Details
| Original language | English |
|---|---|
| Pages (from-to) | 443-446 |
| Number of pages | 4 |
| Journal | European Food Research and Technology |
| Volume | 215 |
| Issue number | 5 |
| Publication status | Published - 2002 |
| Externally published | Yes |
Abstract
There is an increasing need for high-throughput analyses of plants and food samples for the presence of specific DNA sequences, e.g. transgenic contaminations. We developed and optimized conditions for the automated isolation of DNA from several maize tissues and various edibles containing maize using the MagNA Pure LC system (Roche Applied Science). Our results show that the system provided is capable of isolating DNA from any tested source. Quantification of an endogenous gene by LightCycler real-time PCR revealed that the DNA is suitable in quality and quantity for multiple PCR analyses.
Keywords
- Automatization, DNA isolation, Food samples, Magnapure, Maize, Real-Time PCR
ASJC Scopus subject areas
- Biochemistry, Genetics and Molecular Biology(all)
- Biotechnology
- Agricultural and Biological Sciences(all)
- Food Science
- Biochemistry, Genetics and Molecular Biology(all)
- Biochemistry
- Chemistry(all)
- General Chemistry
- Engineering(all)
- Industrial and Manufacturing Engineering
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In: European Food Research and Technology, Vol. 215, No. 5, 2002, p. 443-446.
Research output: Contribution to journal › Article › Research › peer review
}
TY - JOUR
T1 - Automated DNA preparation from maize tissues and food samples suitable for real-time PCR detection of native genes
AU - Hahnen, Silke
AU - Offermann, Sascha
AU - Miedl, Brigitte
AU - Rüger, Barbara
AU - Peterhänsel, Christoph
PY - 2002
Y1 - 2002
N2 - There is an increasing need for high-throughput analyses of plants and food samples for the presence of specific DNA sequences, e.g. transgenic contaminations. We developed and optimized conditions for the automated isolation of DNA from several maize tissues and various edibles containing maize using the MagNA Pure LC system (Roche Applied Science). Our results show that the system provided is capable of isolating DNA from any tested source. Quantification of an endogenous gene by LightCycler real-time PCR revealed that the DNA is suitable in quality and quantity for multiple PCR analyses.
AB - There is an increasing need for high-throughput analyses of plants and food samples for the presence of specific DNA sequences, e.g. transgenic contaminations. We developed and optimized conditions for the automated isolation of DNA from several maize tissues and various edibles containing maize using the MagNA Pure LC system (Roche Applied Science). Our results show that the system provided is capable of isolating DNA from any tested source. Quantification of an endogenous gene by LightCycler real-time PCR revealed that the DNA is suitable in quality and quantity for multiple PCR analyses.
KW - Automatization
KW - DNA isolation
KW - Food samples
KW - Magnapure
KW - Maize
KW - Real-Time PCR
UR - http://www.scopus.com/inward/record.url?scp=1042299401&partnerID=8YFLogxK
U2 - 10.1007/s00217-002-0579-x
DO - 10.1007/s00217-002-0579-x
M3 - Article
AN - SCOPUS:1042299401
VL - 215
SP - 443
EP - 446
JO - European Food Research and Technology
JF - European Food Research and Technology
SN - 1438-2377
IS - 5
ER -