Details
| Original language | English |
|---|---|
| Pages (from-to) | 585-594 |
| Number of pages | 10 |
| Journal | CHEMBIOCHEM |
| Volume | 9 |
| Issue number | 4 |
| Publication status | Published - 3 Mar 2008 |
| Externally published | Yes |
Abstract
The tenS gene encoding tenellin synthetase (TENS), a 4239-residue polyketide synthase nonribosomal-peptide synthetase (PKSNRPS) from Beauveria bassiana, was expressed in Aspergillus oryzae M-2-3. This led to the production of three new compounds, identified as acyl tetramic acids, and numerous minor metabolites. Consideration of the structures of these compounds indicates that the putative C-terminal thiolester reductase (R) domain does not act as a reductase, but appears to act as a Dieckmann cyclase (DKC). Expression of tenS in the absence of a trans-acting ER component encoded by orf3 led to errors in assembly of the polyketide component, giving clues to the mode of programming of highly reducing fungal PKS. Coexpression of tenS with orf3 from the linked gene cluster led to the production of a correctly elaborated polyketide. The NRPS adenylation domain possibly shows the first identified fungal signature sequences for tyrosine selectivity.
Keywords
- Beauveria, Biosynthesis, Fungi, Heterologous expression, Natural products
ASJC Scopus subject areas
- Biochemistry, Genetics and Molecular Biology(all)
- Biochemistry
- Biochemistry, Genetics and Molecular Biology(all)
- Molecular Medicine
- Biochemistry, Genetics and Molecular Biology(all)
- Molecular Biology
- Chemistry(all)
- Organic Chemistry
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In: CHEMBIOCHEM, Vol. 9, No. 4, 03.03.2008, p. 585-594.
Research output: Contribution to journal › Article › Research › peer review
}
TY - JOUR
T1 - Authentic heterologous expression of the tenellin iterative polyketide synthase nonribosomal peptide synthetase requires cooexpression with an enoyl reductase
AU - Halo, Laura M.
AU - Marshall, James W.
AU - Yakasai, Ahmed A.
AU - Song, Zhongshu
AU - Butts, Craig P.
AU - Crump, Matthew P.
AU - Heneghan, Mary
AU - Bailey, Andrew M.
AU - Simpson, Thomas J.
AU - Lazarus, Colin M.
AU - Cox, Russell J.
PY - 2008/3/3
Y1 - 2008/3/3
N2 - The tenS gene encoding tenellin synthetase (TENS), a 4239-residue polyketide synthase nonribosomal-peptide synthetase (PKSNRPS) from Beauveria bassiana, was expressed in Aspergillus oryzae M-2-3. This led to the production of three new compounds, identified as acyl tetramic acids, and numerous minor metabolites. Consideration of the structures of these compounds indicates that the putative C-terminal thiolester reductase (R) domain does not act as a reductase, but appears to act as a Dieckmann cyclase (DKC). Expression of tenS in the absence of a trans-acting ER component encoded by orf3 led to errors in assembly of the polyketide component, giving clues to the mode of programming of highly reducing fungal PKS. Coexpression of tenS with orf3 from the linked gene cluster led to the production of a correctly elaborated polyketide. The NRPS adenylation domain possibly shows the first identified fungal signature sequences for tyrosine selectivity.
AB - The tenS gene encoding tenellin synthetase (TENS), a 4239-residue polyketide synthase nonribosomal-peptide synthetase (PKSNRPS) from Beauveria bassiana, was expressed in Aspergillus oryzae M-2-3. This led to the production of three new compounds, identified as acyl tetramic acids, and numerous minor metabolites. Consideration of the structures of these compounds indicates that the putative C-terminal thiolester reductase (R) domain does not act as a reductase, but appears to act as a Dieckmann cyclase (DKC). Expression of tenS in the absence of a trans-acting ER component encoded by orf3 led to errors in assembly of the polyketide component, giving clues to the mode of programming of highly reducing fungal PKS. Coexpression of tenS with orf3 from the linked gene cluster led to the production of a correctly elaborated polyketide. The NRPS adenylation domain possibly shows the first identified fungal signature sequences for tyrosine selectivity.
KW - Beauveria
KW - Biosynthesis
KW - Fungi
KW - Heterologous expression
KW - Natural products
UR - http://www.scopus.com/inward/record.url?scp=40949148185&partnerID=8YFLogxK
U2 - 10.1002/cbic.200700390
DO - 10.1002/cbic.200700390
M3 - Article
C2 - 18266306
AN - SCOPUS:40949148185
VL - 9
SP - 585
EP - 594
JO - CHEMBIOCHEM
JF - CHEMBIOCHEM
SN - 1439-4227
IS - 4
ER -