Details
| Original language | English |
|---|---|
| Article number | ovae076 |
| Journal | Letters in applied microbiology |
| Volume | 77 |
| Issue number | 8 |
| Early online date | 6 Aug 2024 |
| Publication status | Published - Aug 2024 |
Abstract
The reaction kinetics of lithotrophic ammonia-oxidizing bacteria (AOB) are strongly dependent on dissolved oxygen (DO) as their metabolism is an aerobic process. In this study, we estimate the kinetic parameters, including the oxygen affinity constant (Km[O2]) and the maximum oxygen consumption rate (Vmax[O2]), of different AOB species, by fitting the data to the Michaelis–Menten equation using nonlinear regression analysis. An example for three different species of Nitrosomonas bacteria (N. europaea, N. eutropha, and N. mobilis) in monoculture is given, finding a Km[O2] of 0.25 ± 0.05 mg l−1, 0.47 ± 0.09 mg l−1, and 0.28 ± 0.08 mg l−1, and a Vmax[O2] of 0.07 ± 0.04 pg h−1cell−1, 0.25 ± 0.06 pg h−1cell−1, and 0.02 ± 0.001 pg h−1cell−1 for N. europaea, N. eutropha, and N. mobilis, respectively. This study shows that of the analyzed AOB, N. europaea has the highest affinity towards oxygen and N. eutropha the lowest affinity towards oxygen, indicating that the former can convert ammonia even under low DO conditions. These results improve the understanding of the ecophysiology of AOB in the environment. The accuracy of mathematically modelled ammonia oxidation can be improved, allowing the implementation of better management practices to restore the nitrogen cycle in natural and engineered water systems.
Keywords
- ammonia oxidizing bacteria (aob), kinetics, non-linear regression analysis, oxygen affinity constant, parameter estimation
ASJC Scopus subject areas
- Immunology and Microbiology(all)
- Applied Microbiology and Biotechnology
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In: Letters in applied microbiology, Vol. 77, No. 8, ovae076, 08.2024.
Research output: Contribution to journal › Article › Research › peer review
}
TY - JOUR
T1 - Assessment of oxygen kinetic parameters for closely related ammonia-oxidizing bacteria
AU - Saha, Pallabita
AU - Kniggendorf, Ann Kathrin
AU - Pommerening-Röser, Andreas
AU - Nogueira, Regina
N1 - Publisher Copyright: © The Author(s) 2024. Published by Oxford University Press on behalf of Applied Microbiology International. All rights reserved.
PY - 2024/8
Y1 - 2024/8
N2 - The reaction kinetics of lithotrophic ammonia-oxidizing bacteria (AOB) are strongly dependent on dissolved oxygen (DO) as their metabolism is an aerobic process. In this study, we estimate the kinetic parameters, including the oxygen affinity constant (Km[O2]) and the maximum oxygen consumption rate (Vmax[O2]), of different AOB species, by fitting the data to the Michaelis–Menten equation using nonlinear regression analysis. An example for three different species of Nitrosomonas bacteria (N. europaea, N. eutropha, and N. mobilis) in monoculture is given, finding a Km[O2] of 0.25 ± 0.05 mg l−1, 0.47 ± 0.09 mg l−1, and 0.28 ± 0.08 mg l−1, and a Vmax[O2] of 0.07 ± 0.04 pg h−1cell−1, 0.25 ± 0.06 pg h−1cell−1, and 0.02 ± 0.001 pg h−1cell−1 for N. europaea, N. eutropha, and N. mobilis, respectively. This study shows that of the analyzed AOB, N. europaea has the highest affinity towards oxygen and N. eutropha the lowest affinity towards oxygen, indicating that the former can convert ammonia even under low DO conditions. These results improve the understanding of the ecophysiology of AOB in the environment. The accuracy of mathematically modelled ammonia oxidation can be improved, allowing the implementation of better management practices to restore the nitrogen cycle in natural and engineered water systems.
AB - The reaction kinetics of lithotrophic ammonia-oxidizing bacteria (AOB) are strongly dependent on dissolved oxygen (DO) as their metabolism is an aerobic process. In this study, we estimate the kinetic parameters, including the oxygen affinity constant (Km[O2]) and the maximum oxygen consumption rate (Vmax[O2]), of different AOB species, by fitting the data to the Michaelis–Menten equation using nonlinear regression analysis. An example for three different species of Nitrosomonas bacteria (N. europaea, N. eutropha, and N. mobilis) in monoculture is given, finding a Km[O2] of 0.25 ± 0.05 mg l−1, 0.47 ± 0.09 mg l−1, and 0.28 ± 0.08 mg l−1, and a Vmax[O2] of 0.07 ± 0.04 pg h−1cell−1, 0.25 ± 0.06 pg h−1cell−1, and 0.02 ± 0.001 pg h−1cell−1 for N. europaea, N. eutropha, and N. mobilis, respectively. This study shows that of the analyzed AOB, N. europaea has the highest affinity towards oxygen and N. eutropha the lowest affinity towards oxygen, indicating that the former can convert ammonia even under low DO conditions. These results improve the understanding of the ecophysiology of AOB in the environment. The accuracy of mathematically modelled ammonia oxidation can be improved, allowing the implementation of better management practices to restore the nitrogen cycle in natural and engineered water systems.
KW - ammonia oxidizing bacteria (aob)
KW - kinetics
KW - non-linear regression analysis
KW - oxygen affinity constant
KW - parameter estimation
UR - http://www.scopus.com/inward/record.url?scp=85202082445&partnerID=8YFLogxK
U2 - 10.1093/lambio/ovae076
DO - 10.1093/lambio/ovae076
M3 - Article
C2 - 39108081
AN - SCOPUS:85202082445
VL - 77
JO - Letters in applied microbiology
JF - Letters in applied microbiology
SN - 0266-8254
IS - 8
M1 - ovae076
ER -