Details
| Original language | English |
|---|---|
| Pages (from-to) | 309-320 |
| Number of pages | 12 |
| Journal | Colloids and Surfaces B: Biointerfaces |
| Volume | 163 |
| Publication status | Published - 29 Dec 2017 |
Abstract
Electrospun poly(ε-caprolactone) (PCL) fiber mats are modified using a chitosan grafted with PCL (CS-g-PCL), to improve the biological performance and to enable further modifications. The graft copolymer is immobilized by the crystallization of the PCL grafts on the PCL fiber surface as binding mechanism. In this way, the surface of the fibers is covered with chitosan bearing cationic amino groups, which allow adsorption of oppositely charged nanoparticulate drug-delivery systems. The modification of the fiber mats and the attachment of the drug delivery systems are easy and scalable dip processes. The process is also versatile; it is possible to attach different polymeric and inorganic nanoparticulate drug-release systems of cationic or anionic nature. The modifications are verified using scanning electron microscopy (SEM) and confocal laser scanning microscopy (CLSM). As proof of principle, the release of ciprofloxacin from silica nanoparticles attached to the modified fiber mats is shown; however, the method is also suited for other biologically active substances including growth factors. The initial cellular attachment and proliferation as well as vitality of the cells is improved by the modification with CS-g-PCL and is further influenced by the type of the drug delivery system attached. Hence, this method can be used to transfer PCL fiber mats into bioactive implants for in-situ tissue engineering applications.
Keywords
- Cell attachment, Electrospinning, Nanoparticulate drug-delivery systems, Poly(ε-caprolactone), Surface modification, Tissue engineering
ASJC Scopus subject areas
- Biochemistry, Genetics and Molecular Biology(all)
- Biotechnology
- Physics and Astronomy(all)
- Surfaces and Interfaces
- Chemistry(all)
- Physical and Theoretical Chemistry
- Chemical Engineering(all)
- Colloid and Surface Chemistry
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In: Colloids and Surfaces B: Biointerfaces, Vol. 163, 29.12.2017, p. 309-320.
Research output: Contribution to journal › Article › Research › peer review
}
TY - JOUR
T1 - Attachment of nanoparticulate drug-release systems on poly(ε-caprolactone) nanofibers via a graftpolymer as interlayer
AU - de Cassan, Dominik
AU - Sydow, Steffen
AU - Schmidt, Nadeschda
AU - Behrens, Peter
AU - Roger, Yvonne
AU - Hoffmann, Andrea
AU - Hoheisel, Anna Lena
AU - Glasmacher, Birgit
AU - Hänsch, Robert
AU - Menzel, Henning
N1 - Funding information: This research project has been supported by the DFG FOR 2180 “Gradierte Implantate für Sehnen-Knochen-Verbindungen“ .
PY - 2017/12/29
Y1 - 2017/12/29
N2 - Electrospun poly(ε-caprolactone) (PCL) fiber mats are modified using a chitosan grafted with PCL (CS-g-PCL), to improve the biological performance and to enable further modifications. The graft copolymer is immobilized by the crystallization of the PCL grafts on the PCL fiber surface as binding mechanism. In this way, the surface of the fibers is covered with chitosan bearing cationic amino groups, which allow adsorption of oppositely charged nanoparticulate drug-delivery systems. The modification of the fiber mats and the attachment of the drug delivery systems are easy and scalable dip processes. The process is also versatile; it is possible to attach different polymeric and inorganic nanoparticulate drug-release systems of cationic or anionic nature. The modifications are verified using scanning electron microscopy (SEM) and confocal laser scanning microscopy (CLSM). As proof of principle, the release of ciprofloxacin from silica nanoparticles attached to the modified fiber mats is shown; however, the method is also suited for other biologically active substances including growth factors. The initial cellular attachment and proliferation as well as vitality of the cells is improved by the modification with CS-g-PCL and is further influenced by the type of the drug delivery system attached. Hence, this method can be used to transfer PCL fiber mats into bioactive implants for in-situ tissue engineering applications.
AB - Electrospun poly(ε-caprolactone) (PCL) fiber mats are modified using a chitosan grafted with PCL (CS-g-PCL), to improve the biological performance and to enable further modifications. The graft copolymer is immobilized by the crystallization of the PCL grafts on the PCL fiber surface as binding mechanism. In this way, the surface of the fibers is covered with chitosan bearing cationic amino groups, which allow adsorption of oppositely charged nanoparticulate drug-delivery systems. The modification of the fiber mats and the attachment of the drug delivery systems are easy and scalable dip processes. The process is also versatile; it is possible to attach different polymeric and inorganic nanoparticulate drug-release systems of cationic or anionic nature. The modifications are verified using scanning electron microscopy (SEM) and confocal laser scanning microscopy (CLSM). As proof of principle, the release of ciprofloxacin from silica nanoparticles attached to the modified fiber mats is shown; however, the method is also suited for other biologically active substances including growth factors. The initial cellular attachment and proliferation as well as vitality of the cells is improved by the modification with CS-g-PCL and is further influenced by the type of the drug delivery system attached. Hence, this method can be used to transfer PCL fiber mats into bioactive implants for in-situ tissue engineering applications.
KW - Cell attachment
KW - Electrospinning
KW - Nanoparticulate drug-delivery systems
KW - Poly(ε-caprolactone)
KW - Surface modification
KW - Tissue engineering
UR - http://www.scopus.com/inward/record.url?scp=85044346679&partnerID=8YFLogxK
U2 - 10.1016/j.colsurfb.2017.12.050
DO - 10.1016/j.colsurfb.2017.12.050
M3 - Article
C2 - 29329076
AN - SCOPUS:85044346679
VL - 163
SP - 309
EP - 320
JO - Colloids and Surfaces B: Biointerfaces
JF - Colloids and Surfaces B: Biointerfaces
SN - 0927-7765
ER -