Details
| Original language | English |
|---|---|
| Pages (from-to) | 1598-1605 |
| Number of pages | 8 |
| Journal | Journal of plant physiology |
| Volume | 167 |
| Issue number | 18 |
| Publication status | Published - 17 Aug 2010 |
Abstract
In plants, the iron storage protein ferritin can be targeted to both chloroplasts and mitochondria. To investigate the role of Arabidopsis ATFER4 ferritin in mitochondrial iron trafficking, atfer4-1 and atfer4-2 mutant knock-outs for the AtFer4 gene were grown in heterotrophic suspension cultures. Both mutants showed altered cell size and morphology, reduced viability, higher H2O2 content and reduced O2 consumption rates when compared to wt. Although no reduction in total ferritin or in mitochondrial ferritin was observed in atfer4 mutants, total iron content increased in atfer4 cells and in atfer4 mitochondria. Transcript correlation analysis highlighted a partial inverse relationship between the transcript levels of the mitochondrial ferric reductase oxidase FRO3, putatively involved in mitochondrial iron import/export, and AtFer4. Consistent with this, FRO3 transcript levels were higher in atfer4 cells. We propose that the complex molecular network maintaining Fe cellular homeostasis requires, in Arabidopsis heterotrophic cells, a proper balance of the different ferritin isoforms, and that alteration of this equilibrium, such as that occurring in atfer4 mutants, is responsible for an altered Fe homeostasis resulting in a change of intraorganellar Fe trafficking.
Keywords
- Arabidopsis, Cell death, Ferritin, Iron, Mitochondria
ASJC Scopus subject areas
- Biochemistry, Genetics and Molecular Biology(all)
- Physiology
- Agricultural and Biological Sciences(all)
- Agronomy and Crop Science
- Agricultural and Biological Sciences(all)
- Plant Science
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In: Journal of plant physiology, Vol. 167, No. 18, 17.08.2010, p. 1598-1605.
Research output: Contribution to journal › Article › Research › peer review
}
TY - JOUR
T1 - AtFer4 ferritin is a determinant of iron homeostasis in Arabidopsis thaliana heterotrophic cells
AU - Tarantino, Delia
AU - Santo, Nadia
AU - Morandini, Piero
AU - Casagrande, Francesca
AU - Braun, Hans Peter
AU - Heinemeyer, Jesco
AU - Vigani, Gianpiero
AU - Soave, Carlo
AU - Murgia, Irene
N1 - Funding information: We thank Valentina Vazzola for help during the cultivation of mutant lines, Ralf Berndt Klösgen and coworkers for communicating unpublished data, Marcello Iriti for the DAB staining and Nicoletta Beffagna for allowing access to all her laboratory equipment, help during mitochondria purification and for her manuscript revision. Janneke Balk kindly donated NAD9 and AOX antibodies to Gianpiero Vigani. We are grateful to Jean Francois Briat and to Frederic Gaymard for the anti-ATFER1 polyclonal antibody and for their valuable comments on the manuscript. This work was supported by MIUR [PRIN 2006, prot. Nr. 2006058818 and PRIN 2008, prot. Nr. 20084XTFBC]. D. Tarantino was partially supported by INGENIO grants.
PY - 2010/8/17
Y1 - 2010/8/17
N2 - In plants, the iron storage protein ferritin can be targeted to both chloroplasts and mitochondria. To investigate the role of Arabidopsis ATFER4 ferritin in mitochondrial iron trafficking, atfer4-1 and atfer4-2 mutant knock-outs for the AtFer4 gene were grown in heterotrophic suspension cultures. Both mutants showed altered cell size and morphology, reduced viability, higher H2O2 content and reduced O2 consumption rates when compared to wt. Although no reduction in total ferritin or in mitochondrial ferritin was observed in atfer4 mutants, total iron content increased in atfer4 cells and in atfer4 mitochondria. Transcript correlation analysis highlighted a partial inverse relationship between the transcript levels of the mitochondrial ferric reductase oxidase FRO3, putatively involved in mitochondrial iron import/export, and AtFer4. Consistent with this, FRO3 transcript levels were higher in atfer4 cells. We propose that the complex molecular network maintaining Fe cellular homeostasis requires, in Arabidopsis heterotrophic cells, a proper balance of the different ferritin isoforms, and that alteration of this equilibrium, such as that occurring in atfer4 mutants, is responsible for an altered Fe homeostasis resulting in a change of intraorganellar Fe trafficking.
AB - In plants, the iron storage protein ferritin can be targeted to both chloroplasts and mitochondria. To investigate the role of Arabidopsis ATFER4 ferritin in mitochondrial iron trafficking, atfer4-1 and atfer4-2 mutant knock-outs for the AtFer4 gene were grown in heterotrophic suspension cultures. Both mutants showed altered cell size and morphology, reduced viability, higher H2O2 content and reduced O2 consumption rates when compared to wt. Although no reduction in total ferritin or in mitochondrial ferritin was observed in atfer4 mutants, total iron content increased in atfer4 cells and in atfer4 mitochondria. Transcript correlation analysis highlighted a partial inverse relationship between the transcript levels of the mitochondrial ferric reductase oxidase FRO3, putatively involved in mitochondrial iron import/export, and AtFer4. Consistent with this, FRO3 transcript levels were higher in atfer4 cells. We propose that the complex molecular network maintaining Fe cellular homeostasis requires, in Arabidopsis heterotrophic cells, a proper balance of the different ferritin isoforms, and that alteration of this equilibrium, such as that occurring in atfer4 mutants, is responsible for an altered Fe homeostasis resulting in a change of intraorganellar Fe trafficking.
KW - Arabidopsis
KW - Cell death
KW - Ferritin
KW - Iron
KW - Mitochondria
UR - http://www.scopus.com/inward/record.url?scp=78149412309&partnerID=8YFLogxK
U2 - 10.1016/j.jplph.2010.06.020
DO - 10.1016/j.jplph.2010.06.020
M3 - Article
C2 - 20724023
AN - SCOPUS:78149412309
VL - 167
SP - 1598
EP - 1605
JO - Journal of plant physiology
JF - Journal of plant physiology
SN - 0176-1617
IS - 18
ER -