Details
| Original language | English |
|---|---|
| Pages (from-to) | 1-10 |
| Number of pages | 10 |
| Journal | PLANTA |
| Volume | 217 |
| Issue number | 1 |
| Publication status | Published - 1 May 2003 |
Abstract
Sulfurtransferases (STs) and β-cyano-L-alanine synthase (CAS) are suggested to be involved in cyanide detoxification. Therefore, the accumulation of ST1 and CAS RNAs, and the ST and CAS protein levels and enzyme activities were determined in Arabidopsis thaliana Heynh. plants grown under different conditions. Senescence-associated processes were successfully induced by natural aging, by jasmonate methyl ester and by darkness in whole plants and detached leaves, as demonstrated by the expression of the senescence marker genes SAG12 and SAG13. However, the changes in RNA accumulation and protein levels of ST and CAS did not correlate with the expression of these senescence marker genes; the specific ST and CAS activities either decreased (ST) or increased (CAS). In another experiment, Arabidopsis plants were sprayed with cyanide to investigate the role of ST and CAS in cyanide detoxification. The expression of ST and CAS at the RNA and protein levels, and also the enzyme activities, remained equal in cyanide-treated and control plants. Incubation with 1-aminocyclopropane-1-carboxylic acid, the precursor of ethylene, increased while fumigation with ethylene decreased expression and activity of ST and CAS. In summary, cyanide does not induce the expression or enhance the activity of ST and CAS in Arabidopsis. For both proteins the evidence for a role in cyanide detoxification or induced senescence is low.
Keywords
- 3-Mercaptopyruvate, Arabidopsis, Cyanide detoxification, Ethylene, Jasmonate, Senescence
ASJC Scopus subject areas
- Biochemistry, Genetics and Molecular Biology(all)
- Genetics
- Agricultural and Biological Sciences(all)
- Plant Science
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In: PLANTA, Vol. 217, No. 1, 01.05.2003, p. 1-10.
Research output: Contribution to journal › Article › Research › peer review
}
TY - JOUR
T1 - Arabidopsis sulfurtransferases
T2 - Investigation of their function during senescence and in cyanide detoxification
AU - Meyer, Tanja
AU - Burow, Meike
AU - Bauer, Michael
AU - Papenbrock, Jutta
N1 - Funding information: We thank Pamela von Trzebiatowski and Julia Volker for their excellent technical assistance, Dr. T. Winkelmann (Institute of Floriculture, Hannover, Germany) for assistance with the ethylene fumigation experiment, and the gardeners for growing the plants. We acknowledge the work of the undergraduate student Till Berger (Fribourg, Switzerland) who analyzed the cyanide contents during a stay in our laboratory. We are grateful to Prof. R. Amasino (Department of Biochemistry, Madison, USA) for providing the SAG12 and SAG13 cDNA clones, and Prof. K. Saito (Institute for Pharmaceutical Sciences, Chiba, Japan) for sending the CAS cDNA clone. We appreciate the suggestions of two anonymous reviewers who helped to improve the manuscript. The work was financially supported by the Deutsche Forschungs-gemeinschaft (PA 764/1-1/2, SCHM 307/15-1) and the Fonds der Chemischen Industrie.
PY - 2003/5/1
Y1 - 2003/5/1
N2 - Sulfurtransferases (STs) and β-cyano-L-alanine synthase (CAS) are suggested to be involved in cyanide detoxification. Therefore, the accumulation of ST1 and CAS RNAs, and the ST and CAS protein levels and enzyme activities were determined in Arabidopsis thaliana Heynh. plants grown under different conditions. Senescence-associated processes were successfully induced by natural aging, by jasmonate methyl ester and by darkness in whole plants and detached leaves, as demonstrated by the expression of the senescence marker genes SAG12 and SAG13. However, the changes in RNA accumulation and protein levels of ST and CAS did not correlate with the expression of these senescence marker genes; the specific ST and CAS activities either decreased (ST) or increased (CAS). In another experiment, Arabidopsis plants were sprayed with cyanide to investigate the role of ST and CAS in cyanide detoxification. The expression of ST and CAS at the RNA and protein levels, and also the enzyme activities, remained equal in cyanide-treated and control plants. Incubation with 1-aminocyclopropane-1-carboxylic acid, the precursor of ethylene, increased while fumigation with ethylene decreased expression and activity of ST and CAS. In summary, cyanide does not induce the expression or enhance the activity of ST and CAS in Arabidopsis. For both proteins the evidence for a role in cyanide detoxification or induced senescence is low.
AB - Sulfurtransferases (STs) and β-cyano-L-alanine synthase (CAS) are suggested to be involved in cyanide detoxification. Therefore, the accumulation of ST1 and CAS RNAs, and the ST and CAS protein levels and enzyme activities were determined in Arabidopsis thaliana Heynh. plants grown under different conditions. Senescence-associated processes were successfully induced by natural aging, by jasmonate methyl ester and by darkness in whole plants and detached leaves, as demonstrated by the expression of the senescence marker genes SAG12 and SAG13. However, the changes in RNA accumulation and protein levels of ST and CAS did not correlate with the expression of these senescence marker genes; the specific ST and CAS activities either decreased (ST) or increased (CAS). In another experiment, Arabidopsis plants were sprayed with cyanide to investigate the role of ST and CAS in cyanide detoxification. The expression of ST and CAS at the RNA and protein levels, and also the enzyme activities, remained equal in cyanide-treated and control plants. Incubation with 1-aminocyclopropane-1-carboxylic acid, the precursor of ethylene, increased while fumigation with ethylene decreased expression and activity of ST and CAS. In summary, cyanide does not induce the expression or enhance the activity of ST and CAS in Arabidopsis. For both proteins the evidence for a role in cyanide detoxification or induced senescence is low.
KW - 3-Mercaptopyruvate
KW - Arabidopsis
KW - Cyanide detoxification
KW - Ethylene
KW - Jasmonate
KW - Senescence
UR - http://www.scopus.com/inward/record.url?scp=0038610902&partnerID=8YFLogxK
M3 - Article
C2 - 12721843
AN - SCOPUS:0038610902
VL - 217
SP - 1
EP - 10
JO - PLANTA
JF - PLANTA
SN - 0032-0935
IS - 1
ER -