Details
| Original language | English |
|---|---|
| Pages (from-to) | 259-269 |
| Number of pages | 11 |
| Journal | CYTOTECHNOLOGY |
| Volume | 15 |
| Issue number | 1-3 |
| Publication status | Published - Feb 1994 |
| Externally published | Yes |
Abstract
Two different automated immunoanalysis systems are presented. Both are based on the principles of flow-injection analysis and were developed to provide reliable, rapid monitoring of relevant proteins in animal cell cultivation processes. One system uses a turbidimetric analysis, and the other employs a heterogeneous chemistry with immobilized immunocomponents. For both systems, the analysis time is in the range of a few minutes, and a complete analysis cycle, including triplicate analyses and various washing steps, is in the range of 20-30 minutes. Samples from cultivation processes can be analyzed directly without dilution. Quantitation of proteins such as rt-PA or monoclonal antibodies can be performed over an analyte concentration range of 1-1000 mg/L. Both systems were compared to conventional ELISA assays on microtiter plates. The turbidimetric analysis system also included a biosensor for simultaneous glucose determination.
Keywords
- Automated immunoanalysis, flow-injection analysis, heterogeneous assay, process analysis, turbidimetric assay
ASJC Scopus subject areas
- Biochemistry, Genetics and Molecular Biology(all)
- Biotechnology
- Chemical Engineering(all)
- Bioengineering
- Engineering(all)
- Biomedical Engineering
- Biochemistry, Genetics and Molecular Biology(all)
- Clinical Biochemistry
- Biochemistry, Genetics and Molecular Biology(all)
- Cell Biology
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In: CYTOTECHNOLOGY, Vol. 15, No. 1-3, 02.1994, p. 259-269.
Research output: Contribution to journal › Article › Research › peer review
}
TY - JOUR
T1 - Automated imunoanalysis systems for monitoring mammalian cell cultivation processes
AU - Schulze, Birgitt
AU - Middendorf, Cornelia
AU - Reinecke, Martin
AU - Scheper, Thomas
AU - Noé, Wolfgang
AU - Howaldt, Michael
PY - 1994/2
Y1 - 1994/2
N2 - Two different automated immunoanalysis systems are presented. Both are based on the principles of flow-injection analysis and were developed to provide reliable, rapid monitoring of relevant proteins in animal cell cultivation processes. One system uses a turbidimetric analysis, and the other employs a heterogeneous chemistry with immobilized immunocomponents. For both systems, the analysis time is in the range of a few minutes, and a complete analysis cycle, including triplicate analyses and various washing steps, is in the range of 20-30 minutes. Samples from cultivation processes can be analyzed directly without dilution. Quantitation of proteins such as rt-PA or monoclonal antibodies can be performed over an analyte concentration range of 1-1000 mg/L. Both systems were compared to conventional ELISA assays on microtiter plates. The turbidimetric analysis system also included a biosensor for simultaneous glucose determination.
AB - Two different automated immunoanalysis systems are presented. Both are based on the principles of flow-injection analysis and were developed to provide reliable, rapid monitoring of relevant proteins in animal cell cultivation processes. One system uses a turbidimetric analysis, and the other employs a heterogeneous chemistry with immobilized immunocomponents. For both systems, the analysis time is in the range of a few minutes, and a complete analysis cycle, including triplicate analyses and various washing steps, is in the range of 20-30 minutes. Samples from cultivation processes can be analyzed directly without dilution. Quantitation of proteins such as rt-PA or monoclonal antibodies can be performed over an analyte concentration range of 1-1000 mg/L. Both systems were compared to conventional ELISA assays on microtiter plates. The turbidimetric analysis system also included a biosensor for simultaneous glucose determination.
KW - Automated immunoanalysis
KW - flow-injection analysis
KW - heterogeneous assay
KW - process analysis
KW - turbidimetric assay
UR - http://www.scopus.com/inward/record.url?scp=0028693802&partnerID=8YFLogxK
U2 - 10.1007/BF00762401
DO - 10.1007/BF00762401
M3 - Article
C2 - 7765939
AN - SCOPUS:0028693802
VL - 15
SP - 259
EP - 269
JO - CYTOTECHNOLOGY
JF - CYTOTECHNOLOGY
SN - 0920-9069
IS - 1-3
ER -