Details
| Original language | English |
|---|---|
| Pages (from-to) | 107-119 |
| Number of pages | 13 |
| Journal | Advances in Biochemical Engineering/Biotechnology |
| Volume | 170 |
| Publication status | Published - 8 Mar 2019 |
Abstract
Aptazymes are synthetic molecules composed of an aptamer domain and a catalytic active nucleic acid unit, which may be a ribozyme or a DNAzyme. In these constructs the aptamer domain serves as a molecular switch that can regulate the catalytic activity of the ribozyme or DNAzyme subunit. This regulation is triggered by binding of the aptamers target molecule, which causes significant structural changes in the aptamer and thus in the entire aptazyme. Therefore, aptazymes function similar to allosteric enzymes, whose catalytic activity is regulated by binding of ligands (effectors) to allosteric sites due to alteration of the three-dimensional structure of the active site of the enzyme. In case of aptazymes, the allosteric site is composed of an aptamer. Aptazymes can be designed for different applications and have already been used in analytical assays as well as for the regulation of gene expression.
Keywords
- Aptamers, Nucleotide/chemistry, Catalysis, DNA, Catalytic/chemistry, Ligands, Protein Binding, RNA, Catalytic/chemistry, DNAzyme, Ribozyme, Aptamer, Aptazyme
ASJC Scopus subject areas
- Immunology and Microbiology(all)
- Applied Microbiology and Biotechnology
- Chemical Engineering(all)
- Bioengineering
- Biochemistry, Genetics and Molecular Biology(all)
- Biotechnology
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In: Advances in Biochemical Engineering/Biotechnology, Vol. 170, 08.03.2019, p. 107-119.
Research output: Contribution to journal › Review article › Research › peer review
}
TY - JOUR
T1 - Aptazymes
T2 - Expanding the Specificity of Natural Catalytic Nucleic Acids by Application of In Vitro Selected Oligonucleotides
AU - Walter, Johanna-Gabriela
AU - Stahl, Frank
PY - 2019/3/8
Y1 - 2019/3/8
N2 - Aptazymes are synthetic molecules composed of an aptamer domain and a catalytic active nucleic acid unit, which may be a ribozyme or a DNAzyme. In these constructs the aptamer domain serves as a molecular switch that can regulate the catalytic activity of the ribozyme or DNAzyme subunit. This regulation is triggered by binding of the aptamers target molecule, which causes significant structural changes in the aptamer and thus in the entire aptazyme. Therefore, aptazymes function similar to allosteric enzymes, whose catalytic activity is regulated by binding of ligands (effectors) to allosteric sites due to alteration of the three-dimensional structure of the active site of the enzyme. In case of aptazymes, the allosteric site is composed of an aptamer. Aptazymes can be designed for different applications and have already been used in analytical assays as well as for the regulation of gene expression.
AB - Aptazymes are synthetic molecules composed of an aptamer domain and a catalytic active nucleic acid unit, which may be a ribozyme or a DNAzyme. In these constructs the aptamer domain serves as a molecular switch that can regulate the catalytic activity of the ribozyme or DNAzyme subunit. This regulation is triggered by binding of the aptamers target molecule, which causes significant structural changes in the aptamer and thus in the entire aptazyme. Therefore, aptazymes function similar to allosteric enzymes, whose catalytic activity is regulated by binding of ligands (effectors) to allosteric sites due to alteration of the three-dimensional structure of the active site of the enzyme. In case of aptazymes, the allosteric site is composed of an aptamer. Aptazymes can be designed for different applications and have already been used in analytical assays as well as for the regulation of gene expression.
KW - Aptamers, Nucleotide/chemistry
KW - Catalysis
KW - DNA, Catalytic/chemistry
KW - Ligands
KW - Protein Binding
KW - RNA, Catalytic/chemistry
KW - DNAzyme
KW - Ribozyme
KW - Aptamer
KW - Aptazyme
UR - http://www.scopus.com/inward/record.url?scp=85072745912&partnerID=8YFLogxK
U2 - 10.1007/10_2019_92
DO - 10.1007/10_2019_92
M3 - Review article
C2 - 30847536
VL - 170
SP - 107
EP - 119
JO - Advances in Biochemical Engineering/Biotechnology
JF - Advances in Biochemical Engineering/Biotechnology
SN - 0724-6145
ER -