, WMVC '09. 2009. 5399244. (2009 Workshop on Motion and Video Computing, WMVC '09). doi: 10.1109/WMVC.2009.5399244
Leal Taixé, Laura ; Heydt, Matthias ; Rosenhahn, Axel et al. / Automatic tracking of swimming microorganisms in 4D digital in-line holography data. 2009 Workshop on Motion and Video Computing, WMVC '09. 2009. (2009 Workshop on Motion and Video Computing, WMVC '09).
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