Details
| Original language | English |
|---|---|
| Journal | Journal of biophotonics |
| Early online date | 16 Feb 2025 |
| Publication status | E-pub ahead of print - 16 Feb 2025 |
Abstract
Keratoconus and other corneal ectatic disorders involve the degradation of collagen fibers, which compromises the corneal biomechanical properties. Ultraviolet-A (UVA) crosslinking has emerged as the primary treatment to slow down collagen degradation. This treatment is limited in both penetration depth and spatial precision, potentially leading to unwanted side effects. This study compares the changes in biomechanical properties of corneas crosslinked with UVA irradiation and a near-infrared femtosecond laser, using Brillouin microscopy. The biomechanical properties of the crosslinked regions were mapped in terms of Brillouin frequency shift in three dimensions. UVA crosslinking showed an average increase in Brillouin frequency shift of ~100 MHz. We demonstrate targeted spatial and axial corneal femtosecond crosslinking, with similar Brillouin frequency shift values to UVA in crosslinked regions.
Keywords
- Brillouin microscopy, collagen type 1, cornea, femtosecond crosslinking, keratectasia, UVA crosslinking
ASJC Scopus subject areas
- Chemistry(all)
- General Chemistry
- Materials Science(all)
- General Materials Science
- Biochemistry, Genetics and Molecular Biology(all)
- General Biochemistry,Genetics and Molecular Biology
- Engineering(all)
- General Engineering
- Physics and Astronomy(all)
- General Physics and Astronomy
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In: Journal of biophotonics, 16.02.2025.
Research output: Contribution to journal › Article › Research › peer review
}
TY - JOUR
T1 - Assessing UVA and Laser-Induced Crosslinking via Brillouin Microscopy
AU - Iriarte-Valdez, Christian A.
AU - Wenzel, Johannes
AU - Baron, Emilie
AU - Claus, Alexandra Y.
AU - Kalies, Stefan
AU - Sperlich, Karsten
AU - Stachs, Oliver
AU - Torres-Mapa, Maria Leilani
AU - Heisterkamp, Alexander
N1 - Publisher Copyright: © 2025 The Author(s). Journal of Biophotonics published by Wiley-VCH GmbH.
PY - 2025/2/16
Y1 - 2025/2/16
N2 - Keratoconus and other corneal ectatic disorders involve the degradation of collagen fibers, which compromises the corneal biomechanical properties. Ultraviolet-A (UVA) crosslinking has emerged as the primary treatment to slow down collagen degradation. This treatment is limited in both penetration depth and spatial precision, potentially leading to unwanted side effects. This study compares the changes in biomechanical properties of corneas crosslinked with UVA irradiation and a near-infrared femtosecond laser, using Brillouin microscopy. The biomechanical properties of the crosslinked regions were mapped in terms of Brillouin frequency shift in three dimensions. UVA crosslinking showed an average increase in Brillouin frequency shift of ~100 MHz. We demonstrate targeted spatial and axial corneal femtosecond crosslinking, with similar Brillouin frequency shift values to UVA in crosslinked regions.
AB - Keratoconus and other corneal ectatic disorders involve the degradation of collagen fibers, which compromises the corneal biomechanical properties. Ultraviolet-A (UVA) crosslinking has emerged as the primary treatment to slow down collagen degradation. This treatment is limited in both penetration depth and spatial precision, potentially leading to unwanted side effects. This study compares the changes in biomechanical properties of corneas crosslinked with UVA irradiation and a near-infrared femtosecond laser, using Brillouin microscopy. The biomechanical properties of the crosslinked regions were mapped in terms of Brillouin frequency shift in three dimensions. UVA crosslinking showed an average increase in Brillouin frequency shift of ~100 MHz. We demonstrate targeted spatial and axial corneal femtosecond crosslinking, with similar Brillouin frequency shift values to UVA in crosslinked regions.
KW - Brillouin microscopy
KW - collagen type 1
KW - cornea
KW - femtosecond crosslinking
KW - keratectasia
KW - UVA crosslinking
UR - http://www.scopus.com/inward/record.url?scp=85219665258&partnerID=8YFLogxK
U2 - 10.1002/jbio.202400401
DO - 10.1002/jbio.202400401
M3 - Article
AN - SCOPUS:85219665258
JO - Journal of biophotonics
JF - Journal of biophotonics
SN - 1864-063X
ER -