Details
| Original language | English |
|---|---|
| Pages (from-to) | 463-468 |
| Number of pages | 6 |
| Journal | Chemical Engineering and Technology |
| Volume | 31 |
| Issue number | 3 |
| Publication status | Published - 28 Feb 2008 |
Abstract
To achieve standardized and parallelized microscale protein purification, an automated purification system was developed and optimized using a pipetting robot for purifying large sets of proteins with Ni-NTA magnetic agarose beads. Recombinant hexahistidine-tagged proteins β-glucanase (Bgl-His) and esterase I of Pseudomonas fluorescens (PFE I) were tested on the system. High purity along with high coherence was achieved. The purification procedure can be applied to 96 samples simultaneously and only takes about 1/3 of the time required for purification conducted by hand. Due to the stability of the robot system, the consistency of the results is much better than the results achieved through manual purification. This rapid and reliable method is reproducible and applicable to high throughput screening of huge amounts of samples.
Keywords
- Magnetic agarose beads, Optimization, Protein purification
ASJC Scopus subject areas
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In: Chemical Engineering and Technology, Vol. 31, No. 3, 28.02.2008, p. 463-468.
Research output: Contribution to journal › Article › Research › peer review
}
TY - JOUR
T1 - Automated Microscale His-tagged ProteinPurification Using Ni-NTA Magnetic Agarose Beads
AU - Meng, Jin
AU - Walter, Johanna Gabriela
AU - Kökpinar, Öznur
AU - Stahl, Frank
AU - Scheper, Thomas
PY - 2008/2/28
Y1 - 2008/2/28
N2 - To achieve standardized and parallelized microscale protein purification, an automated purification system was developed and optimized using a pipetting robot for purifying large sets of proteins with Ni-NTA magnetic agarose beads. Recombinant hexahistidine-tagged proteins β-glucanase (Bgl-His) and esterase I of Pseudomonas fluorescens (PFE I) were tested on the system. High purity along with high coherence was achieved. The purification procedure can be applied to 96 samples simultaneously and only takes about 1/3 of the time required for purification conducted by hand. Due to the stability of the robot system, the consistency of the results is much better than the results achieved through manual purification. This rapid and reliable method is reproducible and applicable to high throughput screening of huge amounts of samples.
AB - To achieve standardized and parallelized microscale protein purification, an automated purification system was developed and optimized using a pipetting robot for purifying large sets of proteins with Ni-NTA magnetic agarose beads. Recombinant hexahistidine-tagged proteins β-glucanase (Bgl-His) and esterase I of Pseudomonas fluorescens (PFE I) were tested on the system. High purity along with high coherence was achieved. The purification procedure can be applied to 96 samples simultaneously and only takes about 1/3 of the time required for purification conducted by hand. Due to the stability of the robot system, the consistency of the results is much better than the results achieved through manual purification. This rapid and reliable method is reproducible and applicable to high throughput screening of huge amounts of samples.
KW - Magnetic agarose beads
KW - Optimization
KW - Protein purification
UR - http://www.scopus.com/inward/record.url?scp=41149086886&partnerID=8YFLogxK
U2 - 10.1002/ceat.200700429
DO - 10.1002/ceat.200700429
M3 - Article
AN - SCOPUS:41149086886
VL - 31
SP - 463
EP - 468
JO - Chemical Engineering and Technology
JF - Chemical Engineering and Technology
SN - 0930-7516
IS - 3
ER -