Details
| Original language | English |
|---|---|
| Article number | 101 |
| Journal | Frontiers in Plant Science |
| Volume | 4 |
| Issue number | APR |
| Publication status | Published - 24 Apr 2013 |
Abstract
The analytical depth of investigation of the peroxisomal proteome of the model plant Arabidopsis thaliana has not yet reached that of other major cellular organelles such as chloroplasts or mitochondria. This is primarily due to the difficulties associated with isolating and obtaining purified samples of peroxisomes from Arabidopsis. So far only a handful of research groups have been successful in obtaining such fractions. To make things worse, enriched peroxisome fractions frequently suffer from significant organellar contamination, lowering confidence in localization assignment of the identified proteins. As with other cellular compartments, identification of peroxisomal proteins forms the basis for investigations of the dynamics of the peroxisomal proteome. It is therefore not surprising that, in terms of functional analyses by proteomic means, peroxisomes are lagging considerably behind chloroplasts or mitochondria. Alternative strategies are needed to overcome the obstacle of hard-to-obtain organellar fractions. This will help to close the knowledge gap between peroxisomes and other organelles and provide a full picture of the physiological pathways shared between organelles. In this review, we briefly summarize the status quo and discuss some of the methodological alternatives to classic organelle proteomic approaches.
Keywords
- Free-flow electrophoresis, Functional proteomics, Peroxisome, Protein:protein interaction, Subcellular localization, Targeted quantitation of proteins
ASJC Scopus subject areas
- Agricultural and Biological Sciences(all)
- Plant Science
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In: Frontiers in Plant Science, Vol. 4, No. APR, 101, 24.04.2013.
Research output: Contribution to journal › Review article › Research › peer review
}
TY - JOUR
T1 - Arabidopsis peroxisome proteomics
AU - Bussell, John D.
AU - Behrens, Christof
AU - Ecke, Wiebke
AU - Eubel, Holger
N1 - Copyright: Copyright 2014 Elsevier B.V., All rights reserved.
PY - 2013/4/24
Y1 - 2013/4/24
N2 - The analytical depth of investigation of the peroxisomal proteome of the model plant Arabidopsis thaliana has not yet reached that of other major cellular organelles such as chloroplasts or mitochondria. This is primarily due to the difficulties associated with isolating and obtaining purified samples of peroxisomes from Arabidopsis. So far only a handful of research groups have been successful in obtaining such fractions. To make things worse, enriched peroxisome fractions frequently suffer from significant organellar contamination, lowering confidence in localization assignment of the identified proteins. As with other cellular compartments, identification of peroxisomal proteins forms the basis for investigations of the dynamics of the peroxisomal proteome. It is therefore not surprising that, in terms of functional analyses by proteomic means, peroxisomes are lagging considerably behind chloroplasts or mitochondria. Alternative strategies are needed to overcome the obstacle of hard-to-obtain organellar fractions. This will help to close the knowledge gap between peroxisomes and other organelles and provide a full picture of the physiological pathways shared between organelles. In this review, we briefly summarize the status quo and discuss some of the methodological alternatives to classic organelle proteomic approaches.
AB - The analytical depth of investigation of the peroxisomal proteome of the model plant Arabidopsis thaliana has not yet reached that of other major cellular organelles such as chloroplasts or mitochondria. This is primarily due to the difficulties associated with isolating and obtaining purified samples of peroxisomes from Arabidopsis. So far only a handful of research groups have been successful in obtaining such fractions. To make things worse, enriched peroxisome fractions frequently suffer from significant organellar contamination, lowering confidence in localization assignment of the identified proteins. As with other cellular compartments, identification of peroxisomal proteins forms the basis for investigations of the dynamics of the peroxisomal proteome. It is therefore not surprising that, in terms of functional analyses by proteomic means, peroxisomes are lagging considerably behind chloroplasts or mitochondria. Alternative strategies are needed to overcome the obstacle of hard-to-obtain organellar fractions. This will help to close the knowledge gap between peroxisomes and other organelles and provide a full picture of the physiological pathways shared between organelles. In this review, we briefly summarize the status quo and discuss some of the methodological alternatives to classic organelle proteomic approaches.
KW - Free-flow electrophoresis
KW - Functional proteomics
KW - Peroxisome
KW - Protein:protein interaction
KW - Subcellular localization
KW - Targeted quantitation of proteins
UR - http://www.scopus.com/inward/record.url?scp=84896830318&partnerID=8YFLogxK
U2 - 10.3389/fpls.2013.00101
DO - 10.3389/fpls.2013.00101
M3 - Review article
AN - SCOPUS:84896830318
VL - 4
JO - Frontiers in Plant Science
JF - Frontiers in Plant Science
SN - 1664-462X
IS - APR
M1 - 101
ER -